Florence Aviat

Infection et inflammation

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Michel Fédérighi

Sécurité des Aliments et Microbiologie

Christophe Belloncle

École Supérieure du Bois

Hélène Pailhories

Université d'Angers

Matthieu Eveillard

Centre Hospitalier Universitaire d'Angers

Laurence Dubreil

Nerve Centre

Muhammad Tanveer Munir

Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail

Mark Irle

École Supérieure du Bois

Isabelle Le Bayon

Institut Technologique Forêt Cellulose Bois-Construction Ameublement

Rached Ismaïl

Laboratoire des Matériaux Avancés

G. André‐Fontaine

Oniris

Cooperative Institutions

Biology of Infection

129

Engineering Associates (United States)

123

New York Proton Center

Oregon Medical Research Center

Communities In Schools of Orange County

Lindsay Unified School District

University of Pittsburgh

Manhattan Institute for Policy Research

Museum of Heilongjiang Province

Oniris

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Confocal spectral microscopy, a non-destructive approach to follow contamination and biofilm formation of mCherry Staphylococcus aureus on solid surfaces

Scientific Reports (2021)

Muhammad Tanveer Munir Nattar Maneewan Julien Pichon Mohammed Gharbia Ismael Oumarou-Mahamane

Abstract Methods to test the safety of wood material for hygienically sensitive places are indirect, destructive and limited to incomplete microbial recovery via swabbing, brushing and elution-based techniques. Therefore, we chose mCherry Staphylococcus aureus as a model bacterium for solid and porous surface contamination. Confocal spectral laser microscope (CSLM) was employed to characterize and use the autofluorescence of Sessile oak ( Quercus petraea ), Douglas fir ( Pseudotsuga menziesii ) and poplar ( Populus euramericana alba L.) wood discs cut into transversal (RT) and tangential (LT) planes. The red fluorescent area occupied by bacteria was differentiated from that of wood, which represented the bacterial quantification, survival and bio-distribution on surfaces from one hour to one week after inoculation. More bacteria were present near the surface on LT face wood as compared to RT and they persisted throughout the study period. Furthermore, this innovative methodology identified that S. aureus formed a dense biofilm on melamine but not on oak wood in similar inoculation and growth conditions. Conclusively, the endogenous fluorescence of materials and the model bacterium permitted direct quantification of surface contamination by using CSLM and it is a promising tool for hygienic safety evaluation.

Autofluorescence

10.1038/s41598-021-94939-2

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Citations (5)

An assessment of L. monocytogenes transfer from wooden ripening shelves to cheeses: Comparison with glass and plastic surfaces

Food Control (2016)

Rached Ismaïl Florence Aviat Perrine Gay-Perret Isabelle Le Bayon Michel Fédérighi

10.1016/j.foodcont.2016.08.014

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Citations (18)

ICE volume 38 issue 3 Cover and Front matter

Infection Control and Hospital Epidemiology (2017)

Jennifer Dela‐Pena Luiza Kerstenetzky Lucas Schulz Ron Kendall Alexander J. Lepak

An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Front cover

10.1017/ice.2017.14

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Citations (1)

Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis

Journal of Medical Microbiology (2009)

Kristel Lourdault Florence Aviat Mathieu Picardeau

The dynamics of leptospirosis infection have been poorly studied. The purpose of this study was to determine the LD 50 , rate of bacterial dissemination, histopathology and antibody responses against leptospira following inoculation with the highly virulent Leptospira interrogans Fiocruz L1-130 strain in a guinea pig model of leptospirosis. Three routes of infection (intraperitoneal, conjunctival and subcutaneous inoculation) were used to establish disease in guinea pigs. The size and kinetics of leptospiral burdens in the blood and tissues of infected animals were determined over a 1 week course of infection using quantitative real-time PCR (qPCR). Bacteraemia peaked at day 5 post-infection reaching more than 5×10 4 leptospires ml −1 . The highest spirochaetal load was found in the liver and kidneys, and was associated with alterations in organ tissues and a decline in liver and kidney functions. In contrast, lesions and bacteria were not detected in guinea pigs infected with an avirulent strain derived from a high-passage-number in vitro -passaged variant of the Fiocruz L1-130 strain. The use of qPCR supports the findings of earlier studies and provides an easy and reliable method for the quantification of L. interrogans in the tissues of infected animals. qPCR will be used in future studies to evaluate the efficacy of vaccine candidates against leptospirosis and the virulence of selected L. interrogans mutants relative to the parental strain.

