Mitsugu Fukuda

Yao Tokushukai General Hospital

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Nobuko Shimada

Tokyo Metropolitan Institute of Gerontology

Mochihiko Ohashi

Tokyo Metropolitan Institute of Gerontology

Takahiko Taguchi

Tokyo Metropolitan Institute of Gerontology

Narimichi Kimura

Matsuyama University

Naoshi Ishikawa

Tokyo Metropolitan Institute of Gerontology

Hidetaka Miyazaki

Aichi Medical University Hospital

Akihito Ishigami

Tokyo Metropolitan Institute of Gerontology

Yasushi Ishijima

Suzuka University of Medical Science

Kazuhiko Kaji

KOSÉ (Japan)

Naoki Maruyama

Osaka University

Cooperative Institutions

Tokyo Metropolitan Institute of Gerontology

Tokyo Medical and Dental University

The University of Tokyo

Tokyo Metropolitan Geriatric Hospital

Tokyo Metropolitan University

Osaka University

Hiroshima University

Kyoto University

Tokyo University of Science

Shimane University

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Cytogenetic Effects of Argon Laser Irradiation on Chinese Hamster Cells

Radiation Research (1983)

Masaharu Nakajima Mitsugu Fukuda Toshio Kuroki Kazuhiko Atsumi

The cytogenetic effects of argon laser light on V79 Chinese hamster cells were studied. Irradiation with a defocused continuous beam from an argon laser at doses of 100 to 400 J/cm2 had dose-related cytostatic and cytocidal effects. Cell cycle analysis by flow cytofluorometric measurement indicated that argon laser irradiation induced a temporary block at the G2 + M phase. The survival curve after laser irradiation was exponential without an initial shoulder and the D0 value (34% lethal dose) was calculated as 170 J/cm2. A significant increase in sister chromatid exchange was observed after laser irradiation. Laser irradiation also induced chromatid and chromosome aberrations, most of which were breaks and gaps. These chromosomal changes are probably not an artifact of the experimental procedures such as the use of phenol red and antibiotics or spontaneously emitted uv light. No mutation at the Na+/K+-dependent ATPase locus was induced by argon laser irradiation. Although the mechanism by which an argon laser beam induces cytogenetic damage remains to be investigated, the possible involvement of hyperthermia seemed to be excluded.

Chinese hamster

Sister chromatid exchange

10.2307/3576039

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Citations (17)

Regulation of cellular functions by nucleoside diphosphate kinases in mammals.

Journal of Bioenergetics and Biomembranes (2000)

Narimichi Kimura Nobuko Shimada Mitsugu Fukuda Yasushi Ishijima Hidetaka Miyazaki

Neurite

10.1023/a:1005549315846

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Citations (70)

Custom Made Fenestrated Stent Graft Collapse after Thoracic Endovascular Aortic Repair: A Case Report

Case Reports in Clinical Medicine (2023)

Yasuhiko Kobayashi Mitsugu Fukuda Shoji Sakaguchi Yoshihisa Nakao K Nishimine

We present a case of stent graft collapse after performing thoracic endovascular aortic repair with a custom-made fenestrated stent graft. The patient was a 70-year-old woman with an asymptomatic aneurysm of the distal aortic arch, and thoracic endovascular aortic repair was performed. The patient showed a blood pressure difference between the left arm and the right arm on postoperative day (POD) 17 prompting the performance of a chest computed tomography scan which revealed stent graft collapse. She then underwent staged debranching of thoracic endovascular aortic repair. Stent graft collapse is a rare but well-described complication of thoracic endovascular repair. Therefore, patients who undergo such a procedure should be carefully monitored for signs and symptoms, which suggest the possibility of stent collapse.

Aortic repair

Thoracic aortic aneurysm

Aortography

10.4236/crcm.2023.128042

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Citations (0)

Age-dependent changes in DNA polymerase fidelity and proofreading activity during cellular aging

Mechanisms of Ageing and Development (1999)

Mitsugu Fukuda Takahiko Taguchi Mochihiko Ohashi

Klenow fragment

Proofreading

DNA polymerase II

Primer (cosmetics)

10.1016/s0047-6374(99)00034-2

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Citations (9)

Effect of a 3′→5′ exonuclease with a proofreading function on the fidelity of error-prone DNA polymerase α from regenerating liver of aged rats

Mechanisms of Ageing and Development (1998)

Takahiko Taguchi Tosifusa Toda Mitsugu Fukuda Mochihiko Ohashi

Proofreading

DNA polymerase II

DNA clamp

DNA polymerase I

Exonuclease III

Nuclease

Klenow fragment

DNA polymerase mu

Primer (cosmetics)

Nick translation

10.1016/s0047-6374(97)00114-0

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Citations (6)

Erratum to “A biotinylated perfringolysin O derivative: a new probe for detection of cell surface cholesterol”

