Guibo Sun

Chinese Academy of Medical Sciences & Peking Union Medical College

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Xiaobo Sun

Chinese Academy of Medical Sciences & Peking Union Medical College

Yun Luo

Chinese Academy of Medical Sciences & Peking Union Medical College

Xiaobo Sun

State Administration of Traditional Chinese Medicine of the People's Republic of China

Min Wang

Chinese Academy of Medical Sciences & Peking Union Medical College

Jingxue Ye

Chinese Academy of Medical Sciences & Peking Union Medical College

Xiangbao Meng

Shenzhen Second People's Hospital

Shan Lu

Chinese Academy of Medical Sciences & Peking Union Medical College

Xuelian Zhang

China-Japan Friendship Hospital

Ruiying Wang

Chinese Academy of Medical Sciences & Peking Union Medical College

Chenyang Zhang

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

348

Peking Union Medical College Hospital

Chinese Academy of Sciences

State Administration of Traditional Chinese Medicine of the People's Republic of China

Peking University

Ministry of Education of the People's Republic of China

Capital Medical University

Sun Yat-sen University

Huazhong University of Science and Technology

Sichuan University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Corrigendum: Notoginsenoside R1 protects against diabetic cardiomyopathy through activating estrogen receptor α and its downstream signaling

Frontiers in Pharmacology (2025)

Bin Zhang Jingyi Zhang Chenyang Zhang Xuelian Zhang Jingxue Ye

Corrigendum on: Zhang B, Zhang J, Zhang C, Zhang X, Ye J, Kuang S, Sun G and Sun X (2018) Notoginsenoside R1 Protects Against Diabetic Cardiomyopathy Through Activating Estrogen Receptor α and Its Downstream Signaling. Front. Pharmacol. 9:1227Pharmacol. 9: . doi: 10.3389/fphar.2018.01227 .01227 In the published article, there was an error in Figures 2F, Figure 3A, E and Figures 8C as published. Specifically, Western blot pictures of HO-1and NQO-1 in Figure 2F were misplaced.After checking the raw data, the misplaced Western blot pictures of HO-1and NQO-1 were corrected. The corrected Figures 2 and its caption appears below.In the published article, there was an error in Figures 3A,E. The TUNEL staining picture in AGEs group was misplaced in Figures 3A. Additionally, the Western blot pictures of Bcl-2 and cleaved casp-3 were misplaced in Figure 3E. After checking the raw data, the misplaced bands of Bcl-2 and cleaved casp-3 were corrected. The misplaced Lamin B1 and β-actin bands in Figure 8C were also corrected. The corrected Figures 3 and8 and its caption appears below. and HO-1) showed that NGR1 promoted the translocation of Nrf2 to the nucleus. This was associated with the up-regulation of these anti-oxidant enzymes. The quantitative data are presented as the mean ± SD (n = 3). ## P < 0.01 vs. the Control group, * P < 0.05 or ** P < 0.01 vs. the AGEs group.The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Diabetic Cardiomyopathy

GPER

10.3389/fphar.2024.1488083

Cite

Citations (0)

Advanced Activity-Based Protein Profiling Application Strategies for Drug Development

Frontiers in Pharmacology (2018)

Shan Wang Yu Tian Min Wang Min Wang Guibo Sun

Drug targets and modes of action remain two of the biggest challenges in drug development. To address these problems, chemical proteomic approaches have been introduced to profile targets in complex proteomes. Activity-based protein profiling (ABPP) is one of a growing number chemical proteomic approaches that uses small-molecule chemical probes to understand the interaction mechanisms between compounds and targets. ABPP can be used to identify the protein targets of small molecules and even the active sites of target proteins. This review focuses on the overall workflow of the ABPP technology and on additional advanced strategies for target identification and/or drug discovery. Herein, we mainly describe the design strategies for small-molecule probes and discuss the ways in which these probes can be used to identify targets and even validate the interactions of small molecules with targets. In addition, we discuss some basic strategies that have been developed to date, such as click chemistry-ABPP, competitive strategies and, recently, more advanced strategies, including isoTOP-ABPP, fluoPol-ABPP, and qNIRF-ABPP. The isoTOP-ABPP strategy has been coupled with quantitative proteomics to identify the active sites of proteins and explore whole proteomes with specific amino acid profiling. FluoPol-ABPP combined with HTS can be used to discover new compounds for some substrate-free enzymes. The qNIRF-ABPP strategy has a number of applications for in vivo imaging. In this review, we will further discuss the applications of these advanced strategies.

