Jian Hui Too

National Dental Centre of Singapore

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Raymond A. Smith

Institute of Medical Biology

Simon M. Cool

Vlaams Instituut voor Biotechnologie

Na Yu

National Dental Centre of Singapore

Swee‐Hin Teoh

Nanyang Technological University

Daryl Jimian Lin

National University of Singapore

Bach Quang Le

Bioprocessing Technology Institute

TC Tan

Ammar Mansoor Hassanbhai

National University of Singapore

Luvita Suryani

National University of Singapore

Ning Yu

Yanshan University

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National University of Singapore

Nanyang Technological University

Agency for Science, Technology and Research

Institute of Molecular and Cell Biology

Institute of Medical Biology

University of Queensland

University of Twente

National Dental Centre of Singapore

Duke-NUS Medical School

National Skin Centre

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Affinity-selected heparan sulfate collagen device promotes periodontal regeneration in an intrabony defect model in Macaca fascicularis

Scientific Reports (2023)

Xiaoman Luo Chau Sang Lau Bach Quang Le Tuan Chun Tan Jian Hui Too

Abstract It is challenging to regenerate periodontal tissues fully. We have previously reported a heparan sulfate variant with enhanced affinity for bone morphogenetic protein-2, termed HS3, that enhanced periodontal tissue regeneration in a rodent model. Here we seek to transition this work closer to the clinic and investigate the efficacy of the combination HS3 collagen device in a non-human primate (NHP) periodontitis model. Wire-induced periodontitis was generated in ten Macaca fascicularis, and defects were treated with Emdogain or collagen (CollaPlug) loaded with (1) distilled water, (2) HS low (36 µg of HS3), or (3) HS high (180 µg of HS3) for 3 months. At the endpoint, microscopic assessment showed significantly less epithelial down-growth, greater alveolar bone filling, and enhanced cementum and periodontal ligament regeneration following treatment with the HS-collagen combination devices. When evaluated using a periodontal regeneration assessment score (PRAS) on a scale of 0–16, collagen scored 6.78 (± 2.64), Emdogain scored 10.50 (± 1.73) and HS low scored 10.40 (± 1.82). Notably, treatment with HS high scored 12.27 (± 2.20), while healthy control scored 14.80 (± 1.15). This study highlights the efficacy of an HS-collagen device for periodontal regeneration in a clinically relevant NHP periodontitis model and warrants its application in clinical trials.

Periodontal fiber

Clinical attachment loss

Debridement (dental)

10.1038/s41598-023-38818-y

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Application of a BMP2-binding heparan sulphate to promote periodontal regeneration

European Cells and Materials (2021)

BQ Le Jian Hui Too TC Tan Raymond A. Smith Victor Nurcombe

Periodontitis is the most common inflammatory disease that leads to periodontal defects and tooth loss. Regeneration of alveolar bone and soft tissue in periodontal defects is highly desirable but remains challenging. A heparan sulphate variant (HS3) with enhanced affinity for bone morphogenetic protein-2 (BMP2) that, when combined with collagen or ceramic biomaterials, enhances bone tissue regeneration in the axial and cranial skeleton in several animal models was reported previously. In the current study, establishing the efficacy of a collagen/HS3 device for the regeneration of alveolar bone and the adjacent periodontal apparatus and related structures was sought. Collagen sponges loaded with phosphate-buffered saline, HS3, BMP2, or HS3 + BMP2 were implanted into surgically-created intra-bony periodontal defects in rat maxillae. At the 6 week end- point the maxillae were decalcified, and the extent of tissue regeneration determined by histomorphometrical analysis. The combination of collagen/HS3, collagen/BMP2 or collagen/HS3 + BMP2 resulted in a three to four-fold increase in bone regeneration and up to a 1.5 × improvement in functional ligament restoration compared to collagen alone. Moreover, the combination of collagen/HS3 + BMP2 improved the alveolar bone height and reduced the amount of epithelial growth in the apical direction. The implantation of a collagen/ HS3 combination device enhanced the regeneration of alveolar bone and associated periodontal tissues at amounts comparable to collagen in combination with the osteogenic factor BMP2. This study highlights the efficacy of a collagen/HS3 combination device for periodontal regeneration that warrants further development as a point-of-care treatment for periodontitis-related bone and soft tissue loss.

Periodontal fiber

10.22203/ecm.v042a10

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Effects of Electromagnetic Field on Proliferation, Differentiation, and Mineralization of MC3T3 Cells

Tissue Engineering Part C Methods (2019)

Luvita Suryani Jian Hui Too Ammar Mansoor Hassanbhai Feng Wen Daryl Jimian Lin

We present the study about how the parameters of pulsed electromagnetic field (PEMF) stimulus affected calvarial osteoblast precursor cell in terms of growth, viability, and differentiation. This research provides insight and foundation to clinical application of noninvasive therapy using PEMF to improve bone regeneration.

Von Kossa stain

10.1089/ten.tec.2018.0364

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Citations (26)