Yuqi Guo

Sichuan Agricultural University

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Dong Liang

Sichuan Agricultural University

Hui Xia

Sichuan Agricultural University

Honghong Deng

Sichuan Agricultural University

Lijin Lin

Sichuan Agricultural University

Xiulan Lv

Sichuan Agricultural University

Xinling Liu

Shanghai Normal University

Kunfu Xu

Sichuan Agricultural University

Qunxian Deng

Sichuan Agricultural University

Jin Wang

Hunan Agricultural University

Zhiyi Lin

Xiamen University Malaysia

Cooperative Institutions

Sichuan Agricultural University

Institute of Pomology

Chinese Academy of Sciences

Shanxi Agricultural University

Sichuan Provincial Academy of Natural Resource Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Sun Yat-sen University

University of Chinese Academy of Sciences

Texas A&M University

Sichuan Academy of Forestry

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Comparative analysis of flavonoids in white and red table grape cultivars during ripening by widely targeted metabolome and transcript levels

Journal of Food Science (2022)

Hui Xia Xuefeng Zhang Yanqiu Shen Yuqi Guo Tong Wang

The flavonoid metabolites were compared between red 'Summer Black' (SB) and white 'Shine Muscat' (SM) table grapes during fruit development based on widely targeted metabolome. A total of 134 flavonoids were identified in two cultivars, including 37 flavones, 33 flavonols, and 11 anthocyanidins, and so on. From young to veraison, the composition and the content of most flavonoids were decreasing in both cultivars but increased at maturation in SB. In general, SB has higher flavonoid compositions and content than SM during the whole fruit development, especially the content of anthocyanin after veraison. While the SM had higher content of flavonols such as quercetin, kaempferol and their derivatives. The expression of anthocyanin-related genes such as UFGT, OMT, GST, MATE, MYBA1, and MYBA2 was remarkably higher in SB than those in SM, which may attribute to higher anthocyanin content, while the higher expression of F3H and FLS resulted higher level of flavonols in SM. These results improve our understanding of flavonoid profiles and molecular mechanism in table grape cultivars.

Flavonols

Veraison

Metabolome

Flavones

Anthocyanidins

Table grape

10.1111/1750-3841.16117

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Citations (6)

AcMADS32 positively regulates carotenoid biosynthesis in kiwifruit by activating AcBCH1/2 expression

International Journal of Biological Macromolecules (2023)

Hui Xia Zhiyi Lin Zunzhen He Yuqi Guo Xinling Liu

Phytoene

10.1016/j.ijbiomac.2023.124928

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Citations (3)

Effects of Seawater on Carotenoid Production and Lipid Content of Engineered Saccharomyces cerevisiae

Fermentation (2019)

Yuqi Guo Shangxian Xie Joshua S. Yuan Katy C. Kao

The use of seawater in fermentation can potentially reduce the freshwater burden in the bio-based production of chemicals and fuels. We previously developed a Saccharomyces cerevisiae carotenoids hyperproducer SM14 capable of accumulating 18 mg g−1 DCW (DCW: dry cell weight) of β-carotene in rich media (YPD). In this work, the impacts of seawater on the carotenoid production of SM14 were investigated. When using nutrient-reduced media (0.1× YNB) in freshwater the β-carotene production of SM14 was 6.51 ± 0.37 mg g−1 DCW; however in synthetic seawater, the production was increased to 8.67 ± 0.62 mg g−1 DCW. We found that this improvement was partially due to the NaCl present in the synthetic seawater, since supplementation of 0.5 M NaCl in freshwater increased β-carotene production to 11.85 ± 0.77 mg g−1 DCW. The combination of synthetic seawater with higher carbon-to-nitrogen ratio (C:N = 50) further improved the β-carotene production to 10.44 ± 0.35 mg g−1 DCW. We further showed that the carotenoid production improvement in these conditions is related with lipid content and composition. These results demonstrated the benefit of using seawater to improve the production of carotenoids in S. cerevisiae, and have the potential to expand the utilization of seawater.

