Ana Huertas-López

Universidad de Murcia

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

GÁ

Gema Álvarez‐García

Universidad Complutense de Madrid

Carlos Martínez‐Carrasco

Universidad de Murcia

J. M. Castro Cerón

Universidad de Murcia

Silvia Martínez‐Subiela

Universidad de Murcia

Ignacio Ferre

Universidad Complutense de Madrid

Roberto Sánchez-Sánchez

Universidad Complutense de Madrid

Luís Miguel Ortega-Mora

Universidad Complutense de Madrid

Carlos Diezma‐Díaz

Universidad Complutense de Madrid

Woraporn Sukhumavasi

Chulalongkorn University

Nadia María López-Ureña

Universidad Complutense de Madrid

Cooperative Institutions

Universidad Complutense de Madrid

Instituto de Salud Carlos III

Universidad de Murcia

Universitat Autònoma de Barcelona

University of Washington

Seattle University

Instituto de Investigación en Recursos Cinegéticos

Consejo Superior de Investigaciones Científicas

Cambridge University Press

University of Córdoba

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

A time-resolved fluorescence immunoassay for the detection of anti-Neospora caninum antibodies in sheep

Veterinary Parasitology (2019)

Ana Huertas-López Carlos Martínez‐Carrasco J. M. Castro Cerón Roberto Sánchez-Sánchez Ángela Vázquez-Calvo

Neospora

10.1016/j.vetpar.2019.108994

Cite

Citations (8)

Seronegativity of Iberian ibex (Capra pyrenaica) against Toxoplasma gondii and Neospora caninum is consistent with eco-epidemiological and environmental features in Mediterranean mountainous areas

Research in Veterinary Science (2025)

Alejandro Cano-Manuel José Enrique Granados Gema Álvarez‐García Ana Huertas-López Carlos Diezma‐Díaz

Seroprevalence

Zoonosis

Toxoplasmosis

10.1016/j.rvsc.2025.105530

Cite

Citations (0)

Development and validation of a time-resolved fluorescence immunoassay for the detection of anti-Toxoplasma gondii antibodies in goats

Veterinary Parasitology (2021)

Ana Huertas-López Silvia Martínez‐Subiela J. M. Castro Cerón Ángela Vázquez-Calvo Elvis Danilo Pazmiño-Bonilla

Toxoplasmosis

10.1016/j.vetpar.2021.109432

Cite

Citations (12)

Seroprevalence of Toxoplasma gondii in outdoor dogs and cats in Bangkok, Thailand

Parasitology (2021)

Ana Huertas-López Woraporn Sukhumavasi Gema Álvarez‐García Silvia Martínez‐Subiela David Cano‐Terriza

Abstract The aim of this study was to estimate the seroprevalence and risk factors associated with Toxoplasma gondii exposure in dogs and cats from Bangkok, Thailand. Blood samples from 318 dogs and 321 cats were tested for T. gondii antibodies by modified agglutination test (cut-off 1:25). Additionally, 18 dogs and 20 cats were longitudinally sampled for T. gondii antibodies during the same study period, between June and July 2019. The overall seroprevalence in dogs and cats was 7.9% (25/318; 95% CI 4.9–10.8%) and 18.7% (95% CI 14.4–23.0%), respectively. For dogs, risk factors identified were being a mixed-breed animal and living totally outdoors, while increasing age was shown to be a risk factor for cats. Seroconversion was not detected and titres from positive animals remained constant over longitudinal study. The present study indicates that there is a prominent presence of T. gondii in urban and peri-urban areas of Bangkok, suggesting that outdoor dogs and cats should be considered as a possible risk factor for humans.

Seroprevalence

Seroconversion

Direct agglutination test

Toxoplasmosis

10.1017/s0031182021000421

Cite

Citations (15)

Transcriptomics of Besnoitia besnoiti-Infected Fibroblasts Reveals Hallmarks of Early Fibrosis and Cancer Progression

Microorganisms (2024)

María Fernández-Álvarez Pilar Horcajo Alejandro Jiménez‐Meléndez Pablo Angulo Lara Ana Huertas-López

Endothelial injury, inflammatory infiltrate and fibrosis are the predominant lesions in the testis of bulls with besnoitiosis that may result in sterility. Moreover, fibroblasts, which are key players in fibrosis, are parasite target cells in a Besnoitia besnoiti chronic infection. This study aimed to decipher the molecular basis that underlies a drift toward fibrosis during the disease progression. Transcriptomic analysis was developed at two times post-infection (p.i.), representative of invasion (12 h p.i.) and intracellular proliferation (32 h p.i.), in primary bovine aorta fibroblasts infected with B. besnoiti tachyzoites. Once the enriched host pathways were identified, we studied the expression of selected differentially expressed genes (DEGs) in the scrotal skin of sterile infected bulls. Functional enrichment analyses of DEGs revealed shared hallmarks of cancer and early fibrosis. Biomarkers of inflammation, angiogenesis, cancer, and MAPK signaling stood out at 12 h p.i. At 32 h p.i., again MAPK and cancer pathways were enriched together with the PI3K–AKT pathway related to cell proliferation. Some DEGs were also regulated in the skin samples of naturally infected bulls (PLAUR, TGFβ1, FOSB). We have identified potential biomarkers and host pathways regulated during fibrosis that may hold prognostic significance and could emerge as potential therapeutic targets.

