Naima Ghailani

Abdelmalek Essaâdi University

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Amina Barakat

Abdelmalek Essaâdi University

Fouad Sayah

Abdelmalek Essaâdi University

Mohcine Bennani Mechita

Abdelmalek Essaâdi University

Kacem Rharrabe

Abdelmalek Essaâdi University

Fatiha Benyahya

Institut Pasteur du Maroc

Noureddin Bouayad

Abdelmalek Essaâdi University

C. L. Guillemin

Institut Agro Montpellier

Rachid Jbilou

Abdelmalek Essaâdi University

Hadba Echchaoui

Institut Pasteur du Maroc

Abdeslam Ennabili

Sidi Mohamed Ben Abdellah University

Cooperative Institutions

Abdelmalek Essaâdi University

Institut Pasteur du Maroc

Centre National de la Recherche Scientifique

Institut National de la Recherche Agronomique

Sorbonne Université

École Nationale de Commerce et de Gestion de Tanger

Association Marocaine Pour la Recherche Développement

Service Régional Vaudois de Transfusion Sanguine

Institut National de la Transfusion Sanguine

Centro de Investigaciones Biológicas Margarita Salas

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First report of APOB gene variations in Moroccans

Ibnosina Journal of Medicine and Biomedical Sciences (2012)

Fatiha Benyahya Amina Barakat Naima Ghailani Hadba Echchaoui Bouchrail Echchaoui

The normal clearance of LDL by the LDL receptor is dependent upon normal function of both the LDL receptor and of apoB-100. Accordingly, mutations in the LDL receptor gene and in the apoB-100 gene have been found to cause autosomal dominant hypercholesterolemia. Mutations in the LDL receptor gene cause familial hypercholesterolemia, whereas mutations in the apoB-100 gene cause familial defective apoB-100. In Front lack of data on genotype and phenotype among ADH patients in Moroccan populations, we carried up this study to determine genotype of ADH among subjects of north of Morocco, who have increased rates of TC, LDLc, ApoB and TG. 46 probands were studied. Genomic DNA was isolated us described previously. Direct sequencing is used to determine the mutations on genes LDLR, APOB and PCSK9 and the genotype of the apoE. We have detected for the first time in the Moroccan population three known polymorphisms and V4238A mutation combined with increased TG levels in three probands. This mutation appears to be common polymorphism. Those patients were homozygous of alleles E3/E3. In conclusion, further studies are needed to elucidate the molecular basis of the dyslipidemia observed in the studied probands.

Proband

Apolipoprotein E

10.4103/1947-489x.210765

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Study of the vesicles released during conservation of red cells.

PubMed (1995)

Naima Ghailani C. L. Guillemin C. Vigneron

The purpose of this study was to determine the protein composition of the vesicles released from senescent erythrocytes and its variation during their experimental conservation at +4 degrees C in citrate phosphate dextrose (CPD), over a period of 8 weeks. Techniques employed included electron microscopy, SDS-PAGE and immunoblotting. Electron microscopic observation of senescent erythrocytes showed the simultaneous release of one or several vesicles of varying size and shape, while close examination of individual vesicles revealed a slack membrane structure and the presence of band 3 protein. SDS-PAGE and immunoblotting showed the vesicles to be composed mainly of band 3 and its breakdown products and to be lacking in spectrin. Use of specific antibodies demonstrated the presence of free haemoglobin, immunoglobulin G (IgG) and fragment C3b of complement. During conservation for 8 weeks, the concentration of band 3 protein decreased, while the concentrations of IgG and C3b increased and there was no apparent variation in haemoglobin levels.

