Background: To identify neoplastic and reactive neoformation bone by quantitative CT radiomics analysis, and to further validate the performance of the model by the diagnosis of osteosarcoma (OS) and chondrosarcoma (CS).Methods: A total of 478 bone tumor patients were retrospectively enrolled from 5 hospitals. Crucial radiomics features were selected and employed to construct radiomics signature by Least absolute shrinkage and selection operator (LASSO) regression. Then radiomics signature and clinical characteristics were combined to construct the model identifying neoplastic and reactive neoformation bone (ModelRN) in the training set (n = 300), and then validated in the validation set (n = 178). Furthermore, a new model (ModelOC) incorporating the clinical characteristics and the comprehensive scores produced by ModelRN was constructed to differentiate OS and CS (training set: n = 103; validation set: n = 41). The performance of the model was evaluated with respect to discrimination, calibration, and clinical usefulness. Findings: ModelRN incorporating radiomics signature, tumor position and course of disease achieved promising results with high identification accuracy (AUC: 0.9319 in the training set and 0.9350 in the validation set). ModelOC was built incorporating alkaline phosphatase with the comprehensive score produced by ModelRN. ModelOC also showed satisfying performance in the training (AUC: 0.8752) and validation (AUC: 0.9032) sets. Decision curve analysis showed the clinical usefulness of the model.Interpretation: This study suggested that CT-based radiomics can help radiologists to identify neoplastic and reactive neoformation bone, thereby more effectively distinguishing osteosarcoma from chondrosarcoma than current clinical images diagnosis.Funding Statement: This paper is supported by the National Natural Science Foundation of China (Grant Nos. 81871510, 81871511), Ministry of Science and Technology of China under Grant (No.2017YFA0205200, 2017YFA0700401, 2016YFA0100902, 2016YFC0103803, 2016YFA0201401, 2016YFC0103702).Declaration of Interests: The authors declare no potential conflicts of interest.Ethics Approval Statement: Ethical approval was obtained from the institutional review board (IRB) of the Third Affiliated Hospital of Southern Medical University, and the need for informed consent from the patients was waived.
Background lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and is associated with the development of breast cancer. However, the role of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells ,remain largely unknown. Methods We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods.Then,We evaluated the effects of HOTAIR and miRNA-1 expression on the biological behavior of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, Clonogenic assays, Transwell assay and Flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally,The target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by Luciferace reports. Results The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues（P＜0.05）. Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer（P＜0.0001）. We also verified the existence of paired sequences between miR-1 and HOTAIR, GOLPH3 and miR-1 by Luciferace reports (P＜0.001）. Conclusions The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited cell proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells.
Tumor promote disease progression by reprogramming their metabolism and that of distal organs, so it is of great clinical significance to study the changes in glucose metabolism at different tumor stages and their effect on glucose metabolism in other organs.A retrospective single-centre study was conducted on 253 NSCLC (non-small cell lung cancer) patients with negative lymph nodes and no distant metastasis. According to the AJCC criteria, the patients were divided into different groups based on tumor size: stage IA, less than 3 cm (group 1, n = 121); stage IB, greater than 3-4 cm (group 2, n = 64); stage IIA, greater than 4-5 cm (group 3, n = 36); and stage IIB, greater than 5-7 cm (group 4, n = 32). All of the patients underwent baseline 18F-FDG PET/CT scans, and the primary lesion SUVmax (maximum standardized uptake value), liver SUVmean (mean standardized uptake value), spleen SUVmean, TLR (Tumor-to-liver SUV ratio) and TSR (Tumor-to-spleen SUV ratio) were included in the study, combined with clinical examination indicators to evaluate DFS (disease free survival).In NSCLC patients, with the increase in the maximum diameter of the tumor, the SUVmax of the primary lesion gradually increased, and the SUVmean of the liver gradually decreased. The primary lesion SUVmax, liver SUVmean, TLR and TSR were related to disease recurrence or death. The best predictive parameters were different when the tumor size differed. SUVmax had the highest efficiency when the tumor size was less than 4 cm (AUC:0.707 (95% CI, 0.430-0.984) tumor size < 3 cm), (AUC:0.726 (95% CI, 0.539-0.912) tumor size 3-4 cm), liver SUVmean had the highest efficiency when the tumor size was 4-5 cm (AUC:0.712 (95% CI, 0.535-0.889)), and TLR had the highest efficiency when the tumor size was 5-7 cm [AUC:0.925 (95%CI, 0.820-1.000)].In patients with early NSCLC, glucose metabolism reprogramming occurs in the primary lesion and liver. With the increase in tumor size, different metabolic parameters should be selected to evaluate the prognosis of patients.
