Sogand Vahidi

Kermanshah University of Medical Sciences

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Ali Akbar Samadani

Guilan University of Medical Sciences

Seyedeh Elham Norollahi

Semnan University of Medical Sciences

Arefeh Zabeti Touchaei

Islamic Azad University, Lahijan Branch

Kosar Babaei

Non-Communicable Diseases Research Center

Ebrahim Mirzajani

Guilan University of Medical Sciences

Shima Shams

Guilan University of Medical Sciences

Saba Sorayyayi

Ardabil University of Medical Sciences

Armin Soleymanpour

Guilan University of Medical Sciences

Morteza Rahbar Taramsari

Guilan University of Medical Sciences

Arman Keymoradzdeh

Shahid Beheshti University of Medical Sciences

Cooperative Institutions

Guilan University of Medical Sciences

Razi Hospital

Ardabil University of Medical Sciences

University of Guilan

Semnan University of Medical Sciences

University of Neyshabur

Shahid Beheshti University of Medical Sciences

Non-Communicable Diseases Research Center

Islamic Azad University Tonekabon

Iran University of Medical Sciences

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Immune landscape in APC and TP53 related tumor microenvironment in colon adenocarcinoma: A bioinformatic analysis

European Journal of Microbiology and Immunology (2024)

Arefeh Zabeti Touchaei Sogand Vahidi Ali Akbar Samadani

Abstract Introduction APC and TP53 are the two most regularly mutated genes in colon adenocarcinoma (COAD), especially in progressive malignancies and antitumoral immune response. The current bioinformatics analysis investigates the APC and TP53 gene expression profile in colon adenocarcinoma as a prognostic characteristic for survival, particularly concentrating on the correlated immune microenvironment. Methods Clinical and genetic data of colon cancer and normal tissue samples were obtained from The Cancer Genome Atlas (TCGA)-COAD and Genotype-Tissue Expression (GTEx) online databases, respectively. The genetic differential expressions were analyzed in both groups via the one-way ANOVA test. Kaplan–Meier survival curves were applied to estimate the overall survival (OS). P < 0.05 was fixed as statistically significant. On Tumor Immune Estimation Resource and Gene Expression Profiling Interactive Analysis databases, the linkage between immune cell recruitment and APC and TP53 status was assessed through Spearman's correlation analysis. Results APC and TP53 were found mutated in 66.74% and 85.71% of the 454 and 7 TCGA-COAD patients in colon and rectosigmoid junction primary sites, respectively with a higher log2-transcriptome per million reads compared to the GTEx group (318 samples in sigmoid and 368 samples in transverse). Survival curves revealed a worse significant OS for the high-APC and TP53 profile colon. Spearman's analysis of immune cells demonstrated a strong positive correlation between the APC status and infiltration of T cell CD4+, T cell CD8+, NK cell, and macrophages and also a positive correlation between status and infiltration of T cell CD4+, T cell CD8+. Conclusions APC and TP53 gene mutations prevail in colon cancer and are extremely associated with poor prognosis and shortest survival. The infiltrating T cell CD4+, T cell CD8+, NK cell, and macrophages populate the colon microenvironment and regulate the mechanisms of tumor advancement, immune evasion, and sensitivity to standard chemotherapy. More comprehensive research is needed to demonstrate these results and turn them into new therapeutic outlooks.

10.1556/1886.2024.00015

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DNA Methylation Profiling of MYC, SMAD2/3 and DNMT3A in Colorectal Cancer

Oman Medical Journal (2021)

Seyedeh Elham Norollahi Maryam Foumani Maryam Khoshbakht Pishkhan Afshin Shafaghi Majid Alipour

Epigenetic modifications, particularly DNA methylation, is commonplace and a remarkable factor in carcinogenesis transformation. Conspicuously, previous findings have presented a cluster of irregular promoter methylation alterations related with silencing of tumor suppressor genes, little is accepted regarding their sequential DNA methylation (hypo and hyper) modifications during the cancer progression. In this way, fluctuations of DNA methylation of many genes, especially MYC, SMAD2/3, and DNMT3A, have an impressive central key role in many different cancers, including colorectal cancer (CRC). CRC is distinguished by DNA methylation, which is related to tumorigenesis and also genomic instability. Importantly, molecular heterogeneity between multiple adenomas in different patients with CRC may show diverse developmental phenotypes for these kinds of tumors. Conclusively, studying factors that are involved in CRC carcinogenesis, especially the alterations in epigenetic elements, such as DNA methylation besides RNA remodeling, and histone modification, acetylation and phosphorylation, can be influential to find new therapeutic and diagnostic biomarkers in this type of malignancy. In this account, we discuss and address the potential significant methylated modifications of these genes and their importance during the development of CRC carcinogenesis.

Cancer Epigenetics

Epigenomics

10.5001/omj.2020.93

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Novel Biomarkers of microRNAs in Gastric Cancer: An Overview from Diagnosis to Treatment

MicroRNA (2022)

Ebrahim Mirzajani Sogand Vahidi Seyedeh Elham Norollahi Ali Akbar Samadani

Abstract: Gastric cancer (GC) is the fourth most frequent disease in the world and the second cause of cancer-related death. In this way, over 80% of diagnoses are made in the middle to advanced de-grees of the disease, underscoring the requirement for innovative biomarkers that can be identified quickly. Meaningly, biomarkers that can complement endoscopic diagnosis and be used to detect patients with a high risk of GC are desperately needed. These biomarkers will allow for the accurate prediction of therapy response and prognosis in GC patients, as well as the development of an opti-mal treatment strategy for each individual. Conspicuously, microRNAs (miRNAs) and small non-coding RNA regulate the expression of target mRNA, thereby modifying critical biological mecha-nisms. According to the data, abnormally miRNAs expression in GC is linked to tumor growth, car-cinogenesis, aggression, and distant metastasis. Importantly, miRNA expression patterns and next-generation sequencing (NGS) can also be applied to analyze different kinds of tissues and cancers. Given the high death rates and poor prognosis of GC, and the absence of a clinical diagnostic factor that is adequately sensitive to GC, research on novel sensitive and specific markers for GC diagno-sis is critical. In this review, we examine the latest research findings that suggest the feasibility and clinical utility of miRNAs in GC.

Diagnostic biomarker

10.2174/2211536611666220322160242

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The fluctuation of APC gene in WNT signaling with adenine deletion of adenomatous polyposis coli, is associated in colorectal cancer

Journal of Coloproctology (2019)

Seyedeh Elham Norollahi Seyed Mohammad Taghi Hamidian Zeynab Khazaee Kohpar Rezvan Azadi Pooya Rostami

Abstract Colorectal cancer is one of the most important malignancies in the classification of gastrointestinal cancers. One of the predisposing factors at molecular level for this cancer is via WNT signaling which is associated with the vast numbers of different genes. Thus, in this study, we aimed to investigate whether Adenomatous Polyposis Coli gene (APC) mutation of rs41115in two locations such as 132.002 and 131.989 acts as a trigger or cause of colorectal cancer. Relatively, 30 blood samples of colorectal cancer patients and 30 normal blood samples as control group after colonoscopy and also confirmation of pathology report at Rohani Hospital in Babol (Iran) were investigated. The primers were designed in order to be included the rs41115 to identify the particular polymorphisms of gene. The polymerase chain reaction (PCR direct sequencing method) was used. Conclusively, deletion of adenine in two specific locations such as 131.989 and 132.002 has been identified, but there was no relationship between rs41115 polymorphisms located in adenomatous polyposis coli gene and colorectal cancer.

Adenomatous polyposis coli

10.1016/j.jcol.2019.11.487

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MUTYH and KLF6 gene expression fluctuations in tumor tissue and tumor margins tissues of colorectal cancer

Journal of the Egyptian National Cancer Institute (2022)

Hoora Naebi Ahmadreza Bandegi Fereshteh Talebinasab Pirouz Samidoust Seyedeh Elham Norollahi

Colorectal cancer (CRC) is one of the most important cancers in the world, and its prevalence varies depending on the geographical area. Genetically, tumor regeneration in CRC as a multi-step process involves activating mutations in protocogenes and losing the function of tumor suppressor genes as well as DNA repair and recovery genes. Occur in this way, our goal was to investigate the expression of KLF6 genes as a tumor suppressor and MUTYH involved in the DNA repair process in colorectal cancer.This research was conducted during the years 2019-2018 in Razi Hospital, Rasht. The subjects included 30 tumoral and 30 non-tumoral tissues of colorectal cancer and 20 healthy controls. The real-time PCR method was used to investigate the gene expression. For data analysis by SPSS, parametric statistical tests ANOVA and T test and regression analysis were used and p value values less than 0.05 were considered significant.The expression of KLF6 gene in tumoral tissues showed a significant decrease compared to non-tumoral tissues (P = 0.04). Also, the expression of MUTYH gene in tumor tissue showed a significant decrease compared to non-tumoral (P = 0.02) and this decrease in MUTYH gene expression had a significant relationship with increasing tumor stage (P = 0.01).These findings suggest that decreased expression of KLF6 and MUTYH genes in the study population has a significant relationship with colorectal cancer and can be considered as tumor marker in diagnostic purpose.

