Jin He

Soochow University

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Nancy L. Oleinick

University School

Huilai Zhang

Tianjin Medical University Cancer Institute and Hospital

Lanfang Li

Tianjin Medical University Cancer Institute and Hospital

Xianhuo Wang

Tianjin Medical University Cancer Institute and Hospital

Lihua Qiu

Tianjin Medical University Cancer Institute and Hospital

Shiyong Zhou

Tianjin Medical University Cancer Institute and Hospital

Xiubao Ren

Tianjin Medical University Cancer Institute and Hospital

Bin Meng

Tianjin Medical University Cancer Institute and Hospital

Xianming Liu

Tianjin Medical University Cancer Institute and Hospital

Tingting Zhang

Xijing Hospital

Cooperative Institutions

Tianjin Medical University Cancer Institute and Hospital

Chinese Academy of Medical Sciences & Peking Union Medical College

Case Western Reserve University

National Clinical Research

Johns Hopkins University

Sidney Kimmel Comprehensive Cancer Center

Soochow University

Johns Hopkins Medicine

Peking University

Fudan University

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<title>Signal transduction and metabolic changes during tumor cell apoptosis following phthalocyanine-sensitized photodynamic therapy</title>

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (1993)

Nancy L. Oleinick Munna L. Agarwal Nathan A. Berger M F Cheng Satadel Chatterjee

Mechanisms of cell death have been explored in cells and tumors treated with photodynamic therapy (PDT). Photosensitizers used for these studies were Photofrin, tetrasulfonated and nonsulfonated aluminum phthalocyanine, and a new silicon phthalocyanine [SiPc(OH)OSi(CH₃)₂(CH₂)₃N(CH₃)₂], referred to as PcIV. In mouse lymphoma L5178Y cells, a dose of PDT sensitized by PcIV which causes a 90% loss of cell survival induces apoptosis (programmed cell death) over a several-hour time course, beginning within 10 minutes of irradiation. Apoptosis is a metabolic process initiated by PDT-induced damage to membranes and triggered by the activation of phospholipases A₂ and C and the release of Ca⁺⁺ from intracellular stores. An endogenous endonuclease is activated and cleaves nuclear DNA in the internucleosomal region of chromatin. Subsequent metabolic events now appear to cause the loss of cellular NAD and ATP, the former a result of the activation of a second nuclear enzyme, poly(ADP-ribose) polymerase, by the endonucleolytically generated DNA strand breaks. Loss of ATP follows upon the loss of NAD needed for energy metabolism. Although the induction of apoptosis is efficiently produced by direct PDT damage to L5178Y cells, we now find that apoptosis is also produced by treatment of certain other lymphoid-derived cells and cells of epithelial origin. Under the limited set of conditions tested, there was no evidence for PDT-induced apoptosis in a fibroblast cell line, in mouse fibrosarcoma RIF-1 and L929 cells, in human adenocarcinoma A549 cells, or in human squamous cell carcinoma cells in culture. The evidence suggests that apoptosis, a form of metabolic cell death, is an important mechanism of tumor ablation in PDT-treated tumors, and that the induction of apoptosis may involve the interaction of direct PDT damage to malignant cells with factors produced by PDT action on vascular and other host cells.

Jurkat cells

10.1117/12.146317

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Citations (16)

[Advances in clustered regularly interspaced short palindromic repeats--a review].

PubMed (2011)

Lili Wang Jin He Jieping Wang

The recently discovered Clustered Regularly Interspaced Short Palindromic Repeat (CRISPRs) can protect bacteria and archaea with adaptive and heritable defense systems against the invasion of phage- and plasmid- associated mobile genetic elements. Here, we review the structure, diversity, mechanism of interference and self versus non-self discrimination of CRISPR systems. We also discuss the potential applications of this novel interference system.

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Citations (2)

Promotion of photodynamic therapy-induced apoptosis by stress kinases

Cell Death and Differentiation (1999)

Liang‐yan Xue Jin He Nancy L. Oleinick

10.1038/sj.cdd.4400558

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Citations (79)

The distributed temperature abatement by the phase changing materials for battery in electric tools and its influence on aging

Sustainable Energy Technologies and Assessments (2022)

Ruifei Ma Jin He Xueliang Fan Yaoqiu Qian Yelin Deng

Operating temperature

Heat Generation

10.1016/j.seta.2022.102199

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Citations (4)

Investigation and comparison of the electrochemical impedance spectroscopy and internal resistance indicators for early-stage internal short circuit detection through battery aging

Journal of Energy Storage (2022)

Ruifei Ma Jin He Yelin Deng

Internal resistance

Output impedance

10.1016/j.est.2022.105346

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Citations (20)

[Ex vivo expansion of CD34+ cells from human umbilical cord blood by FL gene transfected bone marrow stromal cells].

