Farhad Ghadiri Soufi

Hormozgan University of Medical Sciences

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Mohammad Reza Alipour

Mashhad University of Medical Sciences

Iraj Salehi

Hamedan University of Medical Sciences

Ebrahim Eftekhar

Hormozgan University of Medical Sciences

Nasser Ahmadiasl

Tabriz University of Medical Sciences

Kamila Kamali

Islamic Azad University, Larestan Branch

Hamid Ahmadieh

Shahid Beheshti University of Medical Sciences

Daryoush Mohammadnejad

Tabriz University of Medical Sciences

Mohsen Alipour

Zanjan University of Medical Sciences

Rafigheh Ghiassie

Y Doustar

Islamic Azad University of Tabriz

Cooperative Institutions

Tabriz University of Medical Sciences

Hormozgan University of Medical Sciences

University of Tabriz

Tehran University of Medical Sciences

Shahid Beheshti University of Medical Sciences

Iran University of Medical Sciences

Shahid Beheshti University

Biotechnology Research Center

Islamic Azad University of Tabriz

Kerman University of Medical Sciences

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Hyperglycemia- Induced NF-κB Activation Increases microRNA-146a Expression in Human Umbilical Vein Endothelial Cells

Journal of Ardabil University of Medical Sciences (2019)

Elnaz Salmani Korjan Kamila Kamali Majid Katebi Afshin Samiei Farhad Ghadiri Soufi

Background & objectives: Nuclear Factor kappa B (NF-κB), a master switch transcription factor, plays a critical role in the progression and development of hyperglycemia-induced microangiopathy.Hyperglycemia activates NF-κB, and subsequently increases proinflammatory cytokines such as TNF-α, IL-6 and IL-1β leading to development of inflammation.Some new studies have revealed the involvement of microRNA-146a (miR-146a) in the pathogenesis of diabetic complications through an NF-κB-dependent negative feedback loop manner.Despite numerous reports indicating changes of plasma miR-146a during hyperglycemia, the origin of this change remains unclear.This study was designed to evaluate the role of NF-κB on the miR-146a gene expression level in human umbilical vein endothelial cells (HUVECs) during a hyperglycemic condition.Methods: HUVECs were cultured in normal glucose (5 mmol/L), and hyperglycemic (25 mmol/L) endothelial cell growth medium in the six well plates for 24 h.JSH-23 (30 μmol/L), as an inhibitor of NF-κB translocation to the nucleus, was added to the culture medium, 30 min before induction of hyperglycemia.Quantitative Real Time PCR was performed to measure the expression levels of miR-146a and mRNA NF-κB.NF-κB activity was measured by Elisa.Results: Hyperglycemia markedly increased the NF-κB activity and mRNA level in HUVECs.The expression of miR-146a significantly increased in hyperglycemic group compared to the normoglycemic group.On the other hand, JSH-23 prevented from miR-146a increment in hyperglycemic group and also it increased the mRNA expression level of NF-κB in this group. Conclusion:This result shows that NF-κB increases the gene expression of miRNA-146a in the early phase of hyperglycemia in HUVECs.

Proinflammatory cytokine

Pathogenesis

10.29252/jarums.19.1.7

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Influance of regular swimming on serum levels of CRP, IL-6, TNF-α in high-fat diet-induced type 2 diabetic rats

General Physiology and Biophysics (2016)

Rafighe Ghiasi Farhad Ghadiri Soufi Gisou Mohaddes Alireza Alihemmati Mohammad Hossein Somi

Due to key role of inflammation in pathogenesis of type 2 diabetes mellitus (T2DM), aim of this study was evaluating the influance of regular swimming on serum levels of C-reactive protein (CRP), interlukin-6 (IL-6), tumor necrosis factor-α (TNF-α) in high-fat diet-induced diabetic rats. Fourty male Wistar rats were randomly divided into control, diabetic, exercise and diabetic-exercise groups (n = 10). Diabetes was induced by high-fat diet and streptozotocin (35 mg/kg, i.p.). In exercise groups, after induction of diabetes, animals were subjected to swimming (60 min/5 days a week) for 10 weeks. At the end of training, rats were anestatized and blood samples and pancreatic tissues were collected and used for evaluation of CRP, IL-6, TNF-α and pancreatic histopatholology. Our results showed significantly increase in lymphocytes, monocytes and decrease in neutrophils in diabetic rats (p < 0.01), which these parameters significantly reversed to control levels by induction of swimming (p < 0.01). In diabetic group, the levels of CRP, IL-6 and TNF-α increased (p < 0.01), and swimming decreased these factors significantly. Histopathological results of this study also showed that swimming can prevent damage induced by diabetes. The present study indicates that swim training is associated with improved inflammation and inflammatory mediators and pancreatic damage.

