Luiz Gustavo Rodrigues Oliveira

Universidade Federal do Oeste da Bahia

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Christian Collins Kuehn

Universidade Federal de Rondônia

José Clóvis do Prado Júnior

Universidade de São Paulo

Carla Domingues Santos

Universidade de São Paulo

Mariza Abreu Miranda

University of Florida

Míriam Paula Alonso Toldo

Universidade de São Paulo

Cássia Mariana Bronzon da Costa

Universidade de São Paulo

Renata Fabiane Jorge Tiossi

Universidade de São Paulo

Vânia Brazão

Universidade de São Paulo

James D. McChesney

University of Mississippi

Ana Amélia Carraro Abrahão

Universidade de São Paulo

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Universidade de São Paulo

Universidade Federal do Oeste da Bahia

Universidade de Ribeirão Preto

Universidade Federal do Paraná

Hospital Universitário da Universidade de São Paulo

Universidade Federal da Bahia

Instituto de Ciências Farmacêuticas

Universidade de Franca

Universidade Estadual Paulista (Unesp)

Universidade Estadual de Campinas (UNICAMP)

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Efeitos da administração de selênio na prenhez de ratas wistar durante a fase aguda da infecção chagásica

Cássia Mariana Bronzon da Costa Carla Domingues dos Santos Sponchiado Vânia Brazão Christian Collins Kuehn Luiz Gustavo Rodrigues Oliveira

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In vitro Leishmanicidal and Cytotoxic Activities of the Glycoalkaloids from Solanum lycocarpum (Solanaceae) Fruits

Chemistry & Biodiversity (2013)

Mariza Abreu Miranda Renata Fabiane Jorge Tiossi Mariana Rosa da Silva Kelly Cristina Rodrigues Christian Collins Kuehn

Abstract Leishmaniasis is an infection caused by a protozoan parasite of the genus Leishmania and is the second most prevalent parasitic protozoal disease after malaria in the world. We report the in vitro leishmanicidal activity on promastigote forms of Leishmania amazonensis and cytotoxicity, using LLCMK 2 cells, of the glycoalkaloids from the fruits of Solanum lycocarpum , determined by colorimetric methods. The alkaloidic extract was obtained by acid‐base extraction; solamargine and solasonine were isolated by silica‐gel chromatography, followed by reversed‐phase HPLC final purification. The alkaloidic extract, solamargine, solasonine, as well as the equimolar mixture of the glycoalkaloids solamargine and solasonine displayed leishmanicidal activity against promastigote forms of L. amazonensis , whereas the aglycone solasodine was inactive. After 24 and 72 h of incubation, most of the samples showed lower cytotoxicities ( IC 50 6.5 to 124 μ M ) as compared to leishmanicidal activity ( IC 50 1.1 to 23.6 μ M ). The equimolar mixture solamargine/solasonine was the most active with an IC 50 value of 1.1 μ M , after 72 h. Likewise, solamargine was the most active after 24 h with an IC 50 value of 14.4 μ M , both in comparison with the positive control amphotericin B.

Solasodine

10.1002/cbdv.201200063

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Citations (38)

Effects of dehydroepiandrosterone-sulfate (DHEA-S) and benznidazole treatments during acute infection of two different Trypanosoma cruzi strains

Immunobiology (2010)

Carla Domingues Santos Roger M. Loria Luiz Gustavo Rodrigues Oliveira Christian Collins Kuehn Míriam Paula Alonso Toldo

Benznidazole

10.1016/j.imbio.2009.11.002

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Citations (22)

Potenciais ações terapêuticas da associação melatonina-meloxicam sobre a cardiomiopatia chagásica crônica em ratos wistar infectados pela cepa Y de Trypanosoma cruzi

Luiz Gustavo Rodrigues Oliveira

O sistema imune do hospedeiro acometido pela doença de Chagas tem sido alvo de importantes pesquisas que podem favorecer avanços no entendimento dos mecanismos patogênicos relacionados à doença.No presente estudo, investigamos possíveis ações benéficas provenientes de efeitos anti-inflamatórios e antioxidantes da associação terapêutica (melatonina/meloxicam) sobre a cardiomiopatia chagásica crônica.Foram analisadas citocinas e outros mediadores envolvidos no processo inflamatório característico da infecção.Além disso, níveis plasmáticos de Creatina Fosfoquinase MB (CK-MB), número de focos inflamatórios no tecido cardíaco e análise do peso relativo do coração foram avaliados como fatores preditivos da redução dos danos no miocárdio após os diferentes tratamentos.Como modelo experimental, foram utilizados ratos wistar infectados pela cepa Y de T. cruzi e tratados ou não com melatonina, meloxicam, ou associação de ambos.As células inflamatórias e o parasitismo tecidual cardíaco foram avaliados por meio de técnica histológica.A técnica ELISA foi utilizada para detecção e quantificação de citocinas pró-inflamatórias e modulatórias no miocárdio e nos soros dos animais experimentais.Também foram investigados os níveis de nitrito produzidos por macrófagos e por células cardíacas dos grupos experimentais pela reação de Griess.Os parâmetros foram investigados

