Douglas E. Lorenz

University of Vermont

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

John C. Petricciani

International Alliance for Biological Standardization

Hope E. Hopps

Center for Biologics Evaluation and Research

Robert H. Purcell

Sport Ireland

Paul Albrecht

Heinrich Heine University Düsseldorf

Jules L. Dienstag

Massachusetts General Hospital

Robert J. Gerety

Biogen (United States)

Lewellys F. Barker

Aeras

J Drucker

Institut de Veille Sanitaire

L. F. Barker

Binus University

P. J. Vasington

Armed Forces Institute of Pathology

Cooperative Institutions

National Institutes of Health

Center for Biologics Evaluation and Research

National Institute of Allergy and Infectious Diseases

United States Food and Drug Administration

National Cancer Institute

Food and Drug Administration

American Red Cross

Johns Hopkins University

National Institute of Diabetes and Digestive and Kidney Diseases

University of California, Los Angeles

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Response : Human Virus Vaccines: Why Monkey Cells?

Science (1972)

John C. Petricciani Hope E. Hopps Douglas E. Lorenz

Haplorhini

10.1126/science.176.4036.814-a

Cite

Citations (0)

On the production of highly labeled tumor virus deoxyribonucleic acid.

Proceedings of the National Academy of Sciences (1967)

Samuel W. Luborsky Douglas E. Lorenz David L. Reynolds John E. Verna Earl Usdin

10.1073/pnas.57.5.1286

Cite

Citations (1)

Encephalitogenicity of measles virus in marmosets

Infection and Immunity (1981)

Paul Albrecht Douglas E. Lorenz M J Klutch

Marmosets infected intracerebrally with the wild Edmonston strain of measles virus developed encephalitis, demonstrated histologically and by the fluorescent-antibody technique. The infection remained clinically silent over a 14-day observation period. Animals infected intracerebrally with the JM strain of wild measles virus had only mild encephalitic changes but died of the visceral form of measles infection. Marmosets inoculated with measles vaccine had no encephalitis and remained clinically well. Marmosets appear to be a sensitive indicator of the viscerotropic and neurotropic properties of measles virus.

Morbillivirus

10.1128/iai.34.2.581-587.1981

Cite

Citations (21)

Review of infectivity studies in nonhuman primates with virus-like particles associated with MS-1 hepatitis

The American Journal of the Medical Sciences (1975)

James E. Maynard Douglas E. Lorenz Daniel W. Bradley S M Feinstone D H Krushak

Infectivity

Acute hepatitis

10.1097/00000441-197507000-00012

Cite

Citations (42)

Hepatitis in the Marmoset, Saguinus mystax

Experimental Biology and Medicine (1970)

Douglas E. Lorenz L. F. Barker Daniel Stevens M W Peterson Ruth L. Kirschstein

The marmoset, Saguinus mystax, developed signs of disease similar to human hepatitis when inoculated with marmoset sera containing Deinhardt's hepatitis agent, or sera from patients with infectious hepatitis but not when inoculated with serum containing hepatitis-associated antigen or with partially purified antigen. The evidence of disease were 3- to 10-fold increase above normal SGPT levels and histologic changes of single liver cell necroses with periportal lymphocytic infiltration. Hepatitis-associated antigen was not found in any marmoset sera by complement-fixation tests or electron microscopy.

Complement fixation test

10.3181/00379727-135-35049

Cite

Citations (40)

Virus-like particles and GB agent hepatitis

Nature (1976)

Jules L. Dienstag Jo Ann Wagner Robert H. Purcell William T. London Douglas E. Lorenz

10.1038/264260a0

Cite

Citations (14)

Hepatitis a Antigen Isolated from Liver and Stool: Immunologic Comparison of Antisera Prepared in Guinea Pigs

The Journal of Immunology (1976)

Jules L. Dienstag Alan N. Schulman Robert J. Gerety Jay H. Hoofnagle Douglas E. Lorenz