Proteome

Profiling (computer programming)

10.3389/fphar.2018.00353

Cite

Citations (82)

Cardioprotective effects of Notoginsenoside R1 against ischemia/reperfusion injuries by regulating oxidative stress- and endoplasmic reticulum stress- related signaling pathways

Scientific Reports (2016)

Yingli Yu Guibo Sun Yun Luo Min Wang Rongchang Chen

Abstract Background : Recent reports suggested the involvement of oxidative stress- and endoplasmic reticulum stress (ERS)-associated pathways in the progression of ischemia/reperfusion (I/R) injury. Notoginsenoside R1 (NGR1) is a novel saponin isolated from P. notoginseng , which has a history of prevention and treatment of cardiovascular diseases. Objective : We aimed to examine the cardioprotective effects of NGR1 on I/R-induced heart dysfunction ex vivo and in vitro. Methods : H9c2 cadiomyocytes were incubated with NGR1 for 24 h and exposed to hypoxia/reoxygenation. Isolated rat hearts were perfused by NGR1 for 15 min and then subjected to global ischemia/reperfusion. Hemodynamic parameters were monitored as left ventricular systolic pressure (LVSP), heart rate, and maximal rate of increase and decrease of left ventricular pressure (±dP/dt max/min). Results : NGR1 pretreatment prevents cell apoptosis and delays the onset of ERS by decreasing the protein expression levels of ERS-responsive proteins GRP78, P-PERK, ATF6, IRE, and inhibiting the expression of pro-apoptosis proteins CHOP, Caspase-12, and P-JNK. Besides, NGR1 scavenges free radical, and increases the activity of antioxidase. NGR1 inhibits Tunicamycin-induced cell death and cardic dysfunction. Conclusion : We elucidated the significant cardioprotective effects of NGR1 against I/R injuries, and demonstrated the involvement of oxidative stress and ERS in the protective effects of NGR1.

Panax notoginseng

Tunicamycin

ATF6

10.1038/srep21730

Cite

Citations (133)

[Research progress on pharmacology of butylphthalide and its derivatives].

PubMed (2022)

Haiyan Xiao Shuxian Yang Wen Han Li Cao Guibo Sun

The butylphthalide(NBP), a colorless or light yellow viscous oily component isolated from celery seeds, has the effects of anti-inflammation, anti-oxidative stress, protecting blood-brain barrier, improving cerebral microcirculation, and promoting angiogenesis. It can protect the neurological function of patients with ischemic stroke through a variety of mechanisms, improve the symptoms of patients, and contribute to the long-term recovery of them. Therefore, independently developed in China, NBP was approved by State Food and Drug Administration for the clinical treatment of stroke patients in 2002. At the same time, owing to the complex multi-target pharmacological mechanism, NBP has been frequently used in clinical practice. As frequently verified, it has obvious effects in the treatment of other neurological diseases such as Alzheimer's disease, vascular dementia, Parkinson's disease, autoimmune diseases, depression, traumatic central nervous system injury. Moreover, it demonstrates significant pharmacological effects on non-neurological diseases such as diabetes mellitus and myocardial infarction. Therefore, this study summarizes the research progress on roles of NBP in nervous system diseases and non-nervous system diseases, and the pharmacological characteristics and mechanisms of NBP, which is expected to lay a basis for research on related targets.

Stroke

Depression

10.19540/j.cnki.cjcmm.20220117.701

Cite

Citations (2)

Effects of the Tangningtongluo formula as an alternative strategy for diabetics via upregulation of insulin receptor substrate-1

Molecular Medicine Reports (2017)

Long Cheng Song Jun-mei Li Geng Yue Liu Yuming Wang

Tangningtongluo (TNTL), a traditional Chinese medicine, has been widely used in clinics for decades in southwest China. Its pharmacological properties and underlying molecular mechanisms remain unclear. The main goal of ethnopharmacology is to identify novel bioactive compounds derived from plants for use in indigenous medical practice. This knowledge can be used to develop novel pharmaceuticals. In the present study, hyperglycemic C57BL/KsJ‑db/db (db/db) mice were used to test the effect of TNTL on microvasculature of the retina and hypoglycemia. Metformin (Met) was selected as a positive control. 26‑week‑old mice were randomly assigned to receive either the anti‑diabetic agent Met [140 mg/kg body weight (BW)], 1.8, 0.9 or 0.45 g/kg BW TNTL, or a placebo. The fasting blood glucose, serum insulin and glycated hemoglobin levels were measured. Histopathologic examination of the pancreas was performed to confirm the hypoglycemic effect. Fluorescein angiography was applied to detect diabetes‑induced retinal angioma in the db/db mice. TNTL intake significantly decreased the fasting blood glucose level in a dose‑dependent manner. Additionally, TNTL intervention resulted in a significant decrease in the insulin resistance index. Notably, TNTL treatment markedly reduced the speed of retinal degeneration and mildly reversed microvascular caliber degeneration. Western blot analysis indicated that upregulation of phosphorylated insulin receptor substrate‑1 (p‑IRS‑1) by the administration of TNTL may be strongly involved in the improvement of insulin resistance. In conclusion, TNTL exerted a strong hypoglycemic effect and reversed retinal degeneration via upregulation of ISR‑1. The present findings provide important scientific evidence supporting TNTL as an effective alternative approach for the management of Type 2 diabetes mellitus.

