QI Feng-chun

Changchun Institute of Biological Products

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Zheng Quanli

Xue XiangGuang

Han-hua Fang

China University of Geosciences

Gui YanPing

Xuexun Fang

Jilin University

Shao-Hong Yan

Inner Mongolia Comprehensive Disease Prevention and Control Center

Yu Wang

Northwestern Polytechnical University

Ping‐Yu Chen

National Chung Hsing University

Hao Wu

Jilin University

He Rong

Nanjing Medical University

Cooperative Institutions

Chinese Academy of Sciences

Chinese Center For Disease Control and Prevention

Jilin University

Shanghai Jiao Tong University

National Institutes for Food and Drug Control

Lanzhou University

Nanjing Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Sichuan University

Center for Disease Control

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Safety and immunogenicity of 2009 pandemic influenza A H1N1 vaccines in China: a multicentre, double-blind, randomised, placebo-controlled trial

The Lancet (2009)

Xiaofeng Liang Huaqing Wang Junzhi Wang Han-hua Fang Jiang Wu

Pandemic

10.1016/s0140-6736(09)62003-1

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Evaluation of safety of rabies vaccine prepared by degradation of Vero cell DNA with Benzonase in animals.

Zhongguo shengwuzhipinxue zazhi (2014)

QI Feng-chun Gui YanPing He Rong Jiali Zhang Huang Ming

Vero cell

Rabies vaccine

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Comparison of Influenza Virus Titers Cultured in Vero and MDCK Cells

Zhongguo shengwuzhipinxue zazhi (2006)

QI Feng-chun

Objective To explore the feasibility of culture of influenza virus type A1 in Vero and MDCK cells and optimize the culture condition.Methods Inoculate influenza virus type A1 into Vero and MDCK cells and culture in media containing fetal bovine serum(FBS) or trypsin at different concentrations.Harvest the virus liquid at different time for determination of HA titers.Results FBS inhibited the proliferation of influenza virus significantly.The addition of about 5 μg/ml of trypsin increased the virus yield.The optimal time for harvesting virus was 72-96 h after cultivation.The HA titer of influenza virus in MDCK cells was significantly higher than that in Vero cells.Conclusion Both Vero and MDCK cells were suitable for culture of influenza virus and development of influenza vaccine.

Vero cell

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Lysis of Influenza Virus by Sodium Deoxycholate

Zhongguo shengwuzhipinxue zazhi (2003)

QI Feng-chun Wang Minwen Bo Hong

Objective To determine the optimal condition of lysis of influenza virus by sodium deoxycholate.Methods Lyse infulenza virus by the sodium deoxycholate at different concentrations and purify by sucrose density gradient centrifugation.Observe the lysis of virus by electron microscopy and detect virus titers by hemagglutination reaction.Results Electron microscopy and SDS PAGE proved that the virus particles were completely lysed,and about 80% of its titer was remained.Conclusion The optimal concentration of sodium deoxycholate is 0.8%,and the optimal reaction time is 120 minutes.

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Evaluation of the anti-neuraminidase activity of the traditional Chinese medicines and determination of the anti-influenza A virus effects of the neuraminidase inhibitory TCMs in vitro and in vivo

Journal of Ethnopharmacology (2011)

Li Tian Zhiyong Wang Hao Wu Song Wang Ye Wang

Cytopathic effect

10.1016/j.jep.2011.06.002

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Citations (59)

The Study on Magnetic Field of Open Electromagnet

QI Feng-chun

Objective: Studied magnetic fied of end on open electromagnet. Got expect result. Magnetic field of D·C· electromagnet is 175.0 mT. Magnetic field of A·C· electromaagnet is 150.0mT. Magnetic field of electromagnet on all period rectification is 170.0mT. And magnetic field of music is 60.0～120.0mT.

Electromagnet

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Development and application of ELISA method for rabies virus antigen.

Zhongguo shengwuzhipinxue zazhi (2009)

Yajun Wang QI Feng-chun Xue XiangGuang

Objective To develop and preliminarily apply an ELISA method for rabies virus antigen.Methods Polyclonal antibody was prepared by immunizing rabbits with purified rabies virus CTN-1V antigen.A double antibody sandwich ELISA method was developed by using the prepared polyclonal antibody as labeling antibody and purified horse antisera against rabies as coating an-tibody,then verified and used for determination of virulence of virus bulk and potency of vaccine.The determination result was com-pared with that by NIH test.Results The minimum detection limit,optimal linear range and correlation coefficient of the developed ELISA method were 0.17 IU / ml,0.17 ~ 2.00 IU / ml and more than 0.97 respectively,and both inter-and intra-variation coeffi-cients were less than 10%.The developed ELISA method showed good specificity and stability.The determination results of 20 batches of virus bulks and prepared vaccines by the developed method showed no significant differences with those by NIH method.Conclusion An ELISA method for rabies virus antigen is developed,which may be used for the control tests on bulk and final bulk during production of rabies vaccine.

Polyclonal antibodies

Rabies vaccine

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Construction of Hepatitis B DNA Vaccine and Immune Response in Mice Induced by the Vaccine

The Journal of Microbiology (2004)

Zhao Da-peng WU Xiao-juan Yan Zhang Mei Leng QI Feng-chun

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Safety and Immunogenicity of Domestic Split-virion Influenza Vaccine

China Modern Doctor (2010)

QI Feng-chun

Objective To evaluate the safety and immunogenicity of the domestic split-virion influenza vaccine.Methods An open designed clinical trial was performed in 322 subjects in Jianhu of Yancheng city,Jiangsu Province to investigate its safety, seroconversion rate,geometric mean titer (GMT) and seroprotection rate.Results After immunization,the systemic and local reaction rates were 4.52% and 0.6%.319 subjects were assessed in immunogenicity after vaccination.The anti-HI seroconversion rates of H1N1, H3N2 and B hypotype were 94.98%,87.1% and 88.1%,respectively.The anti-HI GMT showed 33.5-time.7.8-time.and 26.2-time.increase,respectively.The anti-HI seroprotection rates were 100% ,99.7% and 98.4% respectively.Conclusion The domestic split-virion influenza vaccine has excellent safety and immunogenicity,and it is suitable for wide use.

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[Evaluation on the safety and immunogenicity of split influenza virus vaccine].

PubMed (2009)

Zhang Xue-mei QI Feng-chun Chunyi Wang

To evaluate the safety and immunogenicity of split influenza virus vaccine made in changchun institute of biological products.Cluster samples were selected by random to carry out the single center, open clinical trial.After one dose injection, the rate of local reaction were 0.6%, and fever rate was 4.52%. The majority of the people with fever were mild fever. The HI Antibody positive rate of Influenza H1N1, H3N2, and B type were 94.98%, 87.1% and 88.1%; Geometric mean titer (GMT) of the three types antibody increased 104.79, 69.97 and 40.83 times respectively. The antibody protective rate were 100%, 99.7%, and 98.4% respectively. There were statistical difference on the HI antibody positive rate, times of GMT increased, and the antibody protective rate among the three types of influenza vaccine.It is safety of split influenza virus vaccine for the people who are > or = 3-years-old, which made in changchun institute of biological products, and with good immunogenicity.

Antibody titer

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