At present, the primary treatment of esophageal cancer is surgery-based comprehensive treatment, including adjuvant therapy such as chemotherapy and/or radiotherapy. However, the role of adjuvant therapy for esophageal squamous cell carcinoma (ESCC) with pathologically node-negative (pN0) disease is controversial. This study aimed to evaluate the impact of postoperative adjuvant therapy on survival in patients with pN0 ESCC.Patients with ESCC who underwent R0 esophagectomy in the Department of Thoracic Surgery of Sichuan Cancer Hospital from January 2008 to December 2013 were enrolled. Patients were divided into two groups: a surgery alone (Group S) group or a surgery + adjuvant therapy (Group S + A) group. The primary outcomes were overall survival (OS) and disease-free survival (DFS), and every consecutive case was followed up until death or the last follow-up.A total of 387 patients with ESCC patients who had pN0 were enrolled in the study. After propensity score matching (PSM), each group consisted of 150 patients. In the overall cohort, the 5-year OS (75.6% vs. 69.7%; P=0.004) and 5-year DFS (64.9% vs. 48.2%; P=0.003) rates were higher in Group S + A than in Group S. In the matched samples, the same outcomes were observed (5-year OS: 75.6% vs. 69.7%, P=0.026; 5-year DFS: 67.6% vs. 69.6%, P=0.036). Multivariate regression analysis indicated that postoperative chemotherapy was associated with longer OS [hazard ratio (HR): 0.622, 95% confidence interval (CI): 0.416-0.928; P=0.02] and DFS (HR: 0.571, 95% CI: 0.390-0.836; P=0.004); in contrast, T3 stage tumors (HR: 1.953, 95% CI: 1.238-3.082; P=0.004) and <15 lymph node dissections (HR: 1.81; 95% CI: 1.238-2.648; P = 0.002) were found to be independent risk factors for pN0 ESCC.Adjuvant therapy, especially chemotherapy, prolonged OS and DFS for patients with ESCC who had pN0 disease. Fewer lymph node dissections and T3 stage tumors were independent risk factors for OS and DFS.
Introduction Natural plants are valuable resources for exploring new bioactive compounds. Artemisia vulgaris L. is a traditional Chinese medicinal herb that has been historically used for treating multiple diseases. Active compounds isolated and extracted from A. vulgaris L. typically possess immunomodulatory and anti-inflammatory properties. Artemvulactone E (AE) is a new sesquiterpene lactone isolated and extracted from A. vulgaris L. with unclear biological activities. Methods The immunoregulatory effects of AE on macrophages were assessed by ELISA, RT-qPCR, immunofluorescence, and western blot assay. The effect of AE on lipopolysaccharide (LPS) -relates signaling pathways was examined by western blot assay. In zebrafish models, the larvae were yolk-microinjected with LPS to establish inflammation model and the effect of AE was evaluated by determining the survival rate, heart rate, yolk sac edema size, neutrophils and macrophages infiltration of zebrafish. The interaction between AE and Toll-like receptor 4 (TLR4) was examined by molecular docking and dynamic stimulation. Results AE reduced the expression and secretion of pro-inflammatory cytokines (TNF-α and IL-6), inflammatory mediators iNOS and COX-2, as well as decreases the production of intracellular NO and ROS in LPS-stimulated macrophages. In addition, AE exerted its anti-inflammatory effect synergistically by inhibiting MAPK/JAK/STAT3-NF-κB signaling pathways. Furthermore, AE enhanced the survival rate and attenuated inflammatory response in zebrafish embryos treated with LPS. Finally, the molecular dynamics results indicate that AE forms stable complexes with LPS receptor TLR4 through the Ser127 residue, thus completely impairing the subsequent activation of MAPK-NF-κB signaling. Conclusion AE exhibits notable anti-inflammatory activity and represents as a potential agent for treating inflammation-associated diseases.
Background The clinical pathology of gastric signet-ring cell carcinoma (SRC) is still unclear. This meta-analysis was performed to evaluate the difference in biological behavior and prognosis between SRC and non-signet ring cell carcinoma (NSRC). Methods A total of 58 eligible studies were analyzed using RevMan and other auxiliary software. Biological behaviors were compared based on odds ratio (OR) and mean difference (MD). Hazards ratio (HR) was calculated for prognosis based on Kaplan–Meier curves. Results Totally, 28,946 SRC patients were compared with 81,917 NSRC patients. Compared with NSRC patients, lower male: female ratio (OR = 0.53, P < 0.01), younger age (MD = −4.89, P < 0.01), more middle location (OR = 1.64, P < 0.01), more depressed type at early stage (OR = 1.31, P < 0.05), higher incidence of Borrmann type IV (OR = 1.96, P < 0.01), less lymph node metastasis at early stage (OR = 0.78, P < 0.05), better prognosis at early stage (HR = 0.59, P < 0.01), and worse prognosis at advanced stage (HR = 1.19, P < 0.01) were associated with SRC patients. Conclusion The prognosis of SRC at early stage is better than other types of gastric cancer, while that of SRC at advanced stage is relatively poorer.
Objective:To study the value of 1HMRS in brain infarction.Methods: 1HMRS was performed in 24 patients.The influence of different sequences on SNR?width at 50% and ratios of metabolites were investigated.Results:There was significant difference between the group of normal and infarction in NAA/Cho?Cho/Cr?Glx/Cr?Lac/Cr,the significances were less than 0.05.Conclusion:Long echo is better in viewing Lac than short echo.
Background lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and is associated with the development of breast cancer. However, the role of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells ,remain largely unknown. Methods We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods.Then,We evaluated the effects of HOTAIR and miRNA-1 expression on the biological behavior of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, Clonogenic assays, Transwell assay and Flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally,The target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by Luciferace reports. Results The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues（P＜0.05）. Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer（P＜0.0001）. We also verified the existence of paired sequences between miR-1 and HOTAIR, GOLPH3 and miR-1 by Luciferace reports (P＜0.001）. Conclusions The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited cell proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells.
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