Pathogenesis

10.4149/gpb_2016007

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Attenuation of phosphorylated connexin-43 protein levels in diabetic rat heart by regular moderate exercise.

PubMed (2014)

Farzam Sheikhzadeh Hesari Nazli Khajehnasiri Seyed Mahdi Banan Khojasteh Farhad Ghadiri Soufi Ali Dastranj Tabrizi

High blood glucose levels increase the ratio of phosphorylated to non-phosphorylated connexin-43 amounts, which leads to the decomposition of the hyperphosphorylated connexin-43. This can cause heart arrhythmia in diabetic patients. Considering the effective role of exercise in diabetic patients, and because there are few studies regarding the effect of exercise on phosphorylated connexin-43 protein levels, in the present study the impact of different periods of moderate regular exercise on phosphorylated Connexion-43 levels were examined.Sixty (60) male Wistar rats (300 ± 50 g) were randomly divided into six groups (n = 10). A week after induction of diabetes by injection of streptozotocin, one hour treadmill exercise, 5 days a week with 22 (m/min) speeds was undertaken. Left ventricles of hearts were isolated and immediately frozen. Finally, phosphorylated connexin-43 protein levels were measured by ELISA method.The means of blood glucose levels were significantly decreased (P < 0/05) by increasing days of exercise. The means of blood glucose levels were significantly decreased (P < 0/05) by increasing days of exercise. Regular moderate exercise reduced the connexin-43 levels by increasing days of exercise (P < 0.01).It is concluded that regular moderate exercise reduces the amount of phosphorylated connexin-43 protein levels in the ventricular myocardium, by reducing blood glucose levels. This can result in partial inhibition of cardiac arrhythmia observed in diabetic patients. This research was done in Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

Treadmill

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Long-term resveratrol administration improves diabetes-induced pancreatic oxidative stress, inflammatory status, and β cell function in male rats

Brazilian Journal of Pharmaceutical Sciences (2023)

Samin Nahavandi Masoumeh Rahimi Mohammad Reza Alipour Farhad Ghadiri Soufi

Diabetes is a metabolic disorder caused by insulin resistance or a defect in the pancreatic beta cells in insulin secretion. The aim of this study was to evaluate the possible effectiveness of long-term administration of resveratrol on inflammatory and oxidative stress markers in the pancreatic tissue of diabetic rats. Male Wistar rats (n = 24) were randomly divided into four groups of six animals, namely a healthy group, a healthy group receiving resveratrol, a diabetic control group, and a diabetic group receiving resveratrol. Diabetes was induced by single dose injection of streptozotocin (50 mg/kg; ip), 15 min after injection of nicotinamide (110 mg/kg; ip). Resveratrol was also administered by gavage (5 mg/kg/day) for 4 months. Administration of resveratrol alleviated hyperglycemia, weight loss and pancreatic β cell function measured by HOMA-β. Resveratrol improved oxidative stress (nitrate/nitrite, 8-isoprostane and glutathione) and proinflammatory markers (tumor necrosis factor α, cyclooxygenase 2, interleukin 6 and nuclear factor kappa B) in the pancreatic tissue of diabetic rats. Resveratrol administration had no significant effect on the activity of superoxide dismutase and catalase enzyme. These observations indicate that resveratrol administration may be effective as a beneficial factor in improving pancreatic function and reducing the complications of diabetes.

