Effect of juvenile hormone activity of juvenile hormone I and several juvenile hormone mimics (JHM) were investigated on cell growth and macromolecular synthesis of an established insect cell line derived from mosquito (Culex molestus) ovary. The ceil growth in medium with 10−4 m methoprene (ZR-515) or 10−4 m farnesol was perfectly inhibited. The cell shape changed into slender or fibloblast like shape. These compounds inhibited also DNA, RNA and protein syntheses. RNA synthesis was most effectively inhibited than others. Methoprene is a stronger inhibitor of RNA and protein syntheses than puromycin or actinomycin D. The cells incubated in medium with methoprene for one day recovered the cell growth 2 weeks after the methoprene treatment and then the cell shape also recovered. RNA synthesis inhibited by methoprene was also recovered 90 min after the methoprene treatment. DNA, RNA and protein syntheses in the cells treated with juvenile hormone were immediately inhibited but the inhibition was reversible.
Effect of juvenile hormone activity of juvenile hormone I and several juvenile hormone mimics (JHM) were investigated on cell growth and macromolecular synthesis of an established insect cell line derived from mosquito (Culex molestus) ovary. The cell growth in medium with 10-4 M methoprene (ZR-515) or 10-4 M farnesol was perfectly inhibited. The cell shape changed into slender or fibloblast like shape. These compounds inhibited also DNA, RNA and protein syntheses. RNA synthesis was most effectively inhibited than others. Metho-prene is a stronger inhibitor of RNA and protein syntheses than puromycin or actinomycin D. The cells incubated in medium with methoprene for one day recovered the cell growth 2 weeks after the methoprene treatment and then the cell shape also recovered. RNA synthesis inhibited by methoprene was also recovered 90min after the methoprene treatment. DNA, RNA and protein syntheses in the cells treated with juvenile hormone were immediately inhibited but the inhibition was reversible.
The lipids profile of Chlorella vulgaris grown on an organic medium and inorganic medium were examined.1) The neutral lipid on the organic medium was 2.8 g/100 g and inorganic medium was 1.88 g/100 g of the total dry weight, which composed of 80% waxesters and 20% triacylglycerols on both medium.2) The phospholipid on the organic medium was 8.6 g/100 g and on the inorganic medium was 6.2 g/100 g of the total dry weight, those composition (PG, PC, PE, PI) on the both medium were similar but those distribution of the fatty acid were different.3) The glycolipid were contained on the both medium about 5.7 g/100 g of the total dry weight, those composition (MGDG, DGDG, SQDG) and fatty acid composition of each glycolipid on the both medium were different. 4) Both hexadecatetraenoic acid (C16 : 4) and octadecatetraenoic acid (C18 : 4) were observed in the glycolipid and phospholipids except PG which was absent, while triacylglycerols were not present.5) Trans-hexadecenoic acid was present only in the PG, which contained 1.6% on organic medium and 2.3% on inorganic medium.
