Xinghua Gao

Shanghai University

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Hong‐Duo Chen

China Medical University

Weijia Wen

Hong Kong University of Science and Technology

Cheng Zhou

Second Affiliated Hospital of Zhejiang University

Mary Seabury Stone

Medical University of South Carolina

Prasad Kumarasinghe

University of Western Australia

Cyndi Xu

Medical University of South Carolina

Robert T. Brodell

State Street (United States)

Steffen Emmert

Universitätsmedizin Rostock

Zoe Diana Draelos

Dermatology Consulting Services

Pearl E. Grimes

Vitiligo and Pigmentation Institute of Southern California

Cooperative Institutions

Medical University of South Carolina

293

American Academy of Dermatology

287

UConn Health

287

Chinese Academy of Sciences

China Medical University

Anhui Medical University

First Hospital of China Medical University

University of Chinese Academy of Sciences

Shanghai University

Shanghai Jiao Tong University

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Journal of the American Academy of Dermatology (2023)

Dirk M. Elston Jane M. Grant‐Kels James C. Smith Richard Dobson Jeffrey D. Bernhard

10.1016/s0190-9622(23)00070-1

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Citations (0)

Probing mesenchymal stem cells homing to liver cancer cells on microstructures using microfluidic approach

Jiao Xie Xinghua Gao Weijia Wen

Homing (biology)

Liver Cancer

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Exploratory Evaluation of EGFR-Targeted Anti-Tumor Drugs for Lung Cancer Based on Lung-on-a-Chip

Biosensors (2022)

Jianfeng Tan Xindi Sun Jianhua Zhang Huili Li Jun Kuang

In this study, we used three-dimensional (3D) printing to prepare a template of a microfluidic chip from which a polydimethylsiloxane (PDMS)lung chip was successfully constructed. The upper and lower channels of the chip are separated by a microporous membrane. The upper channel is seeded with lung cancer cells, and the lower channel is seeded with vascular endothelial cells and continuously perfused with cell culture medium. This lung chip can simulate the microenvironment of lung tissue and realize the coculture of two kinds of cells at different levels. We used a two-dimensional (2D) well plate and a 3D lung chip to evaluate the effects of different EGFR-targeting drugs (gefitinib, afatinib, and osimertinib) on tumor cells. The 3D lung chip was superior to the 2D well plate at evaluating the effect of drugs on the NCI-H650, and the results were more consistent with existing clinical data. For primary tumor cells, 3D lung chips have more advantages because they simulate conditions that are more similar to the physiological cell microenvironment. The evaluation of EGFR-targeted drugs on lung chips is of great significance for personalized diagnosis and treatment and pharmacodynamic evaluation.

Afatinib

10.3390/bios12080618

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Citations (21)

[Protective effect of mouse 2.5s nerve growth factor on PC12 cells from injury induced by 2, 5-hexanedione].

PubMed (2006)

Ling-Cong Sun Xia Laju Xiang-ping Meng Li Liu Xinghua Gao

To explore whether the nerve growth factor has protective effects on PC12 cells from injury induced by 2, 5-hexanedione.With PC12 cells as the model of neurons, different concentrations of NGF were added into the culture of PC12 cells. Then cell viability was tested with MTT. The DNA fragment was observed with agarose gel electrophoresis. The apoptosis ratio was tested with flow cytometry (FACS). The p53 protein was detected with western blot. The differences among the groups were compared.Cell viabilities were increased with the increase of the concentrations of NGF (P < 0.05). The DNA fragment, the apoptosis ratio and the expression of p53 were all decreased with the increase of the concentrations of NGF (P < 0.05).The NGF might have direct nutritional effects on PC12 cells, and protect them from injury induced by 2, 5 HD. Moreover, it might also have anti-apoptosis effect to some extent.

