James T. Slama

University of Toledo

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Timothy F. Walseth

University of Minnesota

Jonathan S. Marchant

Medical College of Wisconsin

Katherine A. Wall

University of Toledo

Gihan S. Gunaratne

Medical College of Wisconsin

Anne M. Simmons

University of Toledo

David R. Giovannucci

University of Toledo

Myron K. Jacobson

University of Arizona

Sandip Patel

University College London

Ramadan A. Ali

Ain Shams University

Eugen Brailoiu

Temple University

Cooperative Institutions

University of Toledo

University of Kentucky

University of Toledo Medical Center

University of Minnesota

University College London

National Institutes of Health

University of Michigan–Ann Arbor

University of Arizona

The University of Texas Health Science Center at San Antonio

Medical College of Wisconsin

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Progesterone receptor membrane component 1 facilitates Ca2+ signal amplification between endosomes and the endoplasmic reticulum

Journal of Biological Chemistry (2023)

Gihan S. Gunaratne Sushil Kumar Yaping Lin-Moshier James T. Slama Eugen Brailoiu

Membrane contact sites (MCSs) between endosomes and the endoplasmic reticulum (ER) are thought to act as specialized trigger zones for Ca2+ signaling, where local Ca2+ released via endolysosomal ion channels is amplified by ER Ca2+-sensitive Ca2+ channels into global Ca2+ signals. Such amplification is integral to the action of the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). However, functional regulators of inter-organellar Ca2+ crosstalk between endosomes and the ER remain poorly defined. Here, we identify progesterone receptor membrane component 1 (PGRMC1), an ER transmembrane protein that undergoes a unique heme-dependent dimerization, as an interactor of the endosomal two pore channel, TPC1. NAADP-dependent Ca2+ signals were potentiated by PGRMC1 overexpression through enhanced functional coupling between endosomal and ER Ca2+ stores and inhibited upon PGRMC1 knockdown. Point mutants in PGMRC1 or pharmacological manipulations that reduced its interaction with TPC1 were without effect. PGRMC1 therefore serves as a TPC1 interactor that regulates ER-endosomal coupling with functional implications for cellular Ca2+ dynamics and potentially the distribution of heme.

Component (thermodynamics)

10.1016/j.jbc.2023.105378

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Citations (4)

Structural Basis for Nicotinamide Inhibition and Base Exchange in Sir2 Enzymes

Molecular Cell (2007)

Brandi D. Sanders Kehao Zhao James T. Slama Ronen Marmorstein

Niacinamide

Nicotinamide mononucleotide

SIRT2

10.1016/j.molcel.2006.12.022

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Citations (62)

ChemInform Abstract: SEMISYNTHETIC ENZYMES: SYNTHESIS OF A NEW FLAVOPAPAIN WITH HIGH CATALYTIC EFFICIENCY

Chemischer Informationsdienst (1982)

James T. Slama S. Oruganti E. T. Kaiser

Abstract Nach Trifluoracetylierung von (Ia) bei 0°C in CF 3 COOH läßt sich das erhaltene (Ib) mit CH 3 I methylieren, so daß nach Abtrennen der Schutzgruppe das Aminonitroacetophenon (Ic) vorliegt.

10.1002/chin.198203313

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PXR and the regulation of apoA1 and HDL-cholesterol in rodents

Pharmacological Research (2004)

K A Bachmann Hiral Patel Zaid Batayneh James T. Slama David A. White

Pregnane X receptor

CYP3A

10.1016/j.phrs.2004.03.005

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Citations (68)

NAADP evokes calcium signals and plays a critical role in downstream physiological responses in naïve T cells (1013.5)

The FASEB Journal (2014)

Ramadan A. Ali Christina Camick Katherine Wiles James T. Slama David R. Giovannucci

Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent Ca 2+ mobilizing second messenger discovered to date and has been implicated in Ca 2+ signaling in human Jurkat lymphoma T cells via ryanodine receptor activation. In contrast the role of NAADP in Ca 2+ signaling or the identity of the Ca 2+ stores targeted by NAADP in conventional naïve T cells has not been investigated. In the current study, we demonstrated for the first time to our knowledge the importance of NAADP in the generation of Ca 2+ signals in murine conventional naïve T cells. Combining live‐cell imaging methods and a pharmacological approach using the NAADP antagonist Ned‐19, we addressed the impact of NAADP on the generation of Ca 2+ signals evoked by TCR stimulation and the role of this signal in downstream physiological endpoints such as proliferation, cytokine production, and other responses to stimulation of murine naïve T cells. Moreover, we demonstrated that acidic stores in addition to the endoplasmic reticulum are the relevant Ca 2+ stores that were sensitive to NAADP in naïve T cells. Grant Funding Source : Supported by NIH GM100444

Jurkat cells

Second messenger system

Calcium Signaling

10.1096/fasebj.28.1_supplement.1013.5

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Hst2 bound to ADP-HPD and Acetylated histone H4

Ronen Marmorstein Brandi D. Sanders Keji Zhao James T. Slama

10.2210/pdb2qqf/pdb

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ChemInform Abstract: SEMISYNTHETIC ENZYMES: CHARACTERIZATION OF ISOMERIC FLAVOPAPAINS WITH WIDELY DIFFERENT CATALYTIC EFFICIENCIES

Chemischer Informationsdienst (1985)

James T. Slama Czeslaw Radziejewski S. Oruganti E. T. Kaiser

Abstract Die Ketone (Ia) bzw. (IIa) werden über die Stufen (Ib)/(IIb), (lc)/(Ilc), (IVa)/(Va) in die Bromacetyl‐methylisoalloxazine (IVb) bzw. (Vb) übergeführt, mit denen der 25‐Cystein‐Baustein von Papain ‐ die enzymatische Substratbindungsstelle ‐ alkyliert wird.

Characterization

10.1002/chin.198508350

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Nicotinic Acid Adenine Dinucleotide Phosphate Analogues Substituted on the Nicotinic Acid and Adenine Ribosides. Effects on ReceptorMediated Ca²⁺ Release

Journal of Medicinal Chemistry (2015)

Christopher Trabbic Fan Zhang Timothy F. Walseth James T. Slama

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a Ca2+ releasing intracellular second messenger in both mammals and echinoderms. We report that large functionalized substituents introduced at the nicotinic acid 5-position are recognized by the sea urchin receptor, albeit with a 20–500-fold loss in agonist potency. 5-(3-Azidopropyl)-NAADP was shown to release Ca2+ with an EC50 of 31 μM and to compete with NAADP for receptor binding with an IC50 of 56 nM. Attachment of charged groups to the nicotinic acid of NAADP is associated with loss of activity, suggesting that the nicotinate riboside moiety is recognized as a neutral zwitterion. Substituents (Br– and N3−) can be introduced at the 8-adenosyl position of NAADP while preserving high potency and agonist efficacy and an NAADP derivative substituted at both the 5-position of the nicotinic acid and at the 8-adenosyl position was also recognized although the agonist potency was significantly reduced.

Moiety

10.1021/acs.jmedchem.5b00279

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Citations (15)

CCDC 606903: Experimental Crystal Structure Determination

The Cambridge Structural Database (2007)

James T. Slama N. Mehta Jerzy Jankun

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

10.5517/ccncjj1

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Synthesis and properties of photoaffinity labels for the pyridine dinucleotide binding site of NAD glycohydrolase

Biochemistry (1991)

James T. Slama Anne M. Simmons

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and properties of photoaffinity labels for the pyridine dinucleotide binding site of NAD glycohydrolaseJames T. Slama and Anne M. SimmonsCite this: Biochemistry 1991, 30, 9, 2527–2534Publication Date (Print):March 1, 1991Publication History Published online1 May 2002Published inissue 1 March 1991https://doi.org/10.1021/bi00223a033RIGHTS & PERMISSIONSArticle Views115Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (950 KB) Get e-Alerts Get e-Alerts

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10.1021/bi00223a033

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Citations (5)