Han Gao

Nanjing Normal University

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i-10 index

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Bin Li

Nanjing Normal University

Xiaoxuan Tian

Tianjin University of Traditional Chinese Medicine

Xiaolei Yu

South China Sea Institute Of Oceanology

Wei Tan

Central South University

Huanyu Zhang

Harