The phenomenon of rock movement in mining areas has always been a difficult problem in mining engineering, especially under complicated geological conditions. Although the backfilling method mitigates the destruction of the surrounding rock, deformation can still exist in the mining area. In order to ensure the safety of under-sea mining, it is necessary to study the rock movement laws and the mechanisms. This paper focuses on a settlement analysis of the monitoring data of the No. 55 prospecting profile. By analyzing the shape of the settlement curves, the spatial distribution characteristics of settlements of different mining sublevels are summarized. Additionally, the fractal characteristics of the settlement rate under different space–time conditions are studied. We also discuss the relationship between the fractal phenomenon and the self-organized criticality (SOC) theory. The findings are of great theoretical value for the further study of mining settlements to better understand the physical mechanisms of internal movement and rock mass failures through the fractal law of the settlement. Furthermore, elucidating the rock movement law is an urgent task for the safety of seabed mining.
Radical innovation is an important opportunity for the technology backwardness enterprises to catch up with and surpass the leader.Through the exploratory case study, the study discovers the mechanism of how the Chinese wind power manufacturing enterprises realize the radical innovation by embedding in global industrial chain.The study finds that: 1) With the constant improvement of the embedding in global industrial chain level, the ability of integrating global industrial chain resources will be increased gradually; 2) The integration of global industrial chain resources includes integrating resource from suppliers technology and the market worldwide;3) The ability of integrating global industrial chain resources has a positive effect on radical innovation performance.
Abstract Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 10 8 CFU/ml, a medium-dose group of 10 9 CFU/ml, and a high-dose group of 10 10 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.
The ASGR-mediated endocytosis has been successfully applied to the hepatocyte-targeted delivery of therapeutic oligonucleotides via glycoconjugates. However, few studies have explored the conjugated small molecules due to the challenge of cleaving suitable linkers for the release of active small molecules, which is especially different from GalNAc-ONs cleaved by the deoxyribonuclease II in the lysosome. In this study, GalNAc-MMAE conjugates linked by CTSB-cleavable linkers were designed and synthesized. A comprehensive approach revealed that the conjugates were endocytosed by ASGR and subsequently hydrolyzed by CTSB, releasing MMAE. The optimized conjugate with a succinic acid monoamide as the fragment of the linker demonstrated favorable plasma stability, excellent biodistribution, and significant antitumor activities in vivo with weight gain at the effective dose in an orthotopic hepatocellular carcinoma mouse model. This research provides a strategy for developing anti-HCC therapeutic agents using GalNAc drug conjugates with CTSB-cleavable linkers to release active small molecules.
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