Gabriela Lorenc–Plucińska

Polish Academy of Sciences

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Krzysztof Stobrawa

Polish Academy of Sciences

Piotr Karolewski

Institute of Dendrology

Andrzej Pluciński

Polish Academy of Sciences

Agata Zemleduch-Barylska

University of Life Sciences in Poznań

Agnieszka Szuba

Institute of Dendrology

H. Ziegler

Technical University of Munich

Paweł Pluciński

University of Bath

Anna Szadel

Polish Academy of Sciences

Jacek Oleksyn

Polish Academy of Sciences

Peter B. Reich

University of Minnesota

Cooperative Institutions

Polish Academy of Sciences

Institute of Dendrology

University of Life Sciences in Poznań

Technical University of Munich

Ludwig-Maximilians-Universität München

Minnesota Department of Natural Resources

University of Minnesota

University of Minnesota System

Twin Cities Orthopedics

Darmstadt University of Applied Sciences

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The effect of atmospheric pollution on sucrose release from the mesophyll symplast

Biological Bulletin of Poznań. Supplement (1995)

Gabriela Lorenc–Plucińska

Symplast

Atmospheric pollution

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How harmful are automobile emissions

Biological Bulletin of Poznań (1998)

Gabriela Lorenc–Plucińska A. Byczynska

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Acclimation of respiratory temperature responses in northern and southern populations of Pinus banksiana

New Phytologist (2008)

Mark G. Tjoelker Jacek Oleksyn Gabriela Lorenc–Plucińska Peter B. Reich

Temperature acclimation of respiration may contribute to climatic adaptation and thus differ among populations from contrasting climates. Short-term temperature responses of foliar dark respiration were measured in 33-yr-old trees of jack pine (Pinus banksiana) in eight populations of wide-ranging origin (44-55 degrees N) grown in a common garden at 46.7 degrees N. It was tested whether seasonal adjustments in respiration and population differences in this regard resulted from changes in base respiration rate at 5 degrees C (R(5)) or Q(10) (temperature sensitivity) and covaried with nitrogen and soluble sugars. In all populations, acclimation was manifest primarily through shifts in R(5) rather than altered Q(10). R(5) was higher in cooler periods in late autumn and winter and lower in spring and summer, inversely tracking variation in ambient air temperature. Overall, R(5) covaried with sugars and not with nitrogen. Although acclimation was comparable among all populations, the observed seasonal ranges in R(5) and Q(10) were greater in populations originating from warmer than from colder sites. Population differences in respiratory traits appeared associated with autumnal cold hardening. Common patterns of respiratory temperature acclimation among biogeographically diverse populations provide a basis for predicting respiratory carbon fluxes in a wide-ranging species.

10.1111/j.1469-8137.2008.02624.x

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Citations (104)

An optimized method to extract poplar leaf proteins for two‐dimensional gel electrophoresis guided by analysis of polysaccharides and phenolic compounds

Electrophoresis (2013)

Agnieszka Szuba Anna Wojakowska Gabriela Lorenc–Plucińska

Commonly used methods for protein extraction from plant leaves, such as extraction with phenol or a combination of TCA and acetone, were ineffective for four tested cultivars of poplar. Moreover, multiple protocols for 2DE of the extracted proteins gave different results when protein profiles of relatively closely related plants were compared. Given that polycyclic compounds strongly hinder 2DE, we analyzed the impact of polyphenols and polysaccharides present in the plant tissues used for protein extraction, on the quality of 2DE protein profiles. Analysis of content of polyphenols and polysaccharides in leaves of poplar cultivars showed that even small differences in concentrations of analyzed metabolites accompany large differences between poplar cultivars when considering the susceptibility of samples to protein extraction for 2DE. High-quality 2DE results were correlated with decreased amounts of polyphenols. Additional analysis using MS/MS suggested that only levels of total phenolics affected the results of 2DE. Soluble total nonstructural carbohydrates also had a negative effect, but the level of starch was not important. Finally, we present an optimized method (OPTI) for extraction of proteins from poplar leaves, which enables reliable comparative analysis of four different poplar cultivars i.e. 'Eridano', 'Villafranca', 'NE-42' and 'Luisa Avanzo', which have not yet been used for the proteomic studies.

