<p class="ABNT-Resumoeabreviaturas"><span>A Vila de Jericoacoara, localizada no município de Jijoca de Jericoacoara, Ceará, e a estação de tratamento de esgoto (ETE) local estão situadas próximas ao Parque Nacional de Jericoacoara. O esgoto tratado pela ETE é descarregado no solo arenoso para percolação, o que pode resultar em contaminação do solo e da água subterrânea. Durante a estação chuvosa, a saturação do solo dificulta a percolação do efluente tratado, fazendo com que este adentre o parque e forme um curso d'água intermitente que, ao se unir a outros fluxos, deságua no mar após atravessar a área posterior à Duna do Pôr do Sol, um ponto turístico local. Para avaliar a qualidade microbiológica do efluente que adentra o parque, amostras de água foram coletadas ao longo de seis meses, abrangendo parte das estações chuvosa e seca, em três pontos a jusante. Foram realizadas análises físico-químicas de pH e condutividade elétrica em quinze amostras de água, além de análises microbiológicas utilizando a técnica de fermentação em tubos múltiplos para determinar o número mais provável (NMP) de coliformes totais e termotolerantes. Os resultados indicaram que os valores de pH e condutividade variaram conforme o volume de chuvas na região. Os dados microbiológicos apontaram para a presença de mais de 2,0 × 10<sup>6</sup> NMP de coliformes termotolerantes por 100 mL de água efluente, tanto na estação seca quanto na chuvosa, sugerindo que a ETE é uma fonte significativa de contaminação microbiológica no parque. Durante a estação chuvosa, essa contaminação tende a se disseminar na unidade de conservação devido à saturação do solo e à formação de cursos d'água. A presença de contaminação microbiológica representa riscos de infecções microbianas e parasitárias para visitantes e espécies locais, além de comprometer a função legal da unidade de conservação federal. Este estudo é pioneiro ao demonstrar a ocorrência de contaminação microbiológica na área do Parque Nacional de Jericoacoara.</span></p>
Effects of nutritional and physicochemical parameters on the production of biosurfactants by Bacillus subtilis RI4914 were evaluated. Effects of three different concentrations of NH4NO3 and/or yeast extract were tested. The treatment with 2.0 g l−1 of NH4NO3 without yeast extract resulted in the highest biosurfactant, organic acid, solvent and polymer concentrations, and this cultivation condition was selected for the residual oil recovery experiments. The lower interfacial tension between crude oil and the culture supernatant was 0.07 mN/m and this result was achieved at NaCl concentrations up to 120 g l−1. Culture fluid with 600 mg l−1 biosurfactant and the polymer produced by the bacterium resulted in residual oil recoveries up to 88%. The attainment of low interfacial tensions and high oil recoveries with the biosurfactant concentrations used at high salinity and temperature indicated that B. subtilis RI4914 is an excellent candidate for application in MEOR.
Here, we report the draft genome sequence and annotation of Nocardia farcinica TRH1, a petroleum hydrocarbons degrading Actinobacteria isolated from the coastal water of Trindade Island, Brazil.
Aiming to record and analyze the prevalence of infestation by ciliated protozoa peritrichs colonizing oligochaetes along the Paraibuna River, Juiz de Fora City, Minas Gerais, a sampling of sediment was performed at different sampling stations along the Paraibuna River. The oligochaetes were screened from the fixed samples, and then examined through light microscopy, to quantify the prevalence of infestation of epibiont ciliates. Several physicochemical parameters of water were analized in all sampling sites. Rhabdostyla sp. was found only in three of the five collecting points and greater abundance of oligochaetes in some sites may be related to the preference of both peritrichs ciliates and oligochaetes for eutrophic environments, due to the fact that these points were already located in urbanized locations. The occurrence and the prevalence of epibiont pertrichs have been identified as an important tool for evaluanting water quality in lotic systems. The data suggest a direct relation between prevalence of infestation and organic pollution, that can be a direct contributor to the spread of several diseases among people who may have contact with the water of this environment.
In silico analysis can be useful to infer about the horizontal gene transfer (HGT) as well as to deduce about the evolutionary relations of catabolic genes.In this study, we performed the analysis of two housekeeping genes (fabD and rpoD) and two catabolic genes (alkB and catA) from 12 bacterial genus usually founded in marine environments.Comparing the trees obtained from Bayesian Inference hypotheses of these genes with 16S rDNA sequences, we noted the topologies are different among housekeeping or catabolic genes trees comparing to 16S gene tree.The HGT may be used with the purpose to spread genes within bacterial community according to environmental conditions in marine ecosystems.In this way, using our analysis, we concluded that hydrocarbons catabolism genes as well as housekeeping genes can be subject to horizontal gene transfers among marine bacterial communities.
ABSTRACT Here, we present a draft genome and annotation of Rhodococcus rhodochrous TRN7, isolated from Trindade Island, Brazil, which will provide genetic data to benefit the understanding of its metabolism.
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