Objective To determine the neurotrophic effect of rh-HBNF on regeneration of injured peripheral nerve in rats. Methods A sciatic injury model was induced in SD rats. Sixty female SD rats with induced sciatic injury were randomly divided into HBNF-treated group and control group. In the following 3 days 10μl (0.1mg) rh-HBNF and 0.9% Nacl were injected (q.d) to the injured sciatic nerve of both groups respectively. At the 1st, 2nd, 3rd, 4th weeks, six rats from both groups were sacrificed respectively, the pathological exams were performed. The sciatic function indexes(SFI) of remaining 6 rats in each group were examined till week 10. Results The SFIs in HBNF-treated group were higher than those in control group (P0.05) at the 2nd and 3rd weeks; the results of pinch test were also higher in HBNF-treated group than those in control group at the 2nd and 3rd weeks (P0.05). Histological findings showed that the cross-sectional areas of distal nerve and the proximal nerve, the nerve pass rate and the myelinated axon diameter in HBNF-treated group were all greater than those in control group(P0.05 or P0.01). Conclusion rh-HBNF may promote the recovery of the injured peripheral neural system.
A matched-pairs animal study.To confirm the accuracy of magnetic resonance imaging (MRI) as a means of evaluating edema and fat degeneration of the multifidus muscle by comparing measurements made using MRI with those made using histological examination.MRI is considered a reliable means of evaluating multifidus muscle edema and fat degeneration. However, it is not clear whether its results are always consistent with histological findings.Models of different degrees of multifidus injury were created at the L2-L3, L3-L4, and L4-L5 disc levels in 56 New Zealand white rabbits. These were divided into 4 groups and subjected to different processes: sham surgery, dissection and stripping of the multifidus, crushing of the muscle lasting 1 hour, and crushing of the muscle lasting 2 hours. Two rabbits per group were examined at each of the indicated points in time. Multifidus edema was assessed using fat-suppressed T2 signal intensity ratio of gross multifidus to psoas (T2R) on MRI bilaterally, wet weight and wet:dry weight ratio on the left side (edema-left), and visual edema score on the right side (edema-right). Muscle fat degeneration was detected bilaterally using the T1 signal intensity ratio of gross multifidus to psoas with MRI (T1R) and visual fat degeneration score (fat score) with histology.Pearson correlation coefficient analyses showed significant correlations (P < 0.001) between left T2R and edema-left (r = 0.927), right T2R and edema-right (r = 0.868), and T1R and fat score (r = 0.804). A paired t test demonstrated no significant differences between MRI measurements and histological changes (P = 0.999, 1.000, and 0.998). Bland-Altman plots also depicted good agreement between MRI measurements and histological changes (limits of agreement: left multifidus edema, ± 0.75; right multifidus edema, ± 1.01; fat degeneration, ± 1.23).The MRI technique is an accuracy means of evaluating multifidus muscle injury and atrophy.
To determine the enhancement effects of caffeine on chemotherapy of transplanted osteosarcoma in Fischer 344/N rats.Osteosarcoma-bearing Fischer 344/N rats were treated with cisplatin 2.5 mg/kg (Group DDP), caffeine 90 mg/kg x 2 d (Group caffeine), and cisplatin 2.5 mg/kg plus caffeine 90 mg/kg x 2 d (Group DDP+caffeine), and the control group was treated with normal saline in the same volume. All drugs were given by intra-peritoneum injection with micro-pump, in the rate of 0.5 ml/h. The tumor volume was measured and evaluated. The tumors were stained in TUNEL, and PCNA was detected with immunohistochemistry. The tumor growth inhibition rate, PCNA index and apoptosis index were calculated, and the survival time were recorded.The tumor inhibition rate was -0.5219 +/-0.1429 in control group, 0.0362 +/-0.0957 in Group DDP, -0.4193 +/-0.1345 in Group caffeine, and 0.3646 +/-0.1313 in Group DDP+caffeine (P <0.01). PCNA index was 0.4587 +/-0.1312 in control group, 0.1847+/-0.0535 in Group DDP, 0.4381 +/-0.0706 in Group caffeine, and 0.0314 +/-0.0231 in Group DDP+caffeine (P <0.01). Apoptosis index was 0.0008 +/-0.0005 in control group, 0.0077 +/-0.0060 in Group DDP, 0.0011 +/-0.0003 in Group caffeine, and 0.0295 +/-0.0069 in Group DDP+caffeine (P <0.01). And the survival time was (33.63 +/-4.63)d in control group, (52.13 +/-11.74)d in Group DDP, (35.63 +/-5.15)d in Group caffeine, and (55.13 +/-16.23)d in Group DDP+caffeine (P <0.01).Caffeine could enhance the anti-tumor effect of cisplatin in rat osteosarcoma.
