This study involves the synthesis of hydroxyapatite and describes the preparation and characterization of polymer coatings based on poly(ethylene glycol) diacrylate and poly(ethylene glycol) and modified with bovine serum albumin and hydroxyapatite. Hydroxyapatite was obtained by wet chemical synthesis and characterized by X-ray diffraction and FTIR spectroscopy, and its Ca/P molar ratio was determined (1.69 ± 0.08). The ceramic and bovine serum albumin were used in the preparation of composite materials with the polymeric matrix. The chemical composition of coatings was characterized with FTIR spectroscopy, and their morphology was recorded with SEM imaging. Moreover, the measurements of surface roughness parameters and stereometric research were performed. The prepared coatings were subjected to in vitro studies in simulated body fluid and artificial saliva. Changes in chemical composition and morphology after immersion were examined with FTIR spectroscopy and SEM imaging. Based on the conducted research, it can be stated that applied modifiers promote the biomineralization process. The roughness analysis confirmed prepared materials were characterized by the micrometer-scale topography. The materials morphology and roughness, and the morphology of the newly formed apatite deposit, were dependent on the type of the used modifier, and the artificial fluid used in in vitro studies.
Sodium alginate/poly(vinyl alcohol) hydrogels containing Echinacea purpurea extract (0–20%) with antioxidant activity (73.2 ± 2.4% inhibition, 2.54 ± 0.096 TEAC) were developed using UV radiation. The swelling ability increases with increased extract contents and it reaches about 300%, which is related to the porosity. The presence of 20% of extract directly impacted the morphology, which became more porous. The degradation tests indicate no significant differences in pH and conductivity. However, the gradual increase of conductivity exhibits the expected rate of material degradation over time. In the case of hydrogels containing 5% and 15% of extract, the analyzed echinacoside was being released during 6 days.
Event Abstract Back to Event In vitro cytotoxicity of silver nanoparticles as a hydroxyapatite modifier on human fibroblasts Agnieszka Sobczak-Kupiec1, Klaudia Pluta1, Dagmara Malina1, Katarzyna Bialik-Wąs2 and Bożena Tyliszczak2 1 Institute of Inorganic Chemistry and Technology, Cracow University of Technology, Poland 2 Department of Chemistry and Technology of Polymers, Cracow University of Technology, Poland Biocompatible, bioactive and osteoconductive hydroxyapatite of general formula [HAp, Ca10(PO4)6(OH)2] is the most promising material used widely in medicine. Notably, HAp is extensively used as a material for regeneration of tissue defects caused by resection of the tumor or fractures as a consequence of osteoporosis as well as in dentistry. Hydroxyapatites modified with silver nanoparticles of enhanced antibacterial properties are of great interest in the development of new products. The main goal of this study was to evaluate the potential toxic cellular effects of different concentration of silver nanoparticles suspension on human fibroblasts. The following materials were used in experiments: fibroblast cell line, BJ (ATCC, CRL-2522TM, from the foreskin of newborn with PN-EN ISO 10993-5:2009 standards which was grown in DMEM supplemented with fetal calf serum (FBS, 10% (v/v). Cytotoxicity was evaluated with MTT assay. Silver nanoparticles were synthesized by chemical reduction using aqueous solution of polyvinylpyrrolidone as a stabilizing agent. Sample denoted as 1 is comprised of silver nanoparticles where sodium borohydride was used as reducing agent, samples 2 denotes 1:1 ratio between silver ions and reducing agent and accordingly sample 3 denotes 1:10 ratio between silver ions and reducing agent. The Figure 1 shows the results of suspensions of silver nanoparticles exposed to cell viability dermis after 24 hours incubation in 37˚C. Figure 1 exemplify also correlation between relative values of samples determined in respect to control samples. The viability of cells for each sample is expressed as relative value estimated with respect to sample that contain the suspension of nanoparticles. Figure 1. Viability of fibroblasts after 24h exposure to varying concentrations of AgNPs determined by MTT assay The study showed the correlation between concentration of silver nanoparticles and viability of dermis cells. With increasing concentrations of AgNPs suspensions, cell viability decreases. The analysis showed no significant differences in viability of cells when using different reducing agents for synthesis of silver nanoparticles. This work was financed by the National Centre for Research and Development under the Lider project contract no. 037/481/L-5/13/NCBR/2014References:[1] Rumpel E., Wolf E., Kauschke E., Bienengräber V., Folia Morphol. Vol. 65, No. 1, pp. 43–48. Keywords: in vitro, cell, Cell response Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regeneration inducing biomaterials Citation: Sobczak-Kupiec A, Pluta K, Malina D, Bialik-Wąs K and Tyliszczak B (2016). In vitro cytotoxicity of silver nanoparticles as a hydroxyapatite modifier on human fibroblasts. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00621 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Agnieszka Sobczak-Kupiec Klaudia Pluta Dagmara Malina Katarzyna Bialik-Wąs Bożena Tyliszczak Google Agnieszka Sobczak-Kupiec Klaudia Pluta Dagmara Malina Katarzyna Bialik-Wąs Bożena Tyliszczak Google Scholar Agnieszka Sobczak-Kupiec Klaudia Pluta Dagmara Malina Katarzyna Bialik-Wąs Bożena Tyliszczak PubMed Agnieszka Sobczak-Kupiec Klaudia Pluta Dagmara Malina Katarzyna Bialik-Wąs Bożena Tyliszczak Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
