Techniques for microsampling in infrared spectroscopy by matrix isolation are described. In the matrix isolation of liquid or solid compounds, the sample is vaporized and then diluted with a large excess of a matrix gas; the resulting gaseous mixture is deposited on a cold surface for spectroscopic examination as a solid. Detailed descriptions of the apparatus and procedures employed in the preparation of matrix-isolated samples for qualitative and quantitative analyses by Fourier transform infrared spectrometry are presented. Systems for “microsampling” (detection limits for specific compounds ∼0.5 to 1 μg) and “ultramicrosampling” (detection limits ∼50 to 100 ng) matrix isolation spectroscopy are described. Detection limits and Beer's law behavior for polycyclic aromatic hydrocarbons microsampled by matrix isolation are discussed. The advantages of matrix isolation as a technique for qualitative and quantitative infrared analysis of microsamples are itemized.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMatrix isolation Fourier transform infrared spectrometry of polycyclic aromatic hydrocarbonsGleb. Mamantov, E. L. Wehry, R. R. Kemmerer, and E. R. HintonCite this: Anal. Chem. 1977, 49, 1, 86–90Publication Date (Print):January 1, 1977Publication History Published online1 May 2002Published inissue 1 January 1977https://pubs.acs.org/doi/10.1021/ac50009a032https://doi.org/10.1021/ac50009a032research-articleACS PublicationsRequest reuse permissionsArticle Views129Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Mixtures of polycyclic aromatic hydrocarbons (PAH) as found in naturally occurring oils and petroleum, or synthetic oils produced by coal conversion processes, are too complex for characterization by any single technique. The approach taken frequently is to apply preliminary separation procedures of various kinds, including distillation, extraction, and chromatographic methods, then attempt to analyze the separated fractions by a high resolution technique. In this chapter we describe the liquid chromatographic separation of mixtures of PAH using poly(vinyl pyrrolidone) (PVP) as the stationary phase and isopropyl alcohol as eluant. The resulting fractions are examined by matrix isolation (MI) Fourier transform infrared (FTIR) and fluorescence spectroscopy.