Leptospira interrogans

Histopathology

10.1099/jmm.0.008169-0

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Citations (61)

Protection againstLeptospira interrogansSensu Lato Challenge by DNA Immunization with the Gene Encoding Hemolysin-Associated Protein 1

Infection and Immunity (2005)

C. Branger B. Chatrenet Anne Gauvrit Florence Aviat André Aubert

The use of DNA constructs encoding leptospiral proteins is a promising new approach for vaccination against leptospirosis. In previous work we determined that immunization with hemolysis-associated protein 1 (Hap1) (LipL32) expressed by adenovirus induced significant protection against a virulent Leptospira challenge in gerbils. To avoid the use of the adenovirus vector, we checked for clinical protection against lethal challenge by DNA vaccination. A DNA vaccine expressing Hap1 was designed to enhance the direct gene transfer of this protein into gerbils. A challenge was performed 3 weeks after the last immunization with a virulent strain of serovar canicola. Our results show that the cross-protective effect with pathogenic strains of Leptospira, shared by Hap1, could be mediated by the DNA plasmid vector. This finding should facilitate the design and development of a new generation of vaccines against bacteria, particularly Leptospira interrogans sensu lato.

Leptospira interrogans

Hemolysin

10.1128/iai.73.7.4062-4069.2005

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Citations (100)

The nondestructive microscopic imaging of hygienically important microbes and contaminants on wood

HAL (Le Centre pour la Communication Scientifique Directe) (2019)

Munir Tanveer Michel Fédérighi Florence Aviat Ismaël Oumarou Mahamane Hélène Pailhories

Source

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Direct screening method to assess antimicrobial behavior of untreated wood

European Journal of Wood and Wood Products (2019)

Muhammad Tanveer Munir Florence Aviat Hélène Pailhories Matthieu Eveillard Mark Irle

Sawdust

Agar diffusion test

10.1007/s00107-019-01390-2

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Citations (15)

Antimicrobial Characteristics of Untreated Wood: Towards a Hygienic Environment

Health (2019)

Muhammad Tanveer Munir Hélène Pailhoriès Matthieu Eveillard Florence Aviat Didier Lepelletier

Wood, as a contact surface, has been used for centuries but is usually questioned because of its porosity and organic composition. It has natural antimicrobial properties and, hygienically, can stand the comparison with other materials such as plastic, glass and steel. In this review, we focused on potential microbe-inhibiting properties of wooden surfaces being used in hygienically important places like health institutes and food industries. This article addresses the questionable properties of untreated wood like hygroscopicity, porosity, roughness and chemical composition, and their relation to the hygienic and antimicrobial nature of this material. The other factors linked to the hygienic properties of wood, such as age, species and type of wood, have also been discussed. Our analysis of literature will create better understanding for acceptance of wood as a safety renewable resource. It also provides an outline for future research considering wood material in critical healthcare or food industries.

Natural materials

Renewable resource

10.4236/health.2019.112014

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Citations (25)

Assessment of Penicillium expansum and Escherichia coli transfer from poplar crates to apples

Food Control (2015)

Mathilde Montibus Rached Ismaïl Valérie Michel Michel Fédérighi Florence Aviat

Blue mold

10.1016/j.foodcont.2015.07.025

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Citations (18)

Survival of Bacterial Strains on Wood (Quercus petraea) Compared to Polycarbonate, Aluminum and Stainless Steel

Antibiotics (2020)

Ju-Chi Chen Muhammad Tanveer Munir Florence Aviat Didier Lepelletier Patrice Le Pape

Healthcare-associated infections (HAI) remain a burden in healthcare facilities, environmental surfaces being a potential reservoir for healthcare-associated pathogens. In this context, exploration of materials with potential antimicrobial activities represents a way forward for the future. Here, we explored the survival of four bacterial species commonly involved in HAI (Acinetobacter baumannii, Enterococcus faecalis, Klebsiella pneumoniae, Staphylococcus aureus), on oak versus three other materials (aluminum, polycarbonate, stainless steel). Twenty microliters of each bacterial suspension (approximatively 107 bacteria) were deposited on each material. Bacterial counts were measured by grinding and culturing on day 0, 1, 2, 6, 7 and 15. Analyses were performed in triplicate for each material and each time evaluated. It appeared that the bacteria viable count decreased rapidly on transversal and tangential oak compared with the other materials for all bacterial species. Furthermore, no difference was noticed between transversal and tangential oak. These results underline the potential for use of oak materials in healthcare facilities, a consideration that should be supported by further investigations.

Enterococcus faecalis

Acinetobacter baumannii

Polycarbonate

10.3390/antibiotics9110804

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Citations (11)