Andrea Lauková

Slovak Academy of Sciences

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Monika Pogány Simonová

Slovak Academy of Sciences

114

Viola Strompfová

Slovak Academy of Sciences

109

Iveta Plachá

Institute of Animal Physiology of the Slovak Academy of Sciences

Anna Kandričáková

Slovak Academy of Sciences

ĽC

Ľubica Chrastinová

National Agricultural and Food Centre

JŠ

Jana Ščerbová

Slovak Academy of Sciences

Renáta Szabóová

University of Veterinary Medicine in Košice

M. Chrenková

National Agricultural and Food Centre

Zuzana Formelová

National Agricultural and Food Centre

ĽC

Ľ. Chrastinová

National Agricultural and Food Centre

Cooperative Institutions

University of Veterinary Medicine in Košice

Slovak Academy of Sciences

Institute of Animal Physiology of the Slovak Academy of Sciences

University of Veterinary Medicine Vienna

Bioscience (Slovakia)

University of Veterinary Medicine

Animal Production Research Centre

University of Pavol Jozef Šafárik

Institute of Parasitology of the Slovak Academy of Sciences

National Agricultural and Food Centre

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Enterococcal Species Associated with Slovak Raw Goat Milk, Their Safety and Susceptibility to Lantibiotics and Durancin ED26E/7

Processes (2021)

Andrea Lauková Valentína Focková Monika Pogány Simonová

Goat milk has become a popular item of human consumption due to its originality. Enterococci are ubiquitous bacteria, and they can also be found in traditional dairy products. This study focuses on the safety of enterococci from Slovak raw goat milk and on their susceptibility to lantibiotic bacteriocins and durancin ED26E/7, which has not previously been studied. Biofilm formation ability in enterococci, virulence factor genes, enzyme production and antibiotic profile were investigated. Samples of raw goat milk (53) were collected from 283 goats in Slovakia. MALDI-TOF mass spectrometry identified three enterococcal species: Enterococcus faecium, E. hirae and E. mundtii, with dominant occurrence of the species E. faecium. Low-grade biofilm formation ability (0.1 ≤ A570 < 1.0) was found in four strains of E. faecium. Gelatinase, hyaluronidase, aggregation substance and enterococcal surface protein genes were absent in these enterococci. Gene efaAfm (adhesin) was detected in five E. faecium strains. However, it was not detected in biofilm-forming strains. Enterococci detected in Slovak raw goat milk were found not to have pathogenic potential; four strains even produced high amounts of useful β-galactosidase. The strains were susceptible to lantibiotic bacteriocin treatment and to durancin ED26E/7 as well, which represents original information in dairy production.

Enterococcus faecium

Lantibiotics

10.3390/pr9040681

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Citations (11)

Trouts-Derived, Gram-Negative Microbiota from Slovakian Water Sources and Their Relation to Postbiotics

Andrea Lauková Anna Kandričáková Jana Ščerbová Monika Pogány Simonová R. Žitňan

Gram

10.2139/ssrn.5148437

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Citations (0)

Effect of probiotic bacteria on phagocytosis and respiratory burst activity of blood polymorphonuclear leukocytes (PMNL) in mice infected with Trichinella spiralis

Veterinary Parasitology (2016)

E. Dvorožňáková B. Bucková Zuzana Hurníková Viera Révajová Andrea Lauková

Trichinella spiralis

Respiratory burst

Lactobacillus fermentum

Enterococcus faecium

10.1016/j.vetpar.2016.07.004

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Citations (42)

Benefficial effect of plant extracts in rabbit husbandry

Acta Veterinaria Brno (2012)

Renáta Szabóová Andrea Lauková Ľubica Chrastinová Viola Strompfová Monika Pogány Simonová

