Laurent Thouin

Sorbonne Université

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Christian Amatore

École Normale Supérieure - PSL

C. Sella

Sorbonne Université

Fethi Bédioui

Chimie ParisTech

Cécile Pebay

École Normale Supérieure - PSL

Sophie Griveau

Chimie ParisTech

Sabine Szunerits

Université de Lille

Raquel Oliveira

University of Minho

Thomas Abadie

University of Birmingham

Jean-Stéphane Warkocz

École Normale Supérieure - PSL

Frédéric Lemaître

Centre National de la Recherche Scientifique

Cooperative Institutions

Centre National de la Recherche Scientifique

126

Sorbonne Université

École Normale Supérieure - PSL

Université Paris Sciences et Lettres

Chimie ParisTech

Université Paris Cité

Processus d'Activation Sélective par Transfert d'Énergie Uni-électronique ou Radiatif

École Normale Supérieure

Inserm

Université Paris-Saclay

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Simultaneous Electrochemical Detection of Primary Reactive Oxygen and Nitrogen Species Released by Cell Populations in Integrated Microdevices

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Yun Li C. Sella Frédéric Lemaître Manon Guille‐Collignon Christian Amatore

Innovative microdevices were designed to monitor electrochemically primary reactive oxygen (ROS) and reactive nitrogen species (RNS) released by populations of aerobic cells. Taking advantage of the space confinement and microelectrodes performances, only few experiments were sufficient to provide significant statistical data relative to the average behavior of cells during oxidative stress bursts. Platinum-black coated platinum (Pt/Pt-black) electrodes were microfabricated and optimized to achieve optimal performance during the electrochemical detection of four primary species H2O2, NO, ONOO- and NO2-.1,2 The results demonstrated that relative ROS/RNS contents in synthetic mixtures can be easily assessed at selected detection potentials. Under given experimental conditions, the Pt/Pt-black electrodes allow detection limits down to 10 nM with high sensitivities and long-term stability of the electrodes responses. The electrochemical detection of ROS/RNS released by cell populations was then implemented in a multi-chambers microsystem3 and in a microfluidics device.4 As an important cell type, RAW 264.7 macrophages secretion triggered by a calcium ionophore was chosen for assessing the performance, sensitivity and specificity of the detection in both cases. In comparison to some previous evaluations obtained from single-cell measurements, reproducible and relevant determinations could be achieved. However, separating emitting cells from the detection area in the microfluidic device seems to be a better approach to avoid any perturbations of cell behaviors by electrode operations. Furthermore, any biological effects during oxidative stress of living cells can be easily investigated. As a proof of concept, we reported the analysis of the influence of a NO synthase inhibitor during the perfusion culture.

Reactive nitrogen species

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Effects of chemical environment on diffusivities within thin Nafion® films as monitored from chronoamperometric responses of generator–collector double microband assemblies

Journal of Electroanalytical Chemistry (2003)

Christian Amatore C. Sella Laurent Thouin

Nafion

Chronoamperometry

10.1016/s0022-0728(03)00195-5

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Citations (10)

Comparison of Arrayed Ultramicroelectrodes Array for Direct Detection of NO-Release from S-Nitrosoglutathione

A. Nassi L. To Thi Kim Arlette Girard L. Griscom Florence Razan

S-Nitrosoglutathione

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Sedimentation-based confinement of individual Giant Unilamellar Vesicles in microchamber arrays with a dynamically exchangeable outer medium

Syed Kaabir Ali C. Sella Vadim Dilhas Barbara Jacková Marina Mariconti

Giant unilamellar vesicles (GUVs) are an ideal model to study cellular membrane functions in vitro, yet difficult to manipulate due to their fragile nature, especially when subjected to dynamic change of their external microenvironment. Here, we introduce an original microfluidic concept for constrain-free confinement of individual GUVs in microchambers with a dynamically exchangeable outer medium. With this method, GUVs self-confine in an array of laterally separated microchambers by sedimentation, avoiding any mechanical constrain and membrane deformation while allowing time-resolved microscopy observation. A microfluidic channel above the chambers allows a diffusion-based exchange of the GUV outer medium that can be completed in a few seconds for fast-diffusing molecules to about one minute for large proteins in a viscous medium. We numerically establish the geometric and flow parameters optimizing medium exchange while preventing GUV from lifting out. We experimentally demonstrate that different aqueous solutions separated by air plugs can be flowed into the channel by taking advantage of a polydimethylsiloxane-based hydrophilic channel wall. We also exploit the possibility to manipulate microliter sample volumes and dynamically control the external environment of GUV for in situ observation of membrane binding protein cell-free expression. We find in particular that the membrane-targeting sequence of Bacillus subtilis MinD binds to GUVs and induces extensive membrane tubulation. This technically simple method offers a robust way to confine GUVs and dynamically control their outer medium, thus constituting an ideal platform to study the spatio-temporal response of reconstituted membranes and/or synthetic cell studies subjected to dynamic micro-environments.