Agarose gel electrophoresis

MTT assay

Agarose

Viability assay

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Citations (2)

Hollow Microfiber Assembly‐Based Endocrine Pancreas‐on‐a‐Chip for Sugar Substitute Evaluation (Adv. Healthcare Mater. 21/2024)

Advanced Healthcare Materials (2024)

Lingling Tian Jingyan Shi Wei Li Yuan Zhang Xinghua Gao

Microfiber

10.1002/adhm.202470135

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Spatiotemporal Single-Cell Rna Sequencing Reveals that Cd4+T Subpopulation with High Metabolic Flux Plays Pivotal Roles Under in Hyperthermia Treatment Warts

Xueli Niu Zhe Long Mengfei Wang Bin Bai Xinyun Fan

The features of local hyperthermia achieved clearance of multiple warts by targeting a single lesion, strongly suggest that it may activate T cells responses. In this study, we profiled T cells within verruca after hyperthermia treatment using single-cell RNA sequencing and spatial enhanced resolution omics sequencing. A new subpopulation of T cells was found that exhibited high metabolic fluxes and proliferation relative to other cells, which we defined as CD4+T proliferation (CD4_Tp) cells. The CD4_Tp cells showed a high communication with CD8+T cells, they could lead to increased activation of CD8+T cells by secreting of PLCB2. The functional analysis showed that CD8+T cells increased the expression of functional molecules including GZMB, TNFRSF4 and TNFSF10 after in response to hyperthermia, and these findings were validated by multiplex immunohistochemistry staining. Our study revealed that the CD4_Tp cells may be a potential therapeutic strategy for clearing viral warts.

10.2139/ssrn.4636648

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Editorial board

Journal of the American Academy of Dermatology (2023)

Dirk M. Elston Jane M. Grant‐Kels James C. Smith Richard Dobson Jeffrey D. Bernhard

10.1016/s0190-9622(23)03049-9

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Micro- and nano-fibers for organ-on-a-chip: Construction, applications, and prospects

Materials Today Bio (2024)

Xiaoling Yang Jingyan Shi Bori Shi Jianing Li Chang Xue

Organ-on-a-Chip

3D cell culture

Organ culture

10.1016/j.mtbio.2024.101322

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The application of nanoemulsion in dermatology: an overview

Journal of drug targeting (2013)

Yan Wu Yuan-Hong Li Xinghua Gao Hong‐Duo Chen

Nanotechnology has been introduced into dermatology for years. Nanoemulsions (NEs) are promising drug delivery systems with practical applications for pharmaceutical, cosmetic and chemical industry applications. Herein, we provide an overview of the application of NEs in dermatology during the latest 5 years. We reviewed the antioxidants in NEs form, non-steroidal anti-inflammatory drug loaded by NE, NEs in photodynamic therapy, NEs in decontamination of radionuclides, antimicrobial NEs, NEs carrying lipids, NEs containing Octyl Methoxycinnamate, et al. NEs demonstrate good stability, stable physical and chemical properties. NEs are able to enhance the functionality and efficacy of active chemicals and natural ingredients. NEs exhibit great application potential in the field of dermatology.

Cosmetic Industry

10.3109/1061186x.2013.765442

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Citations (49)

Thalidomide improves clinical symptoms of primary cutaneous amyloidosis: report of familiar and sporadic cases

Dermatologic Therapy (2013)

Qian An Li Zhang Song Guo Zheng Junping Lin Yuxiao Hong

Primary cutaneous amyloidosis (PCA), either familial or sporadic, poses a therapeutic challenge. We conducted an open trial using thalidomide to treat three cases of familial and three cases of sporadic PCA, at initial dose of 100 mg/day. Dosage adjustment was made according to improvement of symptoms or patient's own choice. All except one sporadic case experienced moderate to significant relief on the symptoms of itching, over an observational period of 8 weeks by visual analog score (from 8.08 ± 0.88 to 1.60 ± 0.68, on average) as well as clinical amelioration of symptoms. Side effects included fatigue, drowsiness, numbness, and facial and leg edema in some of the patients. From the present observation, it seems that thalidomide is a promising drug to treat PCA.

Itching

10.1111/j.1529-8019.2013.01548.x

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Citations (1)