The present study evaluates the effect of plant extracts of oregano and commercial Xtrakt TM administrations on microbial, biochemical, immunological and nutritional indicators and on Eimeria sp. oocyst occurrence in rabbits. Rabbits (5 weeks old, Hy-plus hybrid, n = 66) were divided into experimental group 1 (E1) with oregano extract application, experimental group 2 (E2) with Xtract TM application and control group. Natural substances were administered for the first 21 days. The experiment lasted for 42 days. The antibacterial effect was determined by the decrease of coagulase-positive staphylococci in E1 compared to control at day 42. Staphylococcus aureus cells were detected in lower counts in E2 compared to control at day 21. The counts of Clostridium- like bacteria were lower in both experimental groups at day 21 compared to day 7 (difference 1.2 and 1.3 log cycles, respectively) and to control (difference 0.5 and 0.3 log cycles, respectively). At day 7, the counts of coliforms in E1 were significantly lower than in E2 ( P < 0.01). In rabbits fed with oregano, reduction of Eimeria sp. oocysts and higher value of phagocytic activity (21.6 ± 0.51 %) were found compared to Xtract TM , and prolonged immuno-stimulatory effect was noted. In the animals of both experimental groups higher final weight, feed conversion ratio and lower mortality were achieved compared to control. The administration of oregano showed antibacterial, anticoccidial, and immunomodulatory effects. The results showed that oregano administration may be used as an alternative prophylactic measure in rabbits.

Negative control

10.2754/avb201281030245

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Citations (18)

Bacteriocin Activity of Enterococci from Rabbits

Veterinary Research Communications (2007)

Monika Pogány Simonová Andrea Lauková

Enterococcus faecium

Strain (injury)

Molecular mass

10.1007/s11259-006-3411-4

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Citations (36)

Virulence factor genes possessing Enterococcus faecalis strains from rabbits and their sensitivity to enterocins

World Rabbit Science (2017)

Monika Pogány Simonová Andrea Lauková

Information concerning the virulence factor genes and antibiotic resistance of rabbit enterococci is limited, so in this study we tested the virulence factor genes in Enterococcus faecalis strains from rabbits. Moreover, their resistance/sensitivity to antibiotics and sensitivity to enterocins was also tested, with the aim of contributing to our enterocin spectra study and to indicate the possibility of enterocin application in prevention or contaminant elimination in rabbit husbandry. A total of 144 rabbit samples were treated using a standard microbiological method. Thirty-one pure colonies of the species Enterococcus faecalis were identified, using the MALDI-TOF identification system and confirmed using phenotyping, among which 15 strains were virulence factor gene absent. The gelE gene was the most detected (42%); however, the expression of gelatinase phenotype did not always correlate with the detection of gelE. Strains did not show ß-haemolysis and were mostly resistant to tested antibiotics, but sensitive to enterocins (Ent), mainly to Ents EK13=A (P), 2019 and Ent M. Rabbit E. faecalis strains displayed antibiotic resistant traits and the presence of expressed and silent virulence genes, but they showed high levels of sensitivity to natural antimicrobials-enterocins, which indicates the possible prevention of multidrug and virulent enterococcal contaminants by enterocins.

Virulence factor

Enterococcus faecalis

Enterococcus faecium

10.4995/wrs.2017.5694

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Citations (3)

The Bacteriocin-Producing Strain Lacticaseibacillus Paracasei LPa 12/1 from Raw Goat Milk, a Potential Additive in Dairy Products

Andrea Lauková E. Dvorožňáková Miroslava Vargová Jana Ščerbová Valentína Focková

Goat milk starts to be gradually the preferred milk by consumers worldwide and also in Slovakia. It is also demanded as functional and/or nutraceutical drink as it is rich in bioactive components. Goat milk contains complex of microbiota among which the phylum Firmicutes have been occurred with abundance 20.5 %. Among individual representatives of this phylum are involved also lacticaseibacilli. This study has been focused on bacteriocin-producing, beneficial strain Lacticaseibacillus paracasei LPa 12/1 isolated from raw goat milk and its potential to be additive in dairy products. Based on safety testing of bacteriocin-producing LPa 12/1 strain using BALB/c mice, it looks as safe. Even increase tendency in phagocytic activity in blood of mice was noted after LPa 12/1 strain application. Encapsulated form of LPa 12/1 strain producing thermo-stable bacteriocin seems to be a suitable to supplement dairy products. The strain colonized better ewe-goat milk yoghurt (up to 6.1 cfu/g log 10) than cow milk yoghurt (almost 5.0 cfu/g log 10). Although specific organoleptic tests were not involved, cow milk yoghurts remained in better consistency after LPa 12/1 strain supplementation in comparison with ewe-goat milk yoghurts. LPa 12/1 strain may be supposed as a new potential additive in dairy products/yoghurts.