PubMed (2003)

Gang Liu Yi Zhang Yuanlin Liu Jin He Xiaoxia Jiang

The object of this study was to explore the effect of FL gene transfected marrow stromal cells on expansion of human cord blood CD34(+) cells ex vivo. A co-culture system was established with FL gene transfected human marrow stromal cells and cord blood CD34(+) cells. The CD34(+) cells were cultured in different culture systems with different combinations of stromal cells and SCF, IL-3 and FL. The numbers of total nucleated cells, CFCs and CD34(+) cells were repeatedly counted in culture systems for 3 weeks. The results showed that the FL transgenic marrow stromal cell feeder layer significantly enhanced the expansion of total nucleated cells, CFC and CD34(+) cells, compared with stromal cell feeder layer and stromal cell-free culture systems. The transgenic stromal cells and cytokines co-culture system manifested the most potent expansion of the cord blood cells with increase of the number of total nucleated cells by (122.5 +/- 4.3)-fold, CFCs by (39.6 +/- 2.7)-fold and CD34(+) cells by (11.8 +/- 0.52)-fold. It is concluded that human umbilical cord blood CD34(+) cells can be extensively expanded ex vivo by using FL gene transfected stromal cells combined with cytokines.

Cord blood

Ex vivo

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Comprehensive Analysis of TP53 Mutation Characteristics and Identification of Patients with Inferior Prognosis and Enhanced Immune Escape in Diffuse Large B Cell Lymphoma

Research Square (Research Square) (2021)

Tingting Zhang Yaxiao Lu Xia Liu Mengmeng Zhao Jin He

Abstract Background: Diffuse large B-cell lymphoma (DLBCL) is a highly heterogeneous lymphatic malignancy. The role of TP53 gene alterations in DLBCL remains unclear. Methods: We performed a comprehensive analysis of the genomic characteristics of TP53 through targeted next-generation sequencing (n=176), RNA-sequencing (n=152), and circulating tumor DNA sequencing (n=38). Results: TP53 was frequently mutated in DLBCL; most TP53 mutations occurred in the DNA-binding domain (DBD). However, TP53 alone is insufficient to effectively differentiate the risk of DLBCL, even when only considering mutations in the DBD region. However, CD58 mutations, which are mutually exclusive from TP53 mutations, in combination with TP53 mutations, could significantly differentiate the prognosis of DLBCL. The survival of patients with either one of the mutually exclusive mutation patterns, namely, TP53MUT&CD58WT or TP53WT&CD58MUT, was inferior to those harboring both wild-type TP53 and CD58. Notably, patients with the TP53WT&CD58MUT mutation pattern had the worst outcome and were characterized by an enhanced immune escape, including features such as the abundant infiltration of inflammatory cells and upregulation of inhibitory immunomodulatory molecules; these patients represent the candidate populations for immune therapy. Conclusions: Our findings indicated that the mutation patterns of TP53 and CD58 accurately stratified patients with DLBCL to permit the optional immunotherapy.

10.21203/rs.3.rs-724635/v1

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Genetic Mutations of Tim-3 Ligand and Exhausted Tim-3+ CD8+ T Cells and Survival in Diffuse Large B Cell Lymphoma

Journal of Immunology Research (2020)

Tingting Zhang Tianyuan Ren Zheng Song Jing Zhao Lei Jiao

Tim-3 is a promising target for antitumor immunotherapy. A number of clinical trials are evaluating the efficacy of anti-Tim-3 therapies as a single agent or combinations in solid tumors and haematologic malignancies. However, there remains a considerable lack of data on Tim-3 signalling, especially the genetic characteristics and immune microenvironment, in diffuse large B cell lymphoma (DLBCL). Herein, we identified three genetic mutations in galectin-9, a major ligand of Tim-3, in six patients with DLBCL (6/188, 3.2%) that were not detected in the COSMIC database. The Oncomine database showed that the mRNA levels of Tim-3 were higher in DLBCL cells than those in normal B cells. Multiplexed immunofluorescence revealed that patients with Tim-3-expressing tumor-infiltrating lymphocytes (Tim-3+ TILs) exhibited poor outcomes than those with Tim-3- TILs (

p = 0.041

). The median survival times of these patients were 65.0 (95% confidence interval (CI): 71.2–88.6) and 79.9 months (95% CI: 54.4–75.6), respectively. Furthermore, we defined a novel subtype of exhausted T cells, named as exhausted Tim-3+ CD8+ T cells, and found that patients with exhausted Tim-3+ CD8+ T cells (median survival, 62.8 months, 95% CI: 50.0–75.6) exhibited shorter survival than those with nonexhausted Tim-3- CD8+ T cells (median survival, 82.5 months, 95% CI: 72.0–92.9;

p = 0.034

). Overall, these findings provide the genetic status of the Tim-3 ligand in DLBCL. Patients with Tim-3+ TILs and exhausted Tim-3+ CD8+ T cells exhibited inferior survival, thus highlighting the possibility of potential therapeutic applications of the inhibition of Tim-3 alone or in combination with other immune checkpoints for treatment of patients with DLBCL.

10.1155/2020/6968595

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Citations (17)

Erratum: Kdm2b promotes induced pluripotent stem cell generation by facilitating gene activation early in reprogramming

Nature Cell Biology (2012)

Gaoyang Liang Jin He Yi Zhang

Reprogramming

Nat

Statement (logic)

10.1038/ncb2518

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Citations (1)