Proinflammatory cytokine

10.1590/s2175-97902023e21468

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The Efficacy of Long-Term Resveratrol Administration on the Activity of Cyclooxygenase 1 and 2 and Nuclear Factor Kappa B in Type 2 Diabetic Rat Kidney

Medical Journal of Tabriz University of Medical Sciences (2013)

Saeed Khameneh Farhad Ghadiri Soufi Mohammad Reza Alipour Fatemeh Afshar Fariba Mirzaei

Background and Objectives: There are several studies conducted to investigate the pathogenesis of diabetic nephropathy, but the obtained results are controversial. It remains one of the most important reasons of mortality in diabetic subjects. Previous researches have indicated that cyclooxygenase 2 (COX2) has an important role in the pathogenesis of diabetic nephropathy. Additionally resveratrol is an herbal polyphenol and it has been beneficial anti-inflammatory effects during short-term administration. This study aimed to investigate the efficacy of long-term resveratrol administration on the activity of COX1, COX2 and nuclear factor kappa B (NF-κB) in diabetic Rats. Materials and Methods: Twenty four male Wistar rats (320-340 g) were randomly divided into four groups (n=6 for each group): normal control, diabetic control, normal rats treated with resveratrol, and diabetic rats treated with resveratrol. Diabetes was induced by injection of streptozotocin (50 mg/kg; i.p.), 15 min after the prescription of nicotinamide (110 mg/kg; i.p.) and resveratrol (5 mg/kg/day) was gavaged for four months. At the end of protocol, blood glucose, insulin, urea and creatinine as well as the activity of renal COX1, COX2 and NF-κB were measured. Results: Four-month resveratrol administrations significantly attenuated the enhancement of blood glucose, urea and kidney to body weight ratio in diabetic rats (p <0.05 for all). Moreover, long-term resveratrol administration to diabetic rats reduced renal COX2 and NF-κB activities. Conclusion: Since the efficient control of blood glucose has a key role in preventing of diabetes complications and in regard to the anti-inflammatory effects of resveratrol in kidneys. It could be considered as a supplementation in preventing of diabetes renal complications.

Blood urea nitrogen

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Effect of Regular Swimming on Oxidative Stress and Atherogenic Index in Blood of Diabetic Male Rats

DOAJ (DOAJ: Directory of Open Access Journals) (2007)

Iraj Salehi Mahdi Mohammadi Safar Farajnia Farhad Ghadiri Soufi Reza Badalzadeh

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MicroRNA-146a and its adapter proteins are affected by diabetes in rat’s heart

Bratislavské lekárske listy/Bratislava medical journal (2016)

Ebrahim Eftekhar Maryam Doustaki Zaboli Majid Katebi Farhad Ghadiri Soufi

OBJECTIVES: This study was conducted to explore whether microRNA-146a and its adapter proteins (TNF-α receptor-associated factor 6 (TRAF6) and interleukin-1 receptor-associated kinase 1 (IRAK1)) are affected by diabetes in the rat heart.METHODS: Twelve male Sprague-Dawley rats were randomized into control and diabetic groups (n = 6).Streptozotocin-nicotinamide experimental model was used to induce type 2 diabetes.The gene expression of MicroRNA-146a, nuclear factor-κB (NF-κB), IRAK1 and TRAF6, as well as NF-κB activity, IRAK1 and TRAF6 protein levels were measured.Moreover, NF-κB activity was measured in response to miR-146a mimic transfection (20 nmol) in human umbilical vein endothelial cells (HUVECs) under hyperglycemic condition (25 mM D-glucose for 24 h).RESULTS: The expression of MicroRNA-146a was increased in the heart tissue, 2 months after diabetes induction and in HUVECs.Also, the mRNA and protein levels of NF-κB, IRAK1 and TRAF6 were increased in the heart of diabetic rats.Moreover, transfection of miR-146a mimic prevented from a signifi cant increase of NF-κB activity in hyperglycemic HUVECs.CONCLUSION: Presumably, a defect in the regulation of IRAK1 and TRAF6 can weaken miR-146a regulatory effect and provides a situation for sustained activation of NF-κB and its targets to promote cardiac cells toward abnormalities (Fig. 3, Ref. 28).

