The relationship between the induction of tumor necrosis factor (TNF) (as an indicator of inflammatory reaction) in tumor tissues and its antitumor effect was investigated in tumor-bearing mice by using nine biologic response modifiers (BRMs) and by exogenous/endogenous TNF therapy following a previously reported protocol. Close correlation between the induction of TNF-rich inflammation in tumor tissues and the antitumor effect of BRM were observed. The results of this study suggest that the conditions necessary for exerting antitumor effects of biologic response modifiers may be the induction of TNF (50 to 200 U/g) at the tumor lesions at an early stage after BRM administration and maintenance of the detectable amount of TNF (approximately 10 U/g) for more than 6 hours. Tumor necrosis factor should also be induced in the liver and spleen so that its activity can be maintained in the tumor lesions.
Anionic and cationic resins-adsorbed fractions of 44 lichens, hot water extracts of 9 lichens, and 20 lichen metabolites and their degradation products were assayed for their antitumor activity by the use of ascitic or solid-type Ehrlich carcinoma. Among them, the adsorbed fraction of Ramalina almquistii VAIN., d-protolichesterinic acid (1) and nephrosterinic acid (2) were effective against the solid-type Ehrlich carcinoma.
Journal Article Analysis of RNA Synthesized in Isolated Nuclei of Ehrlich Ascites Tumor Cells Get access Yoshinobu NAKANISHI, Yoshinobu NAKANISHI Faculty of Pharmaceutical Sciences, University of TokyoHongo, Bunkyo-ku, Tokyo 113 Search for other works by this author on: Oxford Academic PubMed Google Scholar Den'ichi MIZUNO, Den'ichi MIZUNO Faculty of Pharmaceutical Sciences, University of TokyoHongo, Bunkyo-ku, Tokyo 113 Search for other works by this author on: Oxford Academic PubMed Google Scholar Shunji NATORI Shunji NATORI Faculty of Pharmaceutical Sciences, University of TokyoHongo, Bunkyo-ku, Tokyo 113 Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 83, Issue 4, April 1978, Pages 989–994, https://doi.org/10.1093/oxfordjournals.jbchem.a132027 Published: 01 April 1978 Article history Received: 20 October 1977 Published: 01 April 1978
Abstract Pyrophosphatase activity of rat liver phosphodiesterase, which forms nucleoside 5'-monophosphates, was studied. Evidence is presented that NADH, adenosine 5'-diphosphate ribose, and a polymer of phosphoribosyl-AMP (poly (ADPR)) were hydrolyzed by this enzyme. The mode of action of the enzyme on poly ADPR was studied. 1. The enzyme hydrolyzed poly ADPR to produce the same product as was formed by phosphodiesterase from snake venom. 2. Kinetic studies suggest that both the phosphodiester compounds, p-nitrophenyl urinine 5'-monophosphate and poly ADPR, were hydrolyzed by the same site or by overlapping active sites. 3. Poly ADPR was not hydrolyzed randomly, but rather in an exonucleolytic fashion. Little DNA or RNA was hydrolyzed by an amount of enzyme sufficient to decompose half the initial amount of the substrate, poly ADPR.
4-Amino-1-(o-, m-, or p-nitrophenyl)-1H-pyrazolo [3, 4-d] pyrimidine (Io, Im, or Ip), and 41 kinds of their derivatives were synthesized and the compounds were screened for antitumor activity against Ehrlich carcinoma. Antitumor activity was found in 4-elaidamido-1-(o-nitrophenyl)-1H-pyrazolo [3, 4-d] pyrimidine (VIIIo) and 4-oleamido-1-(p-nitrophenyl) compound (IXp).
Ten derivatives of isothiourea including 8 new compounds were synthesized and screened for the anti-tumor activity. Twenty nine derivatives of azine, 27 thiourea derivatives, 6 thiazole derivatives, 20 triazoline derivatives and 7 guanidine derivatives were also synthesized and screened for the anti-tumor activity. The anti-tumor activity was assayed by the use of ascitic or solid type of Ehrlich carcinoma. Among them, N-phenyl-S-benzylthioisothiourea·HCl, S-furfurylthioisothiourea·HCl, N-phenyl-S-furfurylthioisothiourea·HCl, 4, 4'-diacetylaminobenzalazine and 2, 3-dihydro-4-methyl-2-(2'-pyridylmethylenehydrazono)thiazole·2HCl were effective against the solid type of Ehrlich carcinoma.
