Qi Huo

Xinjiang Medical University

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Cong-gai Huang

Affiliated Hospital of Southwest Medical University

Shutao Zheng

First Affiliated Hospital of Xinjiang Medical University

Renyong Lin

First Affiliated Hospital of Xinjiang Medical University

Ilyar Sheyhidin

First Affiliated Hospital of Xinjiang Medical University

Xiaomei Lu

First Affiliated Hospital of Xinjiang Medical University

Aerziguli Tuerxun

Xinjiang Medical University

Jun-guo Feng

Xinjiang Medical University

Xing Wang

Southeast University

Qing Liu

Central South University

Qing Liu

Shandong University

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Xinjiang Medical University

First Affiliated Hospital of Xinjiang Medical University

Xinjiang Uygur Autonomous Region Disease Prevention and Control Center

Nanjing Medical University

Sichuan University

Sichuan Agricultural University

Tumor Hospital of Xinjiang Medical University

Xinjiang Uygur Autonomous Region Education Department

Dongguan People’s Hospital

Clinical Research Institute

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[Expression of ERK1/2 MAPK signaling transduction pathway in esophageal cancers in Kazakh patients].

Zhonghua zhongliu zazhi (2011)

Shutao Zheng Tao Liu Aerziguli Middottuersun Qi Huo Qing Liu

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Effection of short hairpin RNA targeting extracellular signal-regulated kinase 2 on proliferation of Eca109 cells

Zhonghua shiyan waike zazhi (2010)

Qi Huo Shutao Zheng Aerziguli Tuerxun Qing Liu Jun-guo Feng

Objective To study the effect of short hairpin RNA (shRNA) targeting extracellular signal-regulated kinase 2 (ERK2) on proliferation of human esophageal cancer cell line Eca109. Methods The recombinant plasmid (pGeneClipTM-shRNA-ERK2) was constructed and transfected into the human esophageal cancer cell line Eca109 by liposome, and the transfection efficiency was observed by reverse fluorescence microscope. The proliferation ability of esophageal cancer Eca109 cells after transfection was analyzed by methyl thiazolyl tetrazolium (MTF). The expression of ERK2 and inhibitor apoptosis gene Survivin following transfection was detected by Western blotting. Results Sequencing proved that recombinant plasmid was successfully constructed, and the transfection efficiency was about 50%-70% after 72 h following transfection. The Eca109 cell growth at 96 h after transfection was significantly inhibited by 10.45% as compared with negative group and blank group,P ＜0. 05. The protein expression of ERK2 and Survivin was decreased in transfection group as compared with the U0126 group and negative group at 72 h and 96 h ( P ＜ 0. 05 ). Conclusion The recombinant plasmid ( pGeneClipTM-shRNA-ERK2) has been proved not only to be effective for down-regulation of ERK2, but also can affect the expression of Survivin and significantly inhibit the Eca109 cells proliferation. Key words: Esophageal carcinoma; Small interference; Extracellular signal-regulated kinase; Cell proliferation

Survivin

10.3760/cma.j.issn.1001-9030.2010.09.004

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Relationship between esophageal cancer and genetic polymorphisms of O~6-methylguanine-DNA methyltransferase gene

Zhonghua shiyan waike zazhi (2009)

Liang Yu Guodong Lv Wei-jing Ma Qi Huo

Objective To explore relationship between esophageal cancer and genetic polymor-phisms of 06-methylguanine-DNA methyltransferase (MGMT) gene in Kazakh in Xinjiang. Methods The 5 SNPs of MGMT gene in 51 esophageal squamous cell cancer (ESCC) patients and 109 controls of Kazakh in Xinjiang,were genotyped by PCR-RFLP based on ease-control study strategy. Results Logistic regression analysis showed that the proportion of allele for promoter 485C > A in ESCC group was different from that in control group (P A of MGMT gene was associated with ESCC, and the rest 4 SNPs may be not with ESCC. The 5 SNPs of MGMT gene may jointly poly a role in the susceptibility of ESCC in Kazakh in Xinjiang,and allele variants increased risk of the occurrence of ESCC. Key words: Esophageal carcinoma; MGMT; SNP; Kazakh

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10.3760/cma.j.issn.1001-9030.2009.11.013

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shRNA interference for extracellular signal-regulated kinase 2 can inhibit the growth of esophageal cancer cell line Eca109

Journal of Receptors and Signal Transduction (2010)

Qi Huo Shutao Zheng Aerziguli Tuersun Cong-gai Huang Qing Liu

Background: Esophageal squamous cell carcinoma is one of the most common digestive tract cancers with 5-year survival rate less than 10% owing to its poor prognosis. Mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling pathway has been mainly involved in the pathogenesis of various cancers. In present study, we investigated the role of ERK2 in human esophageal cancer cell line Eca109.Methods: Short-hairpin RNA (shRNA) interference vector targeted ERK2 was constructed using pGeneclip U1 hairpin cloning systems, then transfected into Eca109 cell line. The transfection efficiency was observed by fluorescence microscope and cell growth after transfection with shRNA-ERK2 vector was determined by methylthiazolyl blue tetrazolium (MTT) assay. The ERK2 expression after transfection was detected by western-blotting. The cell apoptosis and cell-cycle was analyzed by flow cytometry. The role of p-ERK2 was confirmed by immunohistochemistry and soft agar colony formation assay.Results: The growth of Eca109 transfected with shRNA-ERK2 vector was obviously inhibited compared to control group via MTT analysis. The inhibition rate after transfection with shRNA-ERK2 for 96 h was 10.45%, the expression of ERK2 was obviously reduced compared to the control analyzed by western-blot, cell apoptosis was 9.7% (compared to control, P < 0.05), and cell-cycle was arrested at G1 phase.Conclusions: In present study we demonstrated for the first time that transfection with shRNA-ERK2 targeted ERK2 into Eca109 cells can inhibit growth of Eca109, inducing cell apoptosis and influencing cell-cycle. Together, these results we obtained suggested that ERK2 plays an important role in cell growth of Eca109.

MTT assay

10.3109/10799891003786200

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The expression and activation of ERK/MAPK pathway in human esophageal cancer cell line EC9706

Molecular Biology Reports (2010)

Shutao Zheng Qi Huo Aerziguli Tuerxun Wenjing Ma Guo-dong Lv

10.1007/s11033-010-0178-z

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Citations (19)