10.3390/microorganisms12030586

Cite

Citations (0)

A systematic review and meta-analysis of the serological diagnosis of Toxoplasma gondii infection highlight the lack of a One Health integrative research

Research in Veterinary Science (2023)

Ana Huertas-López Gema Álvarez‐García Roberto Sánchez-Sánchez Ana Cantos-Barreda Francisco Javier Ibáñez-López

Toxoplasma gondii is a globally distributed food-borne zoonotic parasite with numerous infection sources. The control of this zoonosis requires a One Health response that partially depends on serological monitoring in humans and animals. Herein, a systematic review and a meta-analysis were performed to analyse and compare the transdisciplinary and integrative research under the One Health approach. We searched for articles published between January 1st 2014 and September 5th 2022, focused on the development and evaluation of serological techniques for the diagnosis of T. gondii infection in humans and animals. After an exhaustive search on three scientific databases, a quality assessment was performed on 291 articles by QUADAS-2 tool, and 113 articles were finally selected. A total of 18 variables were extracted and analysed, including bibliometric characteristics, study aims and methodology. Remarkably, none of the studies included in the meta-analysis explicitly quoted the words "One Health", and only 23.9% of them alluded to the principles underlying the One Health approach; in particular, none were conducted by physician-only teams, with the majority of these studies involving interdisciplinary research teams, followed by veterinarians and by non-physician or non-veterinarian researchers. The One Health approach followed in the serodiagnosis of T. gondii still needs further integration among scientific disciplines, which is essential to design effective control strategies.

Zoonosis

One Health

10.1016/j.rvsc.2023.01.005

Cite

Citations (13)

Fasciolosis in Grazing Cattle

Ana Huertas-López Ignacio Ferre

Fasciolosis

Cattle grazing

10.1007/978-3-031-52133-1_38-1

Cite

Citations (0)

Dictyocaulosis in Cattle

Ana Huertas-López Gema Álvarez‐García

10.1007/978-3-031-52133-1_283-1

Cite

Citations (0)

Treatment with BKI-1748 after Toxoplasma gondii systemic dissemination in experimentally infected pregnant sheep improves fetal and lamb mortality and morbidity and prevents congenital infection

Antimicrobial Agents and Chemotherapy (2024)

Roberto Sánchez-Sánchez Ana Huertas-López Andrea Largo-de la Torre Ignacio Ferre Filippo Maria Dini

Drug development for congenital toxoplasmosis is challenging since first-line therapy has a high rate of adverse effects and exhibits suboptimal efficacy. Bumped kinase inhibitors (BKIs), targeting protein kinases with small gatekeeper residues, have been found to be effective against

Toxoplasmosis

10.1128/aac.01448-24

Cite

Citations (0)

Changes in serum biomarkers of inflammation in bovine besnoitiosis

Parasites & Vectors (2021)

David González‐Barrio Ana Huertas-López Carlos Diezma‐Díaz Ignacio Ferre J. M. Castro Cerón

Acute and chronic besnoitiosis in extensive natural-service herds can have relevant effects in the health of bulls and negative consequences in their productive performance. Recent progress has been made in order to elucidate the pathogenesis of this disease. In this context, the study of biomarkers of inflammation in serum would contribute to gaining knowledge about the physiopathology of bovine besnoitiosis. Serological biomarkers could help in early diagnosis and prognosis, as seropositive bulls may have mild or severe testicular lesions.Herein, we have investigated the diagnostic and/or prognostic value of a panel of serum (serological) biomarkers related to inflammation, including total protein, globulin and albumin, haptoglobin (Hp), adenosine deaminase (ADA) paraoxonase-1 (PON-1) and acetylcholinesterase (AChE) in naturally and experimentally B. besnoiti-infected males classified according to different clinical phases of the disease (acute, chronic and subclinical besnoitiosis).Results showed a similar response pattern in these biomarkers for naturally and experimentally infected cattle, with a few relevant variations. Most significant changes occurred during the acute phase of infection, although significant changes in a few biomarkers were also observed during the chronic infection. Haptoglobin, albumin, PON-1 and ADA were identified as the biomarkers that showed changes of higher magnitude in the acute phase of the infection, whereas high total protein and globulin values were found in chronically infected cattle. We have described the changes of a panel of inflammatory biomarkers of acute and chronic bovine besnoitiosis.In summary, several biomarkers with promising diagnostic value have been identified. The biomarkers associated with acute infection are related to previously reported molecular biomarkers in testicular parenchyma of infected bulls and could help in the diagnosis of early infections and complement results from specific immunoglobulin M (IgM) detection.

Haptoglobin

Subclinical infection

10.1186/s13071-021-04991-0

Cite

Citations (4)