Phenol extraction

10.1002/elps.201300223

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Chronic environmental pollution alters adenylate levels in needles and fine roots of Scots pine

Acta Physiologiae Plantarum (2003)

Gabriela Lorenc–Plucińska Agnieszka Byczyñska Andrzej Pluciński

Energy charge

Scots pine

Adenine nucleotide

10.1007/s11738-003-0032-1

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Sulfite inhibition of sucrose transport into vacuoles of cottonwood leaves

Acta Societatis Botanicorum Poloniae (2013)

Gabriela Lorenc–Plucińska

The mechanism of sucrose transport and the influence of various concentrations of sulfite on its activity was studied in the mesophyll vacuoles isolated from cottonwood (Populus deltoides Bartr. ex Marsh) leaves. Kinetic analysis of [14C] sucrose uptake by isolated vacuoles revealed the presence of a saturable component with Km for sucrose uptake = 23 mM and Vmax = 21.2 nmol sucrose 10-6 vacuoles min-1. The saturable component was inhibited by SH-blocker, p-chloromercuribenzenesulfonic acid (PCMBS) and was substrate specific. The transport of sucrose was not changed by ATP with or without Mg2+, by the ionophore, valinomycin and by protonophores, carbonylcyanide m-chlorophenyl hydrazone (CCCP) or dinitrophenol (DNP). Sulfite at concentrations of 0.01-2.5 mM inhibited the uptake of [14C] sucrose by isolated vacuoles. Inhibition was of the noncompetitive type with K; = 2.48 mM of sulfite (22oC).

Valinomycin

10.5586/asbp.1996.044

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The effect of sulphite on the regulation of photosynthetic sucrose synthesis in poplar leaves

Journal of Experimental Botany (1998)

Gabriela Lorenc–Plucińska

Sulphite at concentrations from 0.05 to 5.0 mM was supplied to illuminated, detached poplar (Populus deltoides Bartr. ex Marsh) leaves via the transpiration stream. The rate of CO2 fixation and partitioning of newly fixed carbon between sucrose and starch were measured and compared with the contents of selected phosphorylated intermediates, the contents of fructose-2,6-bisphosphate (Fru2,6BP) and the activation of sucrose-phosphate synthase (SPS). Supplying leaves with ≤0.5 mM sulphite led to an increase in the sucrose/starch partitioning ratio without altering the rate of 14CO2 fixation. The increase in sucrose synthesis compared to starch synthesis was accompanied by relatively small changes of 3-phosphoglyceric acid (PGA), fructose-1,6-bisphosphate (Fru1,6BP), hexose phosphates (hexose-P), uridine 5′-diphospho-glucose (UDPGlc), an accumulation of triose phosphates (triose-P), an activation of SPS, and decreased Fru2,6BP contents. Supplying leaves with ≥1.0 mM sulphite decreased 14CO2 assimilation and increased partitioning of fixed carbon into starch. A selective inhibition of sucrose synthesis was accompanied by an accumulation of triose-P, Fru1,6BP, hexose-P, and a decrease of PGA contents. There was also a large increase of Fru2,6BP contents and a decline in the activation of SPS. It could be argued that sulphite affects the allocation of photosynthetic carbon to sucrose and that sulphite can inhibit photosynthesis via a selective inhibition of sucrose synthesis.

Carbon fixation

Hexose

Fructose 2,6-bisphosphate

Sugar phosphates

10.1093/jxb/49.319.213

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Citations (6)

Effects of HF on photosynthesis, photorespiration and dark respiration of Scotch pine

Gabriela Lorenc–Plucińska Jacek Oleksyn

Photorespiration

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