OBJECTIVE Through detecting to drug susceptibility results of 12 commonly used antibiotics in Pseudomonas aeruginosa (PAE),we provide the scientific basis for the prevention of infection and reasonable choice of antibiotics. METHODS Twelve antibiotics′ susceptibility tests in vitro were carried out in PAE isolated from lower respiratory tract in Renming Hospital,Wuhan University from Jan 2007 to Dec 2008. RESULTS In the anti-infective drugs,the resistance rate to ceftazidime was the lowest (36.8%),followed by amikacin (39.6%),piperacillin/tazobactam (43.8%),cefepime (47.8%) and cefoperazone/sulbactam (48.5%).The resistant status of PAE was serious and multi-drug resistance existed. CONCLUSIONS The lower respiratory tract infection caused by PAE which possesses single and multiple drug-resistance. We should more think of it and strengthen preventive measures to reduce the rate of infection. Reasonably prudent use of antibiotics is still the best way of delaying its rapid increase of drug-resistance strains.
Objective To investigate the distribution and antimicrobial resistance of microorganism in surgical site infection. Methods 368 patients in-hospital were investigated retrospectively. Results 445 strains were isolated from 368 patients. The dominating microorganisms were Staphylococcus aureus, coagulase negative staphylococcus, Pseudomonas aeruginosa, Escherichia coli, Enterococcus spp, they accont for 89.2%. Most of them were multi drug resistant. Conclusion The antimicrobial resistance is important cause in surgical site infection. To strengthen the surveillance of resistant microorganism could decrease the production of surgical site infection.
Combined with the requirement of real project,the statistic concept of believing degree is introduced into feature extraction and a dynamic algorithm for effective feature extraction is proposed,which is based on accuracy requirement and capable of constringing the feature dimension while reducing leaked or pretend alarm probability.The design detail of a note identification system,which adopts the algorithm,and its test results are introduced.The real applications have verified its effectiveness.
In swine production, weaning is a critical event for porcine weaning-associated disease, such as postweaning stress syndrome, which involves intestinal dysfunction. However, little is known about the molecular mechanisms of intestinal dysfunction in pigs during weaning. To gain new insight into the interaction between weaning stress and intestinal function, 4 pigs at 25 d of age for each of the weaning and the suckling groups for a total of 40 pigs were used to analyze changes in the genomic expression in the intestines of weaned pigs by microarray analysis. Four hundred forty-five genes showed altered expression after weaning treatment (286 upregulated and 159 downregulated) at the cutoff criteria of the fold change ≥1.5 or <0.67 and P < 0.05. Most of these altered genes are cellular process related and regulators that may be involved in biological regulation, developmental processes, and metabolic processes. A keen interest was paid in deciphering expression changes in apoptosis or cell cycle control genes. The altered genomic expression of 8 selected genes related to the cell cycle process was confirmed by quantitative real-time PCR. Of the 8 genes tested, increased (P < 0.05) expression of genes involved in apoptosis (cytochrome c, somatic, and ataxia telangiectasia mutated), pro-inflammatory signals (tumor necrosis factor and NO synthases 2), and a transcription factor (nuclear factor of activated T cells, cytoplasmic, and calcineurin-dependent 2) were detected in weaned pigs compared with suckling pigs, but the expression of cell cycle control-related genes, such as E2F transcription factor 5-like, was lower (P < 0.05) in weaned pigs than suckling pigs. Weaned pigs also showed increased interleukin 8 expression and decreased SMAD family member 4 expression although no significant differences (P > 0.05) were observed when compared with the suckling pigs. These selected genes likely indicate that weaning induced cell cycle arrest, enhanced apoptosis, and inhibited cell proliferation. The results of this study provide a basis for understanding the molecular pathogenesis of weaning treatment.
The effects of silicon (Si) on root development, mineral content, and expression of Si transporter genes in Euphorbia pulcherrima Willd. 'Flame', 'Mable Bell', 'Green Star', 'Pink Bell', and 'Peach Bowl' cultivars were investigated in this study. Stem cuttings in a propagation bench were drenched regularly with a solution containing either 0 (control) or 50 ppm of silicon (Si treatment) from potassium silicate (K2SiO3), with a 25 °C mean air temperature and 80% relative humidity (RH) under 70% shading. The results showed that the 'Flame' treated with Si had a significantly higher survival ratio as compared with that of the control (P ≤ 0.05) and that the Si treatment improved number of roots, length of longest root, fresh root weight, and dry root weight in all cultivars except 'Mable Bell'. Supplementary Si increased the content of magnesium (Mg) and decreased the content of boron (B) and zinc (Zn) in the roots. The content of sulfur (S) in the shoots was increased by supplementary Si. The relative expression of Lsi1 and Lsi2 was higher in 'Peach Bowl', while it was lower in 'Mable Bell' and 'Green Star', which may be caused by the differing accumulation of Si in the shoot. Overall, supplementary Si had beneficial effects during cutting propagation of poinsettia cultivars, although these effects were cultivar-dependent.
The contents of soluble protein and free amino acid,the changes of proteinase activity and types of Osmanthus fragrans‘Liuye Jingui’were determined during flower opening and development stages from linggeng stage,initial flowering stage,full flowering stage,late full flowering stage to wilting stage. The results showed that variation patterns between soluble protein and free amino acid were different. The optimum temperature and pH for protease measure were 37 ℃ and pH 8.0 respectively. The petal proteinase activity which was detected by spectrophotometry and gel-SDS-PAGE electrophoresis increased continuously until late full flowering stage,and then sharply decreased at wilting stage. Three kinds of proteinases,serine protease,cystein protease and metallo protease,were found by using proteinase inhibitors. Serine protease,accounted for about 65%–75% in total,was the most active one among the three proteases to influence petal senescence of sweet osmanthus.
Public safety has always been a hot topic in the world, yet related data are stored in various forms of structures and have a huge amount. The poor characteristics of these huge amounts of heterogeneous data bring great difficulties to its application. For the purpose of applying these data conveniently and efficiently, knowledge graph was adopted. Public safety knowledge graph can help organize public safety data by an organized and properly utilized form, so that it can be used more optimally in public safety related retrieval, question and answer systems, etc. In this paper, unstructured, as well as structured public safety data were used for constructing, with the support of BiLSTM-Attention-CRF model. To be able to reveal this public safety knowledge graph in an understandable way, Neo4j database was used to store public safety knowledge graph.
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