Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed in rice (Oryza sativa) fields. It was suggested that the resistance to two classes of acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities of herbicide-metabolizing cytochrome P450. We investigated BSM metabolism in the resistant (R) and susceptible (S) lines of E. phyllopogon, which were originally collected from different areas in California. R plants metabolized BSM through O-demethylation more rapidly than S plants. Based on available information about BSM tolerance in rice, we isolated and analyzed P450 genes of the CYP81A subfamily in E. phyllopogon. Two genes, CYP81A12 and CYP81A21, were more actively transcribed in R plants compared with S plants. Transgenic Arabidopsis (Arabidopsis thaliana) expressing either of the two genes survived in media containing BSM or PX at levels at which the wild type stopped growing. Segregation of resistances in the F2 generation from crosses of R and S plants suggested that the resistance to BSM and PX were each under the control of a single regulatory element. In F6 recombinant inbred lines, BSM and PX resistances cosegregated with increased transcript levels of CYP81A12 and CYP81A21. Heterologously produced CYP81A12 and CYP81A21 proteins in yeast (Saccharomyces cerevisiae) metabolized BSM through O-demethylation. Our results suggest that overexpression of the two P450 genes confers resistance to two classes of acetolactate synthase inhibitors to E. phyllopogon. The overexpression of the two genes could be regulated simultaneously by a single trans-acting element in the R line of E. phyllopogon.
<p>The Antarctic ice mass loss is accelerating due to recent global warming. Changes in Antarctic ice mass have been observed as the gravity change by GRACE (Gravity Recovery and Climate Experiment) satellites. However, the gravity signal includes both the component of the ice mass change and the component of the solid Earth response to surface mass change (Glacial Isostatic Adjustment, GIA). Evaluating the GIA-induced gravity change requires viscoelastic Earth structure and ice history from the last deglaciation.</p><p>Antarctica is characterized by lateral heterogeneity of seismic velocity structure. West Antarctica shows relatively low seismic velocities, suggesting low viscosity regions in the upper mantle. On the other hand, East Antarctica shows relatively high seismic velocities, suggesting thick lithosphere. Here we examine the sensitivities of GIA-induced gravity change in Antarctica to upper mantle viscosity and lithosphere thickness using spherically symmetric Earth models.</p><p>Results indicate that the gravity field change depends on both the upper mantle viscosity profile and the lithosphere thickness. In particular, the long-wavelength gravity field changes become dominant in the adoption of viscoelastic models with a low viscosity layer beneath the elastic lithosphere. The same trend is also shown in the adoption of viscoelastic models with a thick lithosphere, and there is a trade-off between the structure of the low viscosity layer and the thickness of the lithosphere. This trade-off may reduce the effect of the lateral variations in Earth structure beneath Antarctica on the estimate of Antarctic ice sheet mass change.</p>