Carotene

Dry weight

10.3390/fermentation5010006

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Citations (8)

An efficient callus protoplasts isolation and PEG-mediated transient expression system for subcellular localization in kiwifruit

Research Square (Research Square) (2023)

Yuqi Guo Xinling Liu Yuxuan Lang Zunzhen He Meijing Wu

Abstract Protoplast isolation and transient gene expression have been served as valuable tools for gene function study in plants, however, they are rarely used in most woody plants due to the lack of efficient protoplast isolation system. In the present study, the protoplast isolation and purification system of kiwifruit callus was established after condition optimization. First, the loose kiwifruit callus were obtained using young true leaves as explants, inducing on MS medium containing 0.5 mg/L zeatin and 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and propagating on medium supplemented with 1.0 mg/L 2,4-D + 0.5 mg/L 6-benzylaminopurine + 0.5 mg/L 1-naphthlcetic acid. Then, the callus subcultured at 15 d were digested with enzyme solution containing 2.0% Cellulase R-10, 0.5% Macerozyme R-10, and 0.7 M mannitol for 7 h in the dark to obtain protoplasts, reaching the yield of 2.8 × 10 6 protoplasts·g −1 FW and the viability up to 87%. In addition, transient transformation condition in kiwifruit protoplast were optimized, approximately 40% transfection efficiency was obtained with 40% PEG4000 and 15 min transfection duration. By this way, the subcellular localization of AcMYB6l-GFP fusion proteins was verified. Taken together, we developed an efficient protocol for protoplast isolation and transient transfection in kiwifruit, laying a foundation for future research on gene function and molecular breeding in Actindia .

Protoplast

Callus

Zeatin

10.21203/rs.3.rs-2966782/v1

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Subzero-temperature homogeneous liquid–liquid extraction for the stereoselective determination of chiral triadimefon and its metabolite in water, fruit juice, vinegar, and fermented liquor by HPLC

Analytical Methods (2023)

Xingle Guo Haijuan Jiang Yuqi Guo Liyan Jia Xu Jing

A novel method based on homogeneous liquid–liquid extraction with deep eutectic solvents under subzero-temperature conditions in combination with HPLC for the determination of chiral fungicide triadimefon and its metabolite triadimenol was developed.

Triadimefon

10.1039/d3ay01061a

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Citations (1)

Dynamic Changes in Ascorbic Acid Content during Fruit Development and Ripening of Actinidia latifolia (an Ascorbate-Rich Fruit Crop) and the Associated Molecular Mechanisms

International Journal of Molecular Sciences (2022)

Honghong Deng Hui Xia Yuqi Guo Xinling Liu Lijin Lin

Actinidia latifolia is one of the very few kiwifruit genotypes with extremely high ascorbic acid (AsA) content. However, a transcriptome atlas of this species is lacking. The accumulation of AsA during fruit development and ripening and the associated molecular mechanisms are still poorly understood. Herein, dynamic changes in AsA content at six different stages of A. latifolia fruit development and ripening were determined. AsA content of A. latifolia fruit reached 1108.76 ± 35.26 mg 100 g-1 FW at full maturity. A high-quality, full-length (FL) transcriptome of A. latifolia was successfully constructed for the first time using third-generation sequencing technology. The transcriptome comprises 326,926 FL non-chimeric reads, 15,505 coding sequences, 2882 transcription factors, 18,797 simple sequence repeats, 3328 long noncoding RNAs, and 231 alternative splicing events. The genes involved in AsA biosynthesis and recycling pathways were identified and compared with those in different kiwifruit genotypes. The correlation between the AsA content and expression levels of key genes in AsA biosynthesis and recycling pathways was revealed. LncRNAs that participate in AsA-related gene expression regulation were also identified. Gene expression patterns in AsA biosynthesis and metabolism exhibited a trend similar to that of AsA accumulation. Overall, this study paves the way for genetic engineering to develop kiwifruits with super-high AsA content.