Biochimica et Biophysica Acta (BBA) - Biomembranes (1998)

Machiko Iwamoto Ikuo Morita Mitsugu Fukuda Sei‐itsu Murota Shinji Ando

Derivative (finance)

10.1016/s0005-2736(97)00253-8

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Preferential Association of Newly Synthesized H3 and H4 Histones with Newly Replicated DNA1

The Journal of Biochemistry (1978)

Tatsuo Senshu Mitsugu Fukuda Mochihiko Ohashi

The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-density centrifugation in a gradient containing Metrizamide and 3-iodo-1,2-propanediol. Histones extracted from heavy and normal chromatin regions of the gradient were fractionated by acid-urea gel electrophoresis, and 3H/14C ratios of individual histones were compared. The results showed highly preferential association of newly synthesized H3 and H4 with newly replicated DNA.

Metrizamide

Micrococcal nuclease

10.1093/oxfordjournals.jbchem.a132213

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Citations (77)

[Effects of the nitric oxide donor SIN-1 on the membrane potential of mouse neuroblastoma-rat glioma hybrid cells].

PubMed (1995)

T Yamasaki Yasuhiko Akiyama Mitsugu Fukuda Yusuke Kimura Kouzo Moritake

The effect of the nitric oxide donor SIN-1 on the membrane potential of cultured mouse neuroblastoma-rat glioma hybrid NG108-15 cells was investigated using the whole cell patch method. It has been reported that neurite formation can be induced in NG108-15 cells by adding of dibutyryl cyclic AMP to the culture medium. Using this system we found that SIN-1 has a selective inhibitory effect on the membrane potential of the calcium current which is concentration-dependent in the 1 mu M-100 microM range. This effect was transient and reversible, the same as seen with the calcium channel blocker nilvadipine at concentrations of 10 microM to 10 microM. At higher concentrations, ranging from 500 microM to 1 mM, however, SIN-1 also caused prolonged inhibition of the membrane potential of the sodium current. However, this effect was also reversible. These findings suggest that SIN-1 has a reversible inhibitory action on the membrane potentials of neurons.

Neurite

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Mitomycin C-induced acceleration of liver cell polyploidization in adult rat after partial hepatectomy.

PubMed (1983)

Keiji Nakanishi Setsuya Fujita C. Pellicciari Yohei Hosokawa Yoji Urata

Mitomycin C, a DNA cross-linking agent, was administered for a week intraperitoneally to a normal 9-week-old rat in which hepatocyte proliferative activity had almost ceased. In the rat treated with mitomycin C, the number of polyploid cells with the DNA amounts more than 4C was not increased in comparison with that in the control rat without any treatment. However, the partial hepatectomy in the rat pretreated with mitomycin C provoked prominent hepatocyte polyploidization much greater in degree than that found in the hepatectomized rat without mitomycin C treatment. These results seem to indicate that the existence of cross-linking DNA damages is a latent factor necessary for the induction of abortive mitosis of a cell after completion of DNA synthesis, which results in the production of a mononuclear polyploid cell in one-step higher DNA class. Cross-linking DNA damages and DNA synthesis are the essential factors for the manifestation of polyploidization.

Mitomycin C

Polyploid

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Citations (3)

Elevated levels of 4-hydroxynonenal-histidine Michael adduct in the hippocampi of patients with Alzheimer's disease

Biomedical Research (2009)

Mitsugu Fukuda Fumihisa Kanou Nobuko Shimada Motoji Sawabe Yuko Saito

Alzheimer's disease (AD) is among the most common causes of progressive cognitive impairment in humans and is characterized by neurodegeneration in the brain. Lipid peroxidation is thought to play a role in the pathogenesis of AD. 4-hydroxynonenal (HNE) results from peroxidation of polyunsaturated fatty acids and it in turn gives evidence of lipid peroxidation in vivo. HNE reacts with protein histidine residue to form a stable HNE-histidine Michael adduct. To clarify the influence of lipid peroxidation on the pathogenesis of AD, we measured HNE-histidine Michael adduct in hippocampi from four AD patients and four age-matched controls by means of semiquantitative immunohistochemistry using a specific antibody to cyclic hemiacetal type of HNE-histidine Michael adduct. This antibody does not react with the ring-opened form of HNE-histidine Michael adduct and the pyrrole form of HNE-lysine Michael adduct. The HNE adduct was detected in the hippocampi of both AD and control donors, especially in the CA2, CA3 and CA4 sectors. Immunoreactive intensity of HNE adduct in these sectors were significantly higher in AD patients than in the controls. The HNE adduct was found in the perikarya of pyramidal cells in the hippocampus. These results show that the hippocampi of patients with AD undergo lipid peroxidation and imply that this activity underlies the production of cytotoxic products such as HNE that are responsible for the pathogenesis of AD.

4-Hydroxynonenal

Pathogenesis

10.2220/biomedres.30.227

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Citations (61)