Maria Assunta Costa

Instituto Biofisika

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V. Izzo

Istituto Nazionale di Fisica Nucleare, Sezione di Napoli

Giovanni Duro

Institute for Biomedical Research and Innovation

Rosa Passantino

Instituto Biofisika

Paolo Colombo

National Research Council

Domenico Geraci

Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy

Giovanni Locorotondo

Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy

Daniela Giacomazza

Instituto Biofisika

Roberta Cocchiara

Instituto Biofisika

Pier Luigi San Biagio

National Research Council

Maria Rosalia Mangione

National Research Council

Cooperative Institutions

University of Palermo

National Research Council

Instituto Biofisika

Istituto di Biomedicina e di Immunologia Molecolare Alberto Monroy

Institute for Biomedical Research and Innovation

National Academies of Sciences, Engineering, and Medicine

University of Naples Federico II

University of Palermo

University of Messina

Istituto di Farmacologia Traslazionale

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Biochemical and biophysical characterization of water-soluble pectin from Opuntia ficus-indica and its potential cytotoxic activity

Phytochemistry (2018)

Khalef Lefsih Daniela Giacomazza Rosa Passantino Maria Assunta Costa Donatella Bulone

Pectin

Ficus

Chemical structure

10.1016/j.phytochem.2018.06.015

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Citations (17)

Investigation on a MMACHC mutant from cblC disease: The c.394C>T variant

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics (2022)

Rosa Passantino Maria Rosalia Mangione María Grazia Ortore Maria Assunta Costa Alessia Provenzano

Homocystinuria

10.1016/j.bbapap.2022.140793

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Citations (1)

Parietaria pollen allergens

Allergy (1998)

Paolo Colombo Giovanni Duro Maria Assunta Costa V. Izzo Mario G. Mirisola

10.1111/j.1398-9995.1998.tb03791.x

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Citations (18)

New Perspectives for Watering Substrate-Based Root Modules in Microgravity in the Advanced Plant Habitat (APH)

Oscar Monje Jeffrey T. Richards DAVID HANSON M. M. Turpin Syed Moinuddin

The Advanced Plant Habitat (APH) was installed on the International Space Station (ISS) in October 2017. Following a successful EVT (Experiment Verification Test) study at Kennedy Space Center (KSC), using Arabidopsis lines with varying levels of lignin, two inaugural studies were carried out on ISS in 2018 under the same experimental design, with the corresponding ground controls at KSC. The APH for this study deploys a substrate-based root module designed for plant growth in microgravity. Upon experiment initiation (such as for the EVT), the root module is primed (liquid imbibition) by flooding the root zone to initiate seed germination and to remove air from the porous tubing and particulate media. In the APH ISS inaugural study, the speed of supplying water to initially dry media was found to adversely affect the overall moisture distribution within the root module in microgravity (but not at 1g). Non-destructive estimations of Arabidopsis plant growth were carried out by monitoring changes in rosette leaf area on a daily basis. These data indicated that the original priming procedure caused patchy moisture distribution that affected plant growth and survival. An improved methodology for priming the second root module of PH-01 was devised and implemented in the second experiment. Leaf area and color estimates suggested that the modified priming scheme improved moisture distribution and plant growth. These data, when compared with the EVT study, suggest that nondestructive measurements of plant growth can aid towards optimization of plant growth conditions in microgravity.

Priming (agriculture)

International Space Station

Imbibition

Source

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Citations (1)

Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels

Analytical Biochemistry (1993)

Roberto Barbieri V. Izzo Maria Assunta Costa Giorgio Lo Giudice Giovanni Duro

Agarose

Buffer (optical fiber)

Agarose gel electrophoresis

10.1006/abio.1993.1308

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Citations (15)

Multiple IgE recognition on the major allergen of the Parietaria pollen Par j 2

Molecular Immunology (2014)

Valeria Longo Maria Assunta Costa Fabio Cibella Giuseppina Cuttitta Stefania La Grutta

Epitope mapping

10.1016/j.molimm.2014.09.012

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Citations (10)

cDNA cloning, expression and primary structure of Par j I, a major allergen of Parietaria judaica pollen