Polydimethylsiloxane

10.26434/chemrxiv-2023-prlgn

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Optical and electrochemical properties of soluble donor-π-donor compounds as potential molecular wires and electrochemically-triggered optical switches

Physical Chemistry Chemical Physics (2003)

Laurent Porrès Valérie Alain Laurent Thouin Philippe Hapiot Mireille Blanchard‐Desce

With the aim of designing molecular wires operating at easily accessible potentials, a series of soluble bis-donor polyenic compounds (up to 3.2 nm long) has been prepared. Their optical and electrochemical properties have been investigated. These model compounds were shown to exhibit a reversible two-electron oxidation process at low potential (which further decreases with increasing polyenic chain length). The oxidized dicationic species combine convenient stability and solubility. Interestingly, these species also show reduced bond length alternation along the polyenic chain, consistent with a significant red-shift of their absorption band to the NIR region. Such behaviour is expected to lead to a dramatic enhancement of the third-order nonlinear optical responses of the polyenic molecules upon oxidation, opening the way to optical switches. Hence, such molecules represent promising model compounds for molecular wires but also open an innovative route towards voltage-driven optical switches.

Molecular switch

Molecular wire

10.1039/b305737b

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Citations (12)

Détection Electrochimique du Contenu de Gouttes Générées à l’Intérieur d’un Canal Microfluidique en Ecoulement Diphasique

Thomas Abadie C. Sella Laurent Thouin

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Formation of copper indium diselenide by electrodeposition

Journal of Electroanalytical Chemistry (1994)

Laurent Thouin Sylvie Massaccesi Sylvie Sanchez Jacques Vedel

Diselenide

10.1016/0022-0728(94)03352-8

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Citations (80)

Cyclic voltammetric studies of copper complexes catalyzing atom transfer radical polymerization

Macromolecular Chemistry and Physics (2000)

Jian Qiu Krzysztof Matyjaszewski Laurent Thouin Christian Amatore

Several copper complexes used successfully as the catalysts for atom transfer radical polymerization (ATRP) were studied by cyclic voltammetry in acetonitrile. These complexes include CuBr or CuCl complexed by 2,2′-bipyridine (bpy), 4,4′-di(5-nonyl)-2,2′-bipyridine (dNbpy), 4,4′-di(n-nonyl)-2,2′-bipyridine (dnNbpy), N,N,-N ′,N ″,N″-pentamethyldiethylenetriamine (PMDETA), N,- N-bis(2-pyridylmethyl)octylamine (BPMOA), N,N-bis(2-pyridylmethyl)octadecylamine (BPMODA), tris[(2-pyridyl)methyl]amine (TPMA) and tris[2-(dimethylamino)ethyl]amine (Me6TREN), respectively. It was found that the E1/2 value for the redox couple CuI/CuII strongly depends on the nature of the ligand and the halogen. As the number of coordination sites of the ligand increases, the reducing power of the corresponding Cu(I) complex also increases. With the same ligand, CuCl complexes usually have a lower redox potential than CuBr complexes. These general trends agree with the kinetic features of ATRP catalyzed by the corresponding complex, and a correlation between the redox potential and the apparent equilibrium constant of ATRP for methacrylate was observed. The possibility of using electrochemistry as a screening method for selecting ATRP catalysts is therefore further discussed.

Atom-transfer radical-polymerization

Bipyridine

10.1002/1521-3935(20000901)201:14<1625::aid-macp1625>3.0.co;2-9

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Citations (214)

Potential measurements in steady state voltammetry at low electrolyte/analyte concentration ratios. Role of convection on ohmic drop: a simplified model

Journal of Electroanalytical Chemistry (1998)

M. Fátima Bento Laurent Thouin Christian Amatore

Ohmic contact

Supporting electrolyte

Diffusion layer

Diffusion current

10.1016/s0022-0728(97)00477-4

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Citations (29)

Highlighting the operating regimes of microchannel electrodes under laminar flow: Mapping of photoluminescence and electrochemiluminescence through semi-transparent electrodes

Electrochemistry Communications (2024)

Yumeng Ma C. Sella Thomas Delahaye Laurent Thouin

Semi-transparent platinum electrodes were designed and optimized to implement photoluminescence (PL) and electrochemiluminescence (ECL) in microchannels under flow conditions. The luminescence properties of (2,2′-bipyridyl) ruthenium (II) were used to map steady-state emitted light through semi-transparent microchannel electrodes. Several diffusive-convective regimes were thus imposed in order to experimentally highlight the operating conditions of the electrodes, in particular the active zones at their upstream edges contributing to the Faradaic current. PL and ECL profiles were established on the electrode surfaces as a function of flow rate. The PL profiles confirmed the control of the process by mass transport. The data were validated by numerical simulations within the limits of experimental accuracy. ECL emission in presence of the co-reactant tripropylamine (TPA) was also limited by mass transport. However, in comparison the characteristics of the ECL profiles demonstrated the complexity of the underlying mechanism involving Ru (II) regeneration and TPA consumption.

Electrochemiluminescence

Microchannel

10.1016/j.elecom.2024.107759

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Citations (2)