MUTYH

10.1186/s43046-022-00158-9

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Effect of miRNA-1266-5p repression on the increasing cell survival and alterations of the cell cycle in AGS cell line

DOAJ (DOAJ: Directory of Open Access Journals) (2018)

Saba Sorayyayi Sogand Vahidi Mohammad Mohammadzadeh-Vardin Sayyed Saied Hosseini-Asl

Line (geometry)

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Performance of KLF4 and Wnt1 genes expression fluctuations in tumoral and margin tissues of colorectal cancer in an Iranian population

Gene Reports (2021)

Fereshteh Talebinasab Ahmadreza Bandegi Hoora Naebi Hossein Hemmati Pirouz Samidoust

KLF4

10.1016/j.genrep.2021.101465

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CAR T-cells profiling in carcinogenesis and tumorigenesis: An overview of CAR T-cells cancer therapy

International Immunopharmacology (2020)

Ali Akbar Samadani Arman Keymoradzdeh Shima Shams Armin Soleymanpour Ali Rashidy‐Pour

Cancer Immunotherapy

Cell therapy

Cancer Therapy

10.1016/j.intimp.2020.107201

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Citations (22)

Therapeutic combinations of exosomes alongside cancer stem cells (CSCs) and of CSC-derived exosomes (CSCEXs) in cancer therapy

Cancer Cell International (2024)

Arefeh Zabeti Touchaei Seyedeh Elham Norollahi Ali Najafizadeh Kosar Babaei Elahe Bakhshalipour

Cancer Therapy

10.1186/s12935-024-03514-y

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Citations (1)

The Effect of Human Telomerase Reverse Transcriptase Repression on the Increasing Cell Viability and Alterations of Cell Cycle in Gastric Cancer Cell Line

GOVARESH (2018)

Sogand Vahidi Saba Sorayayi Mohammad Mohammadzadeh-Vardin S.S. Hosseini-Asl

Background: Telomerase is a ribonucleoproteins enzyme responsible for the maintenance of telomere length. Human telomerase reverse transcriptase (hTERT) is a major component of the catalytic subunit of telomerase enzymes and is expressed in cells that have telomerase activity but is not expressed in normal somatic cells. Based on the specific expression of hTERT in most cancer cells, it can be considered as a factor in the distinction between cancer cells and normal cells. It seems that inhibiting the expression of hTERT has been presented as a therapeutic approach in inhibiting the activity of telomerase. One of the special tools for inhibiting genes is the use of small interfering RNA (siRNAs). The purpose of this study was to investigate the effect of hTERT gene on the cell viability and cell cycle in gastric cancer cells. Materials and Methods: In this study, human cancer cells, adenocarcinoma gastric cell line (AGS) were cultured in RPMI 1640 medium (Roswell Park Memorial Institute) containing 10% FBS (Fetal Bovine Serum) and 1% penicillin/streptomycin antibiotics. The suppression of the hTERT gene was accomplished by FlexiTube siRNA. The repression effect of hTERT gene was investigated on cell viability by MTT assay [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] with ELISA (Enzyme Linked Immunosorbent Assay) reader at 570 nm, and cell cycle performed by flow cytometry and DAPI (4', 6-diamidino-2-phenylindole) staining. Results: The effect of hTERT siRNA on the cell viability by MTT assay showed time dependent cell viability of AGS cell line upon treatment and increasing the exposure time to 48 hours for that concentration decreased AGS cell (p = 0.02). Analysis of flow cytometry also showed increased number of cells in G1 phase and decreased the number of cells in S phase, and induced apoptosis via decreasing the level of hTERT expression. Conclusion: The significant downregulation in hTERT mRNA after 48 hours of hTERT siRNA treatment inhibited the cell viability of AGS cells and cell cycle arrest.

Viability assay

MTT assay

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