Meloxicam

10.11606/t.60.2013.tde-18122013-094656

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The New Coronavirus (SARS-CoV-2): A Comprehensive Review on Immunity and the Application of Bioinformatics and Molecular Modeling to the Discovery of Potential Anti-SARS-CoV-2 Agents

Molecules (2020)

Gustavo Roberto Villas Boas Vanessa Cristina Rescia Marina Meirelles Paes Stefânia Neiva Lavorato Manoel F. de Magalhães-Filho

On March 11, 2020, the World Health Organization (WHO) officially declared the outbreak caused by the new coronavirus (SARS-CoV-2) a pandemic. The rapid spread of the disease surprised the scientific and medical community. Based on the latest reports, news, and scientific articles published, there is no doubt that the coronavirus has overloaded health systems globally. Practical actions against the recent emergence and rapid expansion of the SARS-CoV-2 require the development and use of tools for discovering new molecular anti-SARS-CoV-2 targets. Thus, this review presents bioinformatics and molecular modeling strategies that aim to assist in the discovery of potential anti-SARS-CoV-2 agents. Besides, we reviewed the relationship between SARS-CoV-2 and innate immunity, since understanding the structures involved in this infection can contribute to the development of new therapeutic targets. Bioinformatics is a technology that assists researchers in coping with diseases by investigating genetic sequencing and seeking structural models of potential molecular targets present in SARS-CoV2. The details provided in this review provide future points of consideration in the field of virology and medical sciences that will contribute to clarifying potential therapeutic targets for anti-SARS-CoV-2 and for understanding the molecular mechanisms responsible for the pathogenesis and virulence of SARS-CoV-2.

Pandemic

Coronavirus

Molecular virology

10.3390/molecules25184086

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Citations (18)

Prior and concomitant dehydroepiandrosterone treatment affects immunologic response of cultured macrophages infected with Trypanosoma cruzi in vitro?

Veterinary Parasitology (2010)

Christian Collins Kuehn Luiz Gustavo Rodrigues Oliveira Carla Domingues Santos Mariana B. Augusto Míriam Paula Alonso Toldo

10.1016/j.vetpar.2010.12.009

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Protective actions of melatonin against heart damage during chronic Chagas disease

Acta Tropica (2013)

Luiz Gustavo Rodrigues Oliveira Christian Collins Kuehn Carla Domingues Santos Mariza Abreu Miranda Cássia Mariana Bronzon da Costa

Chagas Disease

Creatine kinase

10.1016/j.actatropica.2013.09.014

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Citations (26)

Distinctive histopathology and modulation of cytokine production during oral and intraperitonealTrypanosoma cruziY strain infection

Parasitology (2014)

Christian Collins Kuehn Luiz Gustavo Rodrigues Oliveira Mariza Abreu Miranda José Clóvis do Prado Júnior

SUMMARY Acute Chagas disease outbreaks are related to the consumption of food or drink contaminated by triatomine feces, thus making oral infection an important route of transmission. Both vector-borne and oral infections trigger important cardiac manifestations in the host that are related to a dysregulated immune response. The aims of this work were to evaluate possible alterations of lymphocyte CD 4+ /CD 8+ sub-populations, Th1 and Th2 cytokines, nitrite concentrations and cardiac histopathology. One group of male Wistar rats was intraperitoneally infected (I.P.) with 1×10 5 metacyclic trypomastigotes of the T. cruzi Y strain, and another group of Wistar rats was orally infected (O.I.) with 8×10 5 metacyclic trypomastigotes of the same strain. The intraperitoneal infection triggered statistically enhanced parasite and peritoneal macrophage numbers, increased concentrations of NO and IL-12 and elevated cardiac inflammatory foci when compared with the oral infection. However, proliferation of CD 4+ and CD 8+ T cells were not statistically different for oral and intraperitoneal routes.

Histopathology

Chagas Disease

Intraperitoneal injection

Oral infection

10.1017/s0031182013002059

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Clinical, epidemiological and transmission cycle aspects of leishmaniasis urbanization in Barreiras, Bahia, Brazil

Spatial and Spatio-temporal Epidemiology (2020)

Alexandre Faria Alvares Lacerda Denise Santana Oliveria Juliane Vilela Fereira Salomão Luiz Gustavo Rodrigues Oliveira Adriano Figueiredo Monte-Alegre

10.1016/j.sste.2020.100395

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