AbstractAbstractThe test of induction of lambda-phage formation in lysogenic Escherichia coli K-12(λ) was used in a study of some medicinal plants from Iran. In this study, 19 plants from North-eastern of Iran (Turkmen Sahra) were chosen based on ethnobotanical information and screened for biologic activity. 100μg of dried extract dissolved in DMSO were spotted to the surface of the plates including E.coli ,strain K12 (λ). Mitomycin C is used as positive control to check the efficiency of the experiment. The active extract cause the prophage to be released from the host genome and the phage reverts to the lytic mode and lyses cells. Production of plaque shows this effect. Extract from roots of Ferula szowitsiana, Stachys turcomanicaTrautv, Satureja muticaFisch, Echium italicum, Hymenocrater platystegius, Glycyrrhiza glabrashowed positive effect. One of the 6 effective plants, Ferula szowitsiana, was chosen for fractionation and further studies. The induction test may provide a useful screen for the detection of potential DNA-reactive agents.Key Words: Lambda-phageFerula szowitsianaDNA-reactive Agents
New bio-based thermosetting resin was synthesized from palm oil. In this study the epoxy groups presented on the epoxidized palm oil (EPO) were first acrylated and then further maleinized. The acrylation reaction was done by introducing acrylic acid into epoxy group of the epoxidized palm oil. Hydroquinone and triethylamine were used as inhibitor and catalyst, respectively. This reaction was confirmed by Fourier Transform Infrared Spectroscopy (FTIR). To render acid groups on the resulting monomer, the acrylated epoxidized palm oil (AEPO) was further reacted with maleic anhydride. The resulting maleinated acrylated epoxidized palm oil (MAEPO) was characterized by FTIR and Nuclear Magnetic Resonance Spectroscopy ( 1 H NMR).
The separation of fatty acids derivatised with Nile Blue (NB) by CE with detection using a red light-emitting diode (LED) was examined. NB was selected as the derivatisation agent due to its high molar absorption coefficient of 76,000 M(-1) cm(-1) at 633 nm, making it well suited for sensitive absorbance detection using a red 635 nm LED. NB-labelled fatty acids were separated by both MEKC using SDS micelles, i-PrOH and n-BuOH and by NACE in a number of solvents including MeOH, EtOH and ACN. The sensitivity of NACE was superior to MEKC, with detection limits of 5x10(-7)-7x10(-7) M obtained for each acid, approximately 20 times lower than the MEKC method. The NACE detection limits are approximately 100 times lower than previous reports on the separation of fatty acids by CE using indirect absorbance detection, ten times lower than using indirect fluorescence detection and are inferior only to those obtained using precapillary derivatisation and direct fluorescence detection. The efficiency of the NACE method was also superior to MEKC and allowed the separation of unsaturated fatty acids to be examined, although it was not possible to baseline-resolve linoleic (C18:2) and linolenic (C18:3) acids in a reasonable time. The method was used to analyse the fatty acid profile of two edible oils, namely sunflower and sesame oils, after alkali hydrolysis, where it was possible to identify both the saturated and unsaturated fatty acids in each sample.
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