Actinidia

Actinidia chinensis

10.3390/ijms23105808

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Citations (18)

Insights on Osmotic Tolerance Mechanisms in Escherichia coli Gained from an rpoC Mutation

Bioengineering (2017)

Yuqi Guo James D. Winkler Katy C. Kao

An 84 bp in-frame duplication (K370_A396dup) within the rpoC subunit of RNA polymerase was found in two independent mutants selected during an adaptive laboratory evolution experiment under osmotic stress in Escherichia coli, suggesting that this mutation confers improved osmotic tolerance. To determine the role this mutation in rpoC plays in osmotic tolerance, we reconstructed the mutation in BW25113, and found it to confer improved tolerance to hyperosmotic stress. Metabolite analysis, exogenous supplementation assays, and cell membrane damage analysis suggest that the mechanism of improved osmotic tolerance by this rpoC mutation may be related to the higher production of acetic acid and amino acids such as proline, and increased membrane integrity in the presence of NaCl stress in exponential phase cells. Transcriptional analysis led to the findings that the overexpression of methionine related genes metK and mmuP improves osmotic tolerance in BW25113. Furthermore, deletion of a stress related gene bolA was found to confer enhanced osmotic tolerance in BW25113 and MG1655. These findings expand our current understanding of osmotic tolerance in E. coli, and have the potential to expand the utilization of high saline feedstocks and water sources in microbial fermentation.

Osmotic shock

Osmotic concentration

10.3390/bioengineering4030061

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Citations (17)

Identification of Suitable Reference Genes for qRT-PCR Normalization in Kiwifruit

Horticulturae (2022)

Yuanjie Zhou Hui Xia Xinling Liu Zhiyi Lin Yuqi Guo

Reference genes are used for the correction of qRT-PCR data, and it is necessary to investigate the optimum reference gene under certain conditions. The expression levels of seven traditional reference genes ACT1, ACT2, GAPDH, 18S rRNA, UBQ, TUB and CYP were analyzed using qRT-PCR in different varieties, tissues, developmental stages and hormone (or pollen polysaccharide) treatments in kiwifruit. Gene expression stability was assessed with the help of three common software (geNorm, NormFinder, BestKeeper), and the minimum number of reference genes necessary for normalization was also determined. GAPDH, ACT1 and ACT2 were selected as reference genes for different genotypes of kiwifruit. GAPDH and UBQ were the best combinations of reference genes for root, stem, leaf, flower and fruit. GAPDH and ACT1 could be the preferred reference genes for normalization of qRT-PCR data during fruit development. The pairing of ACT1 and UBQ constituted the optimal combination of reference genes in kiwifruit treated with different hormones (or pollen polysaccharide). This study provides a new and reliable option for the use of reference genes in the analysis of gene expression patterns of interest in kiwifruit.

Reference Genes

Normalization

Glyceraldehyde 3-phosphate dehydrogenase

10.3390/horticulturae8020170

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Citations (8)

Methylation of AcGST1 Is Associated with Anthocyanin Accumulation in the Kiwifruit Outer Pericarp

Journal of Agricultural and Food Chemistry (2023)

Xuefeng Zhang Kun Zhang Yuqi Guo Xiaoyu Lv Meijing Wu

Most red-fleshed kiwifruit cultivars, such as Hongyang, only accumulate anthocyanins in the inner pericarp; the trait of full red flesh becomes the goal pursued by breeders. In this study, we identified a mutant "H-16" showing a red color in both the inner and outer pericarps, and the underlying mechanism was explored. Through transcriptome analysis, a key differentially expressed gene AcGST1 was screened out, which was positively correlated with anthocyanin accumulation in the outer pericarp. The result of McrBC-PCR and bisulfite sequencing revealed that the SG3 region (−292 to −597 bp) of AcGST1 promoter in "H-16" had a significantly lower CHH cytosine methylation level than that in Hongyang, accompanied by low expression of methyltransferase genes (MET1 and CMT2) and high expression of demethylase genes (ROS1 and DML1). Transient calli transformation confirmed that demethylase gene DML1 can activate transcription of AcGST1 to enhance its expression. Overexpression of AcGST1 enhanced the anthocyanin accumulation in the fruit flesh and leaves of the transgenic lines. These results suggested that a decrease in the methylation level of the AcGST1 promoter may contribute to accumulation of anthocyanin in the outer pericarp of "H-16".

Demethylase

Flesh

Bisulfite sequencing

10.1021/acs.jafc.3c03029

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Citations (5)

Insight on Mechanisms of Osmotic Tolerance

Yuqi Guo

Source

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