FEBS Letters (1994)

Maria Assunta Costa Paolo Colombo V. Izzo Hannah Kennedy Silvana Venturella

A 659 bp cDNA clone 1 coding for an allergen of Pj pollen has been isolated from a lambda gt11 library, and its DNA sequence determined. The cDNA insert showed an open reading frame of 429 bp coding for an allergenic protein of 14,866 Da and a deduced amino acid sequence containing 143 residues. The expressed recombinant protein represented the major allergen Par j I since it reacted with 95% of the sera from Pj‐allergic patients ( n = 22) and with two Par j I‐specific monoclonal antibodies. No similarity with other known DNA and protein sequences has been detected.

Cloning (programming)

Coding region

10.1016/0014-5793(94)80453-2

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Citations (67)

Valorization of Apple Peels through the Study of the Effects on the Amyloid Aggregation Process of κ-Casein

Molecules (2021)

Valeria Guarrasi Giacoma Cinzia Rappa Maria Assunta Costa Fabio Librizzi Marco Raimondo

Waste valorization represents one of the main social challenges when promoting a circular economy and environmental sustainability. Here, we evaluated the effect of the polyphenols extracted from apple peels, normally disposed of as waste, on the amyloid aggregation process of κ-casein from bovine milk, a well-used amyloidogenic model system. The effect of the apple peel extract on protein aggregation was examined using a thioflavin T fluorescence assay, Congo red binding assay, circular dichroism, light scattering, and atomic force microscopy. We found that the phenolic extract from the peel of apples of the cultivar “Fuji”, cultivated in Sicily (Caltavuturo, Italy), inhibited κ-casein fibril formation in a dose-dependent way. In particular, we found that the extract significantly reduced the protein aggregation rate and inhibited the secondary structure reorganization that accompanies κ-casein amyloid formation. Protein-aggregated species resulting from the incubation of κ-casein in the presence of polyphenols under amyloid aggregation conditions were reduced in number and different in morphology.

Thioflavin

Congo red

Amyloid (mycology)

10.3390/molecules26082371

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Citations (4)

Water Extract of Cryphaea heteromalla (Hedw.) D. Mohr Bryophyte as a Natural Powerful Source of Biologically Active Compounds

International Journal of Molecular Sciences (2019)

Fiorenza Provenzano Jesús Lozano‐Sánchez Estella Rao Radha Santonocito Lorena Anna Ditta

Bryophytes comprise of the mosses, liverworts, and hornworts. Cryphaea heteromalla, (Hedw.) D. Mohr, is a non-vascular lower plant belonging to mosses group. To the date, the most chemically characterized species belong to the liverworts, while only 3.2% and 8.8% of the species belonging to the mosses and hornworts, respectively, have been investigated. In this work, we present Folin-Ciocalteu and oxygen radical absorbance capacity (ORAC) data related to crude extracts of C. heteromalla obtained by three different extraction solvents: pure water (WT), methanol:water (80:20 v/v) (MET), and ethanol:water (80:20 v/v) (ETH). The water extract proved to be the best solvent showing the highest content of biophenols and the highest ORAC value. The C. heteromalla-WT extract was investigated by HPLC-TOF/MS (High Performance Liquid Chromatography-Time of Flight/Mass Spectrometry) allowing for the detection of 14 compounds, five of which were phenolic compounds, derivatives of benzoic, caffeic, and coumaric acids. Moreover, the C. heteromalla WT extract showed a protective effect against reactive oxygen species (ROS) generation induced by tert-butyl hydroperoxide (TBH) on the murine NIH-3T3 fibroblast cell line.

Absorbance

Bryophyte

10.3390/ijms20225560

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Citations (11)

Heat shock protein-27 protects human bronchial epithelial cells against oxidative stress–mediated apoptosis: possible implication in asthma

Cell Stress and Chaperones (2002)

Anna Maria Merendino Catherine J. Morrison Paul Antonio M. Vignola Maria Assunta Costa Mario Melis

Hsp27

10.1379/1466-1268(2002)007<0269:hspphb>2.0.co;2

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Citations (57)