Olga Kofanova

Luxembourg Institute of Health

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Co-Authors

Fay Betsou

Institut Pasteur

N. G. Zemlyanskikh

Institute for Problems of Cryobiology and Cryomedicine

Camille Bellora

University of Luxembourg

Johanna Trouet

University of Luxembourg

Achilleas Pexaras

Luxembourg Institute of Health

Ingolf Bernhardt

Saarland University

Rocío Aguilar‐Quesada

Andalusian School of Public Health

Sónia Frasquilho

Central Bank of Luxembourg

Mars Stone

Vitalant

Pierre Lescuyer

University Hospital of Geneva

Cooperative Institutions

Luxembourg Institute of Health

123

University of Luxembourg

116

Centre Hospitalier de Luxembourg

Laboratoire National de Santé

National Library of Luxembourg

Central Bank of Luxembourg

Maastricht University Medical Centre

Inserm

University of Gothenburg

Friedrich-Alexander-Universität Erlangen-Nürnberg

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Lithostatine Concentration Is not Useful for Assessing the Preanalytical Variations in Biobanked Urine Samples

Biopreservation and Biobanking (2013)

Olga Kofanova Fay Betsou

Proteomic research requires high-quality, standardized samples. Quality control (QC) biomarkers, which are sensitive to the collection, processing or storage conditions, would be useful tools to identify compromised samples. This study evaluates the usefulness of renal lithostatine as a QC tool for urine sample processing in daily biobank work. Four factors (pre-analytical variations) were examined for their effect on renal lithostatine as measured by ELISA: time from sample collection to centrifugation, number of specimen freeze-thaw cycles, specimen preservation with protease inhibitors, and the inclusion or exclusion of urinary sediment.

Sample (material)

Sample Preparation

10.1089/bio.2013.0031

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Citations (1)

Induced pluripotent stem cells biobank perspective: from unresolved barriers to stable production

Problems of Cryobiology and Cryomedicine (2022)

Lucie Remark Olga Kofanova

10.15407/cryo32.04.302

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Citations (0)

Learning from cryobiology to improve biopreservation quality in a biobank

Problems of Cryobiology and Cryomedicine (2022)

Achilleas Pexaras Olga Kofanova

Cryobiology

Biopreservation

10.15407/cryo32.04.301a

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Modifications of Membrane-Cytoskeleton Proteins on the Human Erythrocyte Cryopreservarion in the Glycerol Presence

Problems of Cryobiology and Cryomedicine (2008)

Olga Kofanova N. G. Zemlyanskikh

The purpose of the work was the investigation of membrane-cytoskeleton complex modification in erythrocytes exposed in glycerol solution and cryopreserved under its protection in media with different ionic strength. It was shown that glycerol influenced weakly to erythrocyte membrane-cytoskeleton complex structure and the differences in protein spectra of thawed deglycerolized erythrocytes and native ones were not pronounced also.

Band 3

Source

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Ca2+-ATPase of human erythrocytes is modified under glycerol and low temperature effects

Problems of Cryobiology and Cryomedicine (2005)

N. G. Zemlyanskikh Olga Kofanova

Source

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IL8 and IL16 levels indicate serum and plasma quality

Clinical Chemistry and Laboratory Medicine (CCLM) (2018)

Olga Kofanova Estelle Henry Rocío Aguilar‐Quesada Alexandre Bulla Hector Navarro Linares

Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified.Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their "diagnostic performance" in identifying serum or plasma samples with extended pre-centrifugation times.In this study, using R&D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL.These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.

Interleukin 8

10.1515/cclm-2017-1047

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Citations (19)

DNA Fingerprinting: A Quality Control Case Study for Human Biospecimen Authentication

Biopreservation and Biobanking (2014)

Olga Kofanova William Mathieson Gerry Thomas Fay Betsou

This case study illustrates the usefulness of the DNA fingerprinting method in biobank quality control (QC) procedures and emphasizes the need for detailed and accurate record keeping during processing of biological samples. It also underlines the value of independent third-party assessment to identify points at which errors are most likely to have occurred when unexpected results are obtained from biospecimens.

DNA profiling

Quality Control

10.1089/bio.2013.0063

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Citations (13)

Standardization of the preanalytical phase of DNA extraction from fixed tissue for next-generation sequencing analyses

New Biotechnology (2019)

Olga Kofanova Camille Bellora Sónia Frasquilho Laurent Antunes Gaël Hamot

Next-generation sequencing (NGS) analyses on DNA derived from archived Formalin-Fixed Paraffin-Embedded (FFPE) clinical material can provide a powerful tool in oncology research and clinical diagnostics. Although several studies have established that NGS can be performed using DNA from FFPE tissue, the accuracy and reproducibility of such analyses, as well as their robustness to the biomolecular quality of the samples used, remains a matter of debate. Excellent reviews have recently been published, providing evidence-based best practices for FFPE DNA extraction. Alternative fixatives exist, although their implementation in clinical practice is difficult. In this article, we present (i) a review of fixed tissue DNA preanalytics with a special focus on DNA extraction and fixed tissue sample qualification and (ii) results from comparisons between different methods of DNA extraction from tissue samples that have been fixed or stabilized by different methods, in terms of NGS metrics and different DNA quality metrics.

Tissue sample

Clinical Practice

10.1016/j.nbt.2019.07.005

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Citations (24)

Biospecimen Qualification in a Clinical Biobank of Urological Diseases

Biopreservation and Biobanking (2023)

Olga Kofanova Sangita Paul Achilleas Pexaras Camille Bellora Ala Petersons

Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability of the biomarkers to be analyzed and can seriously impact analytic outcomes. Sample quality stratification assays and tools can be utilized by biorepositories to minimize bias resulting from samples' inconsistent quality. In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.

Biomarker Discovery

Biorepository

10.1089/bio.2022.0190

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Citations (0)

Changes in the intracellular Ca2+ content in human red blood cells in the presence of glycerol

Bioelectrochemistry (2008)

Olga Kofanova N. G. Zemlyanskikh Lyubomira Ivanova Ingolf Bernhardt

Vanadate

HEPES

Intracellular pH

10.1016/j.bioelechem.2008.04.025

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Citations (13)