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Citations (2)

Effects of gamma irradiation on fourth-instar Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)

Journal of Stored Products Research (2004)

Khalid Azelmat Fouad Sayah Mohamed Mouhib Naima Ghailani Driss Elgarrouj

Galleria mellonella

Plodia interpunctella

Gamma Irradiation

10.1016/j.jspr.2004.05.003

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Citations (36)

Insecticidal effects of Moroccan plant extracts on development, energy reserves and enzymatic activities of Plodia interpunctella

Spanish Journal of Agricultural Research (2012)

Noureddin Bouayad Kacem Rharrabe Naima Ghailani Rachid Jbilou P. Castañera

The aim of this work was to study the effects of methanol extracts of ten plant species used in traditional medicine in Morocco (Peganum harmala, Ajuga iva, Rosmarinus officinalis, Lavandula stoechas, Lavandula dentata, Cistus ladanifer, Cistus salviaefolius, Cistus monspeliensis, Centaurium erythraea and Launaea arborescens) on Plodia interpunctella Hübner (Lepidoptera: Pyralidae) larvae. Firstly, we studied the effects of the ingestion of these extracts at 500 ppm on post-embryonic development parameters. Most plant extracts provoked a notable decrease of larval weight 8 days after treatment (up to 33% weight loss with C. erythraea) and caused significant alterations on pupation (ranging from 5% to 85%) and adult emergence (below 2.5% with R. officinalis, C. erythraea and A. iva). The plant extracts that showed strongest effects on post-embryonic development were selected to test their effects on the following physiological parameters: larval reserve substances (at 500 ppm); and midgut activities of hydrolytic and detoxification enzymes (at 500, 750 and 1000 ppm). All treatments provoked a significant reduction of protein and carbon hydrate larval contents, the inhibition of proteases and α-amylase activities in a dose depended manner, and the induction of glutathione S-transferase and esterase (using MtB as substrate) activities, whereas the activity of cytochrome P450 monooxygenases and esterases (using 1-NA as substrate) increase or decrease depending on the extract concentration and the plant analyzed.

Rosmarinus

Lavandula

10.5424/sjar/2013111-692-11

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Citations (33)

Screening of BRCA1/2 genes mutations and copy number variations in patients with high risk for hereditary breast and ovarian cancer syndrome (HBOC)

BMC Cancer (2020)

Fatima Zahra El Ansari Farah Jouali Nabila Marchoudi Mohcine Bennani Mechita Naima Ghailani

Abstract Background Hereditary breast and ovarian cancer (HBOC) is an autosomal dominant inherited cancer susceptibility disorder. Both BRCA1 and BRCA2 genes are considered as high penetrance genes of this syndrome. The identification of BRCA1/2 genetic alterations before cancer development, grant patients the chance to benefit from various medical cancer prevention approaches. Therefore, the appearance of recent advanced technologies in molecular analysis such as next generation sequencing has simplified full BRCA1/2 analysis. Many attempts took place in hope of understanding the molecular germline spectrum of these two genes in Moroccan HBOC patients. However, most of the past projects focused only on young breast cancer cases, lacked ovarian cancer cases in their cohort and only a limited number of these studies were able to analyze the entire exons or copy number variations for both genes. In attempt of gaining more information regarding the molecular profile of BRCA1/2 in HBOC, we conducted a study in which we analyze their molecular profile on selected Moroccan patients suspected of having HBOC syndrome. Methods In this study we obtained blood samples from 64 selected Moroccan patients, who suffered from Breast and/or ovarian cancer and had a strong family history for cancer. To analyze BRCA1/2 punctual variants and copy number variations, we used the Ion Personal Genome Machine (PGM) and Oncomine BRCA1/2 research assay panel. Afterward, we correlated the molecular results with the clinic-pathologic data using IBM SPSS Statistics ver 2. Results From the 64 selected cases, Forty-six had breast cancer, fifteen had ovarian cancer and three had both breast and ovarian cancer. The molecular analysis revealed that 18 patients from the 64 harbored a pathogenic variant (28%). Twelve had six different BRCA1 pathogenic variants and six had six different BRCA2 pathogenic variants. In this study, we report four pathogenic variants that to the best of our knowledge has never been reported in the Moroccan population before. Regarding copy number variation analysis, No CNV was detected in both genes for all the 64 successfully sequenced and analyzed patients in our cohort. Conclusion Work like the present has an important implication on public health and science. It is critical that molecular profiling studies are performed on underserved and understudied population like Morocco.

