Vratislav Horák

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

JČ

Jana Čížková

Czech Academy of Sciences, Institute of Experimental Botany

Vojtěch Adam

Mendel University in Brno

Claudine Geffrotin

AgroParisTech

Jan Hrádecký

University of Ostrava

Helena Kupcová Skalníková

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

K Fortýn

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

Ján Strnádel

Martin University Hospital

Silvia Vincent‐Naulleau

Génétique Animale et Biologie Intégrative

A Palanová

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

René Kizek

Research Institute of Inorganic Chemistry

Cooperative Institutions

Charles University

Czech Academy of Sciences

Czech Academy of Sciences, Institute of Animal Physiology and Genetics

Mendel University in Brno

Brno University of Technology

Masaryk University

Central European Institute of Technology

Czech Academy of Sciences, Institute of Microbiology

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Institut National de la Recherche Agronomique

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Dysbiosis of skin microbiome and gut microbiome in melanoma progression

BMC Microbiology (2022)

Chahrazed Mekadim Helena Kupcová Skalníková Jana Čížková Veronika Cizkova A Palanová

Abstract Background The microbiome alterations are associated with cancer growth and may influence the immune system and response to therapy. Particularly, the gut microbiome has been recently shown to modulate response to melanoma immunotherapy. However, the role of the skin microbiome has not been well explored in the skin tumour microenvironment and the link between the gut microbiome and skin microbiome has not been investigated in melanoma progression. Therefore, the aim of the present study was to examine associations between dysbiosis in the skin and gut microbiome and the melanoma growth using MeLiM porcine model of melanoma progression and spontaneous regression. Results Parallel analysis of cutaneous microbiota and faecal microbiota of the same individuals was performed in 8 to 12 weeks old MeLiM piglets. The bacterial composition of samples was analysed by high throughput sequencing of the V4-V5 region of the 16S rRNA gene. A significant difference in microbiome diversity and richness between melanoma tissue and healthy skin and between the faecal microbiome of MeLiM piglets and control piglets were observed. Both Principal Coordinate Analysis and Non-metric multidimensional scaling revealed dissimilarities between different bacterial communities. Linear discriminant analysis effect size at the genus level determined different potential biomarkers in multiple bacterial communities. Lactobacillus, Clostridium sensu stricto 1 and Corynebacterium 1 were the most discriminately higher genera in the healthy skin microbiome, while Fusobacterium, Trueperella, Staphylococcus, Streptococcus and Bacteroides were discriminately abundant in melanoma tissue microbiome. Bacteroides, Fusobacterium and Escherichia-Shigella were associated with the faecal microbiota of MeLiM piglets. Potential functional pathways analysis based on the KEGG database indicated significant differences in the predicted profile metabolisms between the healthy skin microbiome and melanoma tissue microbiome. The faecal microbiome of MeLiM piglets was enriched by genes related to membrane transports pathways allowing for the increase of intestinal permeability and alteration of the intestinal mucosal barrier. Conclusion The associations between melanoma progression and dysbiosis in the skin microbiome as well as dysbiosis in the gut microbiome were identified. Results provide promising information for further studies on the local skin and gut microbiome involvement in melanoma progression and may support the development of new therapeutic approaches.

Dysbiosis

Gut microbiome

Parasitology

10.1186/s12866-022-02458-5

Cite

Citations (56)

A genome-wide scan for familial melanoma in the MeLiM swine model

HAL (Le Centre pour la Communication Scientifique Directe) (2002)

Claudine Geffrotin Françoise Créchet Catherine Le Chalony Pascale Le Roy Jean Jacques J. J. Leplat

Genome Scan

Source

Cite

Citations (0)

Histochemical fiber types in the thigh muscles of 4 chicken inbred lines.

PubMed (1989)

Vratislav Horák K Sevcíková H. Knížetová

Distribution, frequency and diameter of fiber types were ascertained in the thigh muscles of I, C, W and M chicken inbred lines. The iliotibialis posterior muscle is composed of FOG and FG types uniformly distributed over the whole muscle cross-section. Three fiber types are demonstrated in the sartorius and semimembranosus muscles. In the superficial regions, the FOG and FG types are present only. Avian SO type appears down into the depth of the muscles. The frequency of this type and FOG type gradually increases in this direction whereas the proportion of FG type decreases. Semimembranosus muscle shows generally higher percentage of FOG fibers and lower frequency of remaining two types. Differences between inbred lines in fiber type frequencies are mostly insignificant. However, the M line with the highest live and muscle weights shows larger fiber diameter of FG and FOG types than I, C and W lines.

Fiber type

Muscle fibre

Source

Cite

Citations (12)

PP-26 Serum enzyme activities in monitoring melim melanoma progression and regression

Pigment Cell Research (2003)

J Borovanský Vratislav Horák Jana Uhrová Tomáš Zima M. Elleder

10.1034/j.1600-0749.2003.08391.x

Cite

Citations (1)

Phage typing coagulase-positive staphylococci from rooks and gulls

Research in Veterinary Science (1988)

Václav Hájek Vratislav Horák J. BALUSEK

Phage typing

Coagulase

10.1016/s0034-5288(18)30849-x

Cite

Citations (5)

Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma

International Journal of Electrochemical Science (2012)

Monika Kremplová Ludmila Krejčová David Hynek Péter Baráth Petr Majzlík

Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of μl of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 μg Fe ions/ml) and group of animals suffering from melanoma progression (3.5 μg Fe ions/ml) were determined.

Repeatability

Supporting electrolyte

10.1016/s1452-3981(23)19450-8

Cite

Citations (11)

Importance of colicinogeny for the course of acute bacillary dysentery.

Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, Abt. I (1979)

Jan Bureš Vratislav Horák J Duben

Bacillary dysentery

Source

Cite

Citations (0)

Influence of exogenous transferrin on chicken skeletal muscles.

Czech Journal of Animal Science (2000)

Vratislav Horák C. Rehfeldt A. Stratil Petr Bobák

Source

Cite

Citations (0)

In vivo effects of focused shock waves on tumor tissue visualized by fluorescence staining techniques

Bioelectrochemistry (2014)

Petr Lukeš J. Zeman Vratislav Horák Petr Hoffer P Poučková

10.1016/j.bioelechem.2014.08.019

Cite

Citations (12)

[Drug therapy in homes for retired persons].

PubMed (1975)

Květoslav Šipr Vratislav Horák

The authors analyzed the prescriptions in an old age pensioners home in Rokytnice near Prerov (240 people) in 1966-1972 and the assembled results were compared with drug expenditure in an old age pensioners home in Radkova Lhota (102 people) in 1972). Almost half the people in these homes suffer from at least three diseases which must be considered during drug pprscription; the great moribidity explains the almost fivefold expenditure on drugs per head, as compared with ambulatory care. Prescription of psychopharmaceutical preparations, antibiotics, analgetics and bronchodilatating drugs corresponds to the principles of expedient pharmacotherapy. More attention must be devoted to the dosage of cardiotonics. Geriatric drugs and vitamins, specially ascorbic acid are prescribed little.

Pharmacotherapy

Geriatric Care

Elderly people

Source

Cite

Citations (0)