Strain (injury)

Organoleptic

Nutraceutical

Dairy industry

Cow milk

10.20944/preprints202308.1428.v1

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Citations (2)

Potential Applications of Probiotic, Bacteriocin-Producing Enterococci and Their Bacteriocins

CRC Press eBooks (2011)

Andrea Lauková

10.1201/b11503-4

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Citations (4)

USE OF BACTERIOCIN PREPARATION WITH ANTI-MICROBIAL ACTIVITY IN SAINT-PAULIN CHEESE

Bulletin of the Veterinary Institute in Pulawy (2003)

Andrea Lauková Geertrui Vlaemynck

The growth, bacteriocin production and stability of Enterococcus faecium CCM4231 strain in milk and cheese were investigated. Moreover, antagonistic effect of bacteriocin preparation (containing bacteriocin-enterocin CCM4231) was studied in Saint-Paulin cheese experimentally contaminated with Listeria monocytogenes Ohio. The growth of E. faecium CCM4231 in cheese milk and cheese reached 10.1 ± 0.1 log10 cfu/ml/g. Production of bacteriocin started after 4 h from the beginning of CCM4231 strain cultivation and culminated after 8 h with activity in the range from 200 to 6 400 AU/ml depending on the indicator organism used. This activity was stable or only slightly diminished during 1 or 2 week storage at 4°C. Addition of bacteriocin preparation into cheese vat contaminated with 0.1% inoculum of Listeria monocytogenes resulted in the reduction of this contaminant in cheese during ripening period with the difference 2.7 log cycles between the experimental cheese and the control one. Although, at the end of ripening slight re-growth of L. monocytogenes was noticed in the experimental cheese, our results give chances for the use of natural substance-bacteriocin to prevent, control or reduce the food contaminants. Of course, more studies are still needed to assess the role of bacteriocin or its preparations as food preservatives.

Enterococcus faecium

Source

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Citations (1)

Bioactive Enterococci Isolated from Slovak Ewes’ Lump Cheese

Scientia Agriculturae Bohemica (2016)

Andrea Lauková Viola Strompfová Renáta Szabóová A. Slottová Martin Tomáška

Abstract Enterococci are widespread organisms; some of their properties are desired in dairy industry. They can produce antimicrobial proteinaceous substances (enterocins) linked to food biopreservation. This study focused on bioactive Enterococcus faecium and Enterococcus faecalis strains from Slovak ewes’ lump cheeses to check genes encoding enterocins production and inhibition activity. The total counts of enterococci in ewes’ lump cheeses reached 5.95 ± 2.44 log CFU/g on average. Genotypization by PCR and identification by MALDI-TOF mass spectrometry alloted 12 strains to the species Enterococcus faecium and 18 strains to the species E. faecalis. Enterococci were hemolytic phenotype free. Gelatinase negative strains were tested for the presence of enterocins genes. E. faecium and E. faecalis strains from Slovak ewes’ lump cheeses possessed mostly genes for enterocins P and A. Enterocin gene free E. faecalis EE29E3 inhibited indicator Enterococcus avium EA5 (inhibition zone > 10 mm); EE36E1inhibited Listeria innocua LMG 13568 (inhibiton zone 12 mm). Among E. faecium possessing enerocins genes, inhibition activity was only noted in EF27E4 strain (against E. avium EA5, Listeria monocytogenes CCM4699; inhibiton zone 10–22 mm). E. faecium EF27E4 was selected for more detailed studies in vitro aimed at its potential use in dairy industry.

Enterococcus faecium

Enterococcus faecalis

Biopreservation

10.1515/sab-2016-0027

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Citations (2)