Objective To investigate the efficacy of combined or single drugs of methylprednisolone (MP) and nimodipine in treatment of early spinal cord injury in rats.Methods Eighty rats were completely paralyzed by aneurysm clip method and randomized into four groups,in which intravenously MP,nimodipine,MP combined with nimodipine and no treatment were administrated respectively.Tarlov scale and inclined plane test were performed at the end of 1,2,3 and 4 weeks after injury,and the histopathologic and immunohistochemical examinations,image analysis,CSEP and SCEP examinations were also performed. Results The Tarlov scale and inclined plane test showed significant differences between three treatment groups and control group and no significant differences among three treatment groups.The results of image analysis showed significant differences between treatment groups and control group,and the combined treatment group showed significant difference with single MP and nimodipine group.The CSEP remained no recovery and the rate of SCEP recovery showed significant differences between treatment groups and control group, meanwhile, no significant difference among treatment groups.Immunohistochemical results showed negative 5-HT fibers in ventral horn of control group but that was positive in treatment groups.Conclusion MP and nimodipine were effective in treatment of early spinal cord injury and both showed neuron protective effects, but there was no significant difference between combined therapy and single-drug therapy.
A reliability study was conducted.To estimate the intra- and intermeasurement errors in the measurements of functional cross-sectional area (FCSA), density, and T2 signal intensity of paraspinal muscles using computed tomography (CT) scan and magnetic resonance imaging (MRI).CT scan and MRI had been used widely to measure the cross-sectional area and degeneration of the back muscles in spine and muscle research. But there is still no systemic study to analyze the reliability of these measurements.This study measured the FCSA and fatty infiltration (density on CT scan and T2 signal intensity on MRI) of the paraspinal muscles at L3-L4, L4-L5, and L5-S1 in 29 patients with chronic low back pain. Two experienced musculoskeletal radiologists and one superior spine surgeon traced the region of interest twice within 3 weeks for measurement of the intra- and interobserver reliability.The intraclass correlation coefficients (ICCs) of the intra-reliability ranged from fair to excellent for FCSA, and good to excellent for fatty infiltration. The ICCs of the inter-reliability ranged from fair to excellent for FCSA, and good to excellent for fatty infiltration. There were no significant differences between CT scan and MRI in reliability results, except in the relative standard error of fatty infiltration measurement. The ICCs of the FCSA measurement between CT scan and MRI ranged from poor to good.The reliabilities of the CT scan and MRI for measuring the FCSA and fatty infiltration of the atrophied lumbar paraspinal muscles were acceptable. It was reliable for using uniform one image method for a single paraspinal muscle evaluation study. And the authors preferred to advise the MRI other than CT scan for paraspinal muscles measurements of FCSA and fatty infiltration.
To observe the cytotoxic effect of thermo-chemotherapy with cisplatin on osteosarcoma OS-732 cell line and to explore its mechanism.The osteosarcoma OS-732 cell line was treated with different temperature, cisplatin alone and 43 degrees C +cisplatin for 1 h, respectively and the in vitro cytotoxic effect was observed with MTT assay. The cell cycle and the apoptotic rate were analyzed with flow cytometry (FCM); the cellular apoptosis was observed also with electron microscope.The cytotoxicity index increase markedly as the temperature elevated or the concentration of cisplatin increased. While treated with 43 degrees C hyperthermia and cisplatin simultaneously, the cytotoxicity index increased to 72.37%;the cell cycle of the treated OS-732 cells line was changed with marked increase in S phase and decreasing in G(2)/M phase. The apoptotic rate increased markedly with the highest of 56.47%. Electron microscope showed the characteristic apoptotic alterations.Hyperthermia with cisplatin enhance cytotoxicity on osteosarcoma OS-732 cell line and the induced cell apoptosis may be one of the mechanisms of enhanced cytotoxicity by thermo-chemotherapy.