10.4149/bll_2016_031

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Anti‐inflammatory properties of resveratrol in the retinas of type 2 diabetic rats

Clinical and Experimental Pharmacology and Physiology (2014)

Farhad Ghadiri Soufi Elham Arbabi‐Aval Mozhgan Rezaei Kanavi Hamid Ahmadieh

Resveratrol (trans-3,5,4'-trihydroxystilbene) is a nutritional supplement with anti-inflammatory properties. The present study investigated the long-term anti-inflammatory property of resveratrol in the retinas of type 2 diabetic rats. Male Wistar rats were divided into four groups: normal control, diabetic control, resveratrol-treated normal rats and resveratrol-treated diabetic rats. Type 2 diabetes was induced by a single dose injection of streptozotocin (50 mg/kg; i.p.) 15 min after the administration of nicotinamide (110 mg/kg; i.p.) in 12-h fasted rats (the streptozotocin-nicotinamide type 2 diabetic model). Oral resveratrol administration (5 mg/kg per day for 4 months) significantly improved glucose tolerance, and alleviated hyperglycemia and weight loss in diabetic rats. Furthermore, resveratrol administration significantly decreased the elevated levels of nuclear factor-κB activity, and mRNA expression, tumour necrosis factor alpha level and apoptotic cells in the retinas of the diabetic rats. Furthermore, resveratrol did not significantly affect plasma insulin levels. Long-term resveratrol administration has beneficial anti-inflammatory properties in a rat model of diabetes. However, whether resveratrol exerts its effects directly or through reducing blood glucose levels requires further investigation.

10.1111/1440-1681.12326

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The association of sleep duration and metabolic syndrome in the Bandare-Kong cohort study, a cross-sectional survey (finding from PERSIAN cohort study)

Diabetology & Metabolic Syndrome (2021)

Shideh Rafati Maryam Isheh Abnoos Azarbad Farhad Ghadiri Soufi Arash Rahimi

A variety of health problems, such as metabolic syndrome (MetS), have been linked to sleep disorders. While numerous epidemiological studies have shown a U-shaped relationship between sleep duration and poor health outcomes, the results were limited and inconsistent. This study was designed to evaluate the relationship between sleep duration and MetS.This population-based study was conducted on the participants aged 35-70 of Bandare-Kong Non-Communicable Diseases (BKNCD) Cohort Study, a part of Prospective Epidemiological Research Studies in IrAN (PERSIAN). MetS was diagnosed according to the National Cholesterol Education Program (NCEP) criteria and the Iranian-specific cut-off for waist circumference (≥ 95 cm). Sleep information was extracted through a standard questionnaire based on self-reported information. Data were analyzed by R software using generalized additive models (GAMs). A statistically significant level was considered as P < 0.05.A total of 3695 participants were included in the analyses. The mean age was 48.05 years (SD 9.36), and 2067 (55.9%) were female. The estimated Prevalence of MetS was 35.9%, and women appeared to be more likely to have MetS than men (P < 0.001). There was a non-linear and linear association between sleep duration and the risk of MetS in women and men, respectively. The lowest risk was observed among those with 7-7.5 h of sleep duration per night.Long sleep duration was associated with increased risk of MetS and higher MetS severity score in both genders, while the short sleep duration increased the risk of Mets as well as MetS severity score just in women. The longitudinal studies would be suggested to assess the relationship between sleep quality and quantity components and MetS.

Cross-sectional study

10.1186/s13098-021-00737-1

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Role of four-week resistance exercise in preserving the heart against ischaemia-reperfusion-induced injury : cardiovascular topic

Cardiovascular journal of South Africa/Cardiovascular journal of Southern Africa (2012)

Y Doustar Farhad Ghadiri Soufi Afshar Jafary Mohaddeseh Mahmoudi Saber Rafigheh Ghiassie

Objective: We studied the cardioprotective effect of resistance training against ischaemia-reperfusion-induced injury.Methods: Forty male rats were divided into trained and sedentary groups (n = 20 for each).The trained rats were exercised at 12 repetitions/set, four sets/day and five days/ week for four weeks.Transient regional ischaemia of the left anterior descending coronary artery (40 min) was followed by 80 min of reperfusion.Results: Baseline developed and diastolic pressures and coronary flow were similar in the two groups.While diastolic pressure increased and developed pressure and coronary flow decreased in both the ischaemic and perfusion periods (as indices of cardiac damage), there were no statistically significant differences between the trained and sedentary groups in these parameters.Resistance training did not significantly change the infarct size and apoptosis rate.Conclusion: We did not see a cardioprotective effect of resistance exercise against ischaemia-reperfusion-induced injury in this study.A precise conclusion about this issue needs more investigations.

10.5830/cvja-2012-050

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