Penetrance

Surgical oncology

Human genetics

10.1186/s12885-020-07250-0

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Citations (21)

Polymorphisme de l’apolipoprotéine E dans la population du nord du Maroc: fréquence et influence sur les paramètres lipidiques

Pan African Medical Journal (2013)

Fatiha Benyahya Amina Barakat Naima Ghailani Mohcine Bennani Mechita

Introduction: L'objectif de ce travail est de déterminer les fréquences alléliques et génotypiques des sites polymorphes situés dans le gène de l'apolipoprotéine E (apo E) ainsi que leur impact sur les paramètres cliniques et lipidiques dans un échantillon de la population du nord du Maroc cliniquement diagnostiqué ADH.

10.11604/pamj.2013.15.157.2320

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Citations (1)

Etude de la qualité bactériologique de l’eau utilisée dans l’industrie agroalimentaire dans le Nord du Maroc

Pan African Medical Journal (2017)

El Houcine Haijoubi Fatiha Benyahya Abdrezzak Bendahou Faima Zahra Essadqui Mohammed El Behhari

Water is used predominantly in food manufacturing process. Northern morocco agro-food industries use different sources of water, but public water and wells water are the main sources of water used. This water can be the main source of possible food contaminations and alterations. This study aims is to assess the bacteriological quality of water used in the agro-food industries in the Northern region of Morocco, to identify the different types of germs responsible for the pollution of these waters and to establish the main causes of this pollution.Water samples taken from taps or wells were analyzed to detect pollution indicator germs (total coliform (TC), fecal coliform (FC), intestinal enterococci (E), revivable microorganisms (RM), sulphite-reducing anaerobes) and pathogens (Salmonella, Staphylococci, Pseudomonas aeruginosa). The enumeration of the bacteria was performed by filtration technique and incorporation obtained through supercooled solid state.The results showed that public-supply waters were of satisfactory bacteriological quality while 40% of the wells water was non-compliant with water quality standards due to the presence of TC, FC, E and RM pollution indicators. In contrast, pathogens, particularly Salmonellae, were absent in all the wells water analyzed.Well water pollution was generally due to failure to meet hygienic requirements for water pumping. Bacteriological quality of these wells water could be improved by adequate protection.

10.11604/pamj.2017.26.13.10591

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Citations (5)

Effects of different food commodities on larval development and α-amylase activity of Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae)

Journal of Stored Products Research (2008)

Noureddin Bouayad Kacem Rharrabe Naima Ghailani Fouad Sayah

Plodia interpunctella

10.1016/j.jspr.2008.02.012

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Citations (37)

Insecticidal effects of extracts of seven plant species on larval development, α-amylase activity and offspring production of Tribolium castaneum (Herbst) (Insecta: Coleoptera: Tenebrionidae)

Bioresource Technology (2007)

Rachid Jbilou Haitam Amri Noureddin Bouayad Naima Ghailani Abdeslam Ennabili

Pteridium aquilinum

Peganum harmala

10.1016/j.biortech.2007.03.017

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Citations (94)

Chronology of the formation of vesicles and membrane protein aggregates during erythrocyte aging.

PubMed (1995)

Naima Ghailani C. L. Guillemin C. Vigneron

The aim of the present work was to study the changes undergone by proteins in ghost cells, vesicles and membrane protein aggregates during erythrocyte aging. As a model of cell, whole blood collected into CPD was stored for eight weeks at +4 degrees C. SDS-PAGE and immunoblotting with antibodies against spectrin and band 3 showed that vesicles were mainly composed of band 3 and were devoid of spectrin, whereas membrane protein aggregates contained several membrane proteins but in a more advanced state of degradation. A study of spectrin and band 3 in the ghosts, vesicles and protein aggregates revealed increasing fragmentation of both proteins with storage time. Since this degradation was most important in membrane protein aggregates, it was possible to establish the chronological order of appearance of vesicles and aggregates. In view of our observations, we propose that membrane protein aggregates may be regarded as replacement structures resulting from membrane rearrangements occurring after the emission of vesicles.

Band 3

Fragmentation

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Citations (5)