Objective To analyze and compare clinical application of two types of clavicular hook plates. Methods From September 2000 to October 2003, 33 cases with acromioclavicular joint dislocation (Tossy Ⅲ) or distal clavicle fractures (Neer Ⅱ) were treated with insertion type (AO/ASIF) or knuckle-protruded type (WALDEMAR LINK) clavicular hook plates. The former one was used in 18 cases and the latter one in 15. Results In insertion type group, the average operation time was 28 minutes, with average incision length for 7.6 cm. All the cases got good reduction and fixation without any subluxation, except for one case suffering from impingement of the shoulder joint. In knuckle-protruded type group, the average operation time was 40 minutes, with an average incision length for 9.8 cm. After operation, one case lost hooking-up, one had born shoulder joint impingement, while another two suffered from skin pain at acromion caused by hook tip for a long time. Except for one case losing hooking-up, all cases of both groups achieved good recovery of function of shoulder joint and won satisfactory reduction without any redislocation after the implants being taken out. Conclusion Because of the advantages like convenient manipulation, short operation time, minor incisions and less potential complications, the insertion type clavicular hook plate is more suitable for cases with Tossy Ⅲ or Neer Ⅱ fractures.
Objective To discuss the effect of the sustained releasing agent FK506 on the ultrastructure of the regenerative nerve fibers.Methods The new fusiform-shaped double channel nerve conduit was used for bridge the sciatic nerve defects for 10 mm in 32 Sprague-Dawley rats.The rats were divided into Group A(100μl chitin for both channels)and Group B(chitin plus FK506 for B1 and chitin plus normal saline for B2)according to different addition of the drugs.At 8 and 12 week after operation,the middle line of the regenerated nerve was observed under the transmission electron microscope.In the meantime,an analysis was done on the area of the regenerated nerve fibers(myelinated and unmyelinated),diameter and myelin thickness of the myelinated fiber axon at 8 and 16 months.Results There was not significant difference in aspects of type and number of the regenerative nerve fibers between two channels in Group A.However,the myelinated and unmyelinated fibers of the regenerative nerves were increased in Group B1,with larger area than Group B2(P < 0.05).Conclusion FK506 can significantly promote the regeneration of both the myelinated and unmyelinated fibers.
Key words:
Tacrolimus binding protenis; Peripheral nerve regeneration; Ultrastructure; Counting research
Objective:To provide experimental proofs for cisplatin combined with carboplatin in therapy of osteosarcoma through comparing the cytotoxity of half dose cisplatin combined with half dose carboplatin,cisplatin and arboplatin in osteosarcoma cell line(OS-732).Methods:OS-732 cells were treated by IC 50 of cisplatin,IC 50 of carboplatin,or 1/2 IC 50 cisplatin combined with 1/2 IC 50 carboplatin,and cultured 48h or 72h.Then their viability was determined by the colorimetric MTT assay.Results:When cells were cultured for 48hrs,cytotoxicities of group IC 50 cisplatin,IC 50 carboplatin and 1/2 IC 50 cisplatin+1/2 IC 50 carboplatin for OS-732 cells had no significant difference( P 0 05);when cells were cultured for 72 hrs,cytotoxicities of group IC 50 carboplatin and group 1/2 IC 50 cisplatin+1/2 IC 50 carboplatin for OS-732 cells were higher than that of group IC 50 cisplatin( P 0 05)Conclusion:Cisplatin combined with carboplatin as a “single agent” appears to be as active as cisplatin or carboplatin alone in