Won-Bum Lee

National University College

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Jaebeom Lee

Chungnam National University

Yoon-Seo Jang

Pusan National University

Ki‐Jae Jeong

National Center for Nanoscience and Technology

Cai‐Feng Wang

Nanjing Tech University

Jin-Young Park

Duke University

Jung-Han Lee

GS Caltex (South Korea)

Dae‐Seok Hwang

Pusan National University

Mi Heon Ryu

Asan Medical Center

Uk‐Kyu Kim

Pusan National University

Dae Youn Hwang

Pusan National University

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Pusan National University

Pusan National University Dental Hospital

Kyung Hee University

Chungnam National University

Pusan National University Hospital

National Medical Research Center of Dentistry and Maxillofacial Surgery

Pohang University of Science and Technology

Duke University

National Institute of Biological Resources

Weatherford College

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Whitlockite Granules on Bone Regeneration in Defect of Rat Calvaria

ACS Applied Bio Materials (2020)

Won-Bum Lee Cai‐Feng Wang Jung-Han Lee Ki‐Jae Jeong Yoon-Seo Jang

Whitlockite (WH; Ca18Mg2(HPO4)2(PO4)12) is a calcium phosphate based ceramic that contains magnesium ions. As the second most abundant mineral in living bone, WH occupies 25-35 wt % of the inorganic portion of human bone. Compared to hydroxyapatite (HAp, Ca10(PO4)6(OH)2), WH possesses better mechanical properties, faster resorbability, and promotion behavior on the osteogenesis. In this article, we introduced a fabrication method of interconnected porous WH granules through vacuum filtration, followed by sintering treatment based on the thermal stability of WH synthesized using the tri-solvent system. This study presents a histological, radiological, and immunohistochemical evaluation of the bone healing potential of these WH granules in a 5 mm diameter calvarial bone defect in rats. The histological evaluation shows no inflammation or foreign body reaction in the WH group. The WH group displays newly formed bone at the same thickness as the original bone. On the contrary, bone formation is not observed in the nontreated (NT) group. Besides, immunohistochemistry (IHC) confirmed that WH granules promoted bone regeneration with the significantly higher expression of bone morphogenetic proteins-2 (BMP-2), alkaline phosphatase (ALP), osteopontin (OPN), and osteocalcin (OCN) compared to the NT group without the addition of exogenous cells or growth factors. These results suggest that WH has excellent potential for application in bone tissue regeneration.

Calvaria

Whitlockite

Osteopontin

Bone tissue

10.1021/acsabm.0c00960

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법인 아닌 사단에 유추 적용되는지 여부와 종교단체에서의 제한 가능성 (대상결정 : 대법원 2009. 11. 19.자 2008마699 전원합의체 결정)

Won-Bum Lee

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Whitlockite nanoparticle-coated hydroxyapatite granules for bone regeneration in defect of rat calvaria

Research Square (Research Square) (2023)

Won-Bum Lee Cai‐Feng Wang Yoon-Seo Jang Ki‐Jae Jeong Hyo-Jin Kang

Abstract Background Whitlockite (WH; Ca 18 Mg 2 (HPO 4 ) 2 (PO 4 ) 12 ) as the second most abundant ceramic of human bone after hydroxyapatite (HA; Ca 10 (PO 4 ) 6 (OH) 2 ), possessing high osteogenic activity, has been known that it can stimulate osteogenic differentiation as well as suppress osteoclastic activity, particularly, in the preparatory stage of bone regeneration. Methods In this work, we construct a unique ceramic structure by layering WH on the surface of HA granules (HAGs)s via dip-coating and sintering method. Results The cell proliferation of the WH-coated HAGs (WHHAGs) group in the cell counting kit (CCK-8) was about 1.15-fold higher than that of the NT group at 72 h after incubation. The western blot and qPCR results demonstrated that WHHAGs treatment readily stimulated the transcription of osteogenic genes by regulating the downstream signaling pathway of BMP and WNT receptors.This study presents a histological and radiological evaluation of the bone healing potential of the WHHAGs in a 7 mm diameter calvarial bone defect in rats, compared with/without the BMP and non-treated (NT) groups at the 8 weeks after surgery. The bone volume fraction (bone volume/tissue volume) of the WHHAGs group was about 7.32- and 3.56- fold higher than that of the NT group with/without the BMP, respectively. Besides, histological evaluation confirmed that the WHHAGs with BMP promoted bone regeneration. Conclusion These results suggest that the WHHAGs present the remarkable potential for application in dental and orthopedic bone regeneration.

Calvaria

10.21203/rs.3.rs-3125669/v1

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