Objective To evaluate the effect of Docetaxel eye drops on cornea neovascularization of rabbit induced by alkali cauterization.Methods Cornea neovascularization (CNV) was induced by 1 mol·l-1 NaOH in 24 rabbits.The rabbits were then divided into four groups,group A:10μg·ml-1 Docetaxel treated group (n=6),group B:20μg·ml-1 Docetaxel treated group (n=6),group C:40μg·ml-1 Docetaxel treated group (n=6),group D:control group (n=6),group A,B and C had been given the eye drops since the 2 days after alkali injury.The eyes in each group were examined by microscope at 3,7,14 days.The area of CNV was marked by photography.Immunohisto-chemistry and RT-PCR were used to determine the VEGF level.Results Docetaxel eye drops both inhibited the length and area of rabbit corneal neovascularization at different concentration.However,there was a little difference between the three concentrations of Docetaxel eye drops.The expression of VEGF was suppressed significantly in Docetaxel eye drops group,nevertheless the expression of VEGF was markedly increased in the control group.Conclusions Docetaxel is effective in the inhibition of corneal neovacularization induced by alkaline burn.
Key words:
Docetaxel; Corneal neovascularization; Endothelial growth factor
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