Abstract Morphologically similar hepatitis A antigen particles (HA Ag)3 have been detected in the stools of patients with type A hepatitis and in the livers of marmosets experimentally infected with hepatitis A virus. To investigate the humoral antibody responses to these antigens and to compare the immunologic properties of HA Ag from these two sources, we immunized guinea pigs with either marmoset liver-derived HA Ag or with human stool-derived HA Ag in complete Freund's adjuvant and measured their antibody responses by immune electron microscopy (IEM) and immune adherence hemagglutination (IAHA). Antibodies reacting with both hepatitis A antigens were elicited in both groups. As determined by IEM, no distinction was seen between the reaction of guinea pig antiserum to each HA Ag tested under code when reacted against either liver-derived or stool-derived HA Ag. Antibodies elicited to marmoset liver-derived HA Ag and human stool-derived HA Ag had similar end point dilution titers by IAHA when tested against either “light” density (1.34 g/cm3) or “heavy” density (1.40 g/cm3) stool-derived HA Ag or liver-derived HA Ag. Low levels of antibody to normal liver or stool control antigens were observed transiently but did not obscure the specific response to HA Ag. These data suggest that morphologically similar HA Ag particles from different sources and with different densities are immunologically similar and may be identical. In contrast to the heterogeneity of surface antigens of hepatitis B virus, the comparable immunogenicity and apparent antigenic homogeneity of HA Ags derived from various sources may simplify the approach to development of a vaccine against viral hepatitis, type A.

Immune adherence

10.4049/jimmunol.117.3.876

Cite

Citations (17)

Localization of Hepatitis A Antigen in Marmoset Organs during Acute Infection with Hepatitis A Virus

The Journal of Infectious Diseases (1978)

Peter M. H. Heegaard J Drucker Douglas E. Lorenz J. Wagner R. J. Gerety

Twelve marmosets (Saguinus mystax) were inoculated intravenously (iv) with hepatitis A virus (HAV). One died early (day 12); seven were sacrificed at the time of elevation in level of alanine aminotransferase (serum glutamic-pyruvic transaminase), and four without elevation were not sacrificed but seroconverted. In the seven marmosets sacrificed during the acute stage of illness, hepatitis A antigen (HA Ag) was detected in the liver by immunofluorescence in all cases, by immune electron microscopy in four, and by enzyme-linked immunosorbent assay (ELISA) in three. The HA Ag appeared by immunofluorescence as very fine granules in the cytoplasm of hepatocytes and Kupffer cells. The HA Ag could not be detected by immunofluorescence in biopsy specimens taken from the duodenum, jejunum, ileum, or transverse colon in any of eight marmosets in which necropsy was performed during the acute or preacute stage of illness. These findings suggest that the gut is not involved during the acute phase of HAV infection following iv inoculation into marmosets. The ELISA results showed that only three of 12 marmoset livers obtained during the acute phase of HAV infection could be used as an antigen source in serologic testing for antibody to HA Ag. Thus, marmoset livers were no better as a source of HA Ag than acute-phase stools from patients with type A hepatitis.

Immunofluorescence

10.1093/infdis/138.3.369

Cite

Citations (95)

Use-Dilution Test and Newcastle Disease Virus

Applied Microbiology (1964)

Douglas E. Lorenz Gregory J. Jann

The use-dilution test for evaluating the effectiveness of disinfectants against bacteria was modified to determine the effectiveness of disinfectants against a group of viruses. Modifications were kept to a minimum to retain the general principles of the test and thereby retain the test's familiarity among testing laboratory personnel. Modifications included the use of a standard allantoic fluid suspension of Newcastle disease virus instead of a standard bacterial culture. The only other modification was the inoculation of six embryonated chicken eggs (10 to 12 days old) with 0.1 ml of nutrient broth into which a carrier ring was transferred after a standard period in diluted disinfectant. The death or survival of 60 embryos, then, is the criterion by which a disinfectant can be judged effective at use-dilution. Experiments are described which establish the validity of the modified test procedure. The effectiveness of nine common disinfectants against Newcastle disease virus as judged by this test procedure is reported.

Embryonated

Disinfectant

Newcastle Disease

Dilution

Serial dilution

10.1128/aem.12.1.24-26.1964

Cite

Citations (8)

Development and characerization of cell lines from subhuman primates

In Vitro (1973)

Roslyn E. Wallace P. J. Vasington John C. Petricciani Hope E. Hopps Douglas E. Lorenz

Senescence

African Green Monkey

10.1007/bf02619057

Cite

Citations (57)