Glycated hemoglobin

IRS1

10.3892/mmr.2017.6679

Cite

Citations (8)

Ginsenoside F1 Ameliorates Endothelial Cell Inflammatory Injury and Prevents Atherosclerosis in Mice through A20-Mediated Suppression of NF-kB Signaling

Frontiers in Pharmacology (2017)

Meng Qin Yun Luo Shan Lu Jing Sun Ke Yang

Atherosclerosis (AS) is a chronic inflammatory disease and endothelial cell injury is the initial event. In this study, we investigated the protective effects of ginsenoside F1 (GF1) on AS and the potential molecular mechanisms of ox-LDL induced endothelial injury. ApoE-/- mice were fed a high fat diet and orally treated with GF1 (50 mg/kg/day) for 8 weeks. Atherosclerotic plaque and LOX-1, TLR4, NF-κB expression levels in the aortic root and inflammatory factor MPO in whole body were measured. The treatment with GF1 induced a remarkable reduction in the atherosclerotic lesion area, LOX-1, TLR4 expression and decreased the MPO distribution. Meanwhile, in vitro study, we confirmed that GF1 treatment greatly increased ox-LDL-injured endothelial cell viability, ameliorated LOX-1, TLR4 expression levels and reduced monocytes adhesion. Protein microarray demonstrated that GF1 significantly inhibited G-CSF, ICAM-1, MIP-1δ, IL-1α, IL-15, IL-16 levels. Mechanistically, the GF1 treatment suppressed the NF-κB nuclear translocation. Furthermore, our data indicated that GF1 significantly increased A20 expression level and A20 siRNA markedly abolished the attenuation of GF1 on NF-κB nuclear translocation and inflammatory factors expression. Our results suggest that the GF1 may be a potential drug for anti-atherosclerosis.

Phorbol

10.3389/fphar.2017.00953

Cite

Citations (48)

[Protective effects and underlying mechanisms of Panax notoginseng saponins against SH-SY5Y cell apoptosis induced by 6-hydroxydopamine].

PubMed (2016)

Meng-xia Wang Jingyu Zhao Dongmei Sun Xiangbao Meng Guibo Sun

The aim of this study is to investigate the protective effects of Panax notoginseng saponins (PNS) against 6-hydroxydopamine(6-OHDA)-induced apoptosis in SH-SY5Y cells and the possible underlying mechanisms. Cell viability was examined by MTT assay. The levels of lactate dehydrogenase(LDH), reactive oxygen species (ROS), malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GSH-Px) were measured using the respective assay kits. Apoptosis was measured by TUNEL kit, JC-1 and ROS was measured by staining with fluorescent dyes. The activation of caspase-3 was measured with the caspase-3 assay kit. The expression of nuclear protein Nrf2 and HO-1 were determined by Western blot. PNS had significant protective effects against 6-OHDA-induced apoptosis in SH-SY5Y cells in a time- and dose-dependent manner. PNS could attenuate 6-OHDA-induced suppression of SOD, GAT, GSH-Px (P < 0.01). PNS reduced the level of LDH, decreased the levels of ROS, MDA and increased cell viability and the mitochondrial membrane potential (P < 0.01). PNS also inhibited DNA fragmentation, mitochondrial response and the activation of caspase-3 (P < 0.01). Moreover, PNS pretreatment increased the expression of the nuclear Nrf2 and up-regulate HO-1. The protective effects of PNS could be inhibited by HO-1 inhibitor SnPP. In conclusion, PNS has significant protective effects against 6-OHDA- induced apoptosis in SH-SY5Y cells. The possible mechanisms of PNS are due to PNS-mediated activation of Nrf2, up-regulation of HO-1 and inhibition of oxidative stress.

Panax notoginseng

Viability assay

Hydroxydopamine

MTT assay

SH-SY5Y

Source

Cite

Citations (3)

Aconitine-induced Ca2+ overload causes arrhythmia and triggers apoptosis through p38 MAPK signaling pathway in rats

Toxicology and Applied Pharmacology (2014)

Guibo Sun Hong Sun Xiangbao Meng Jin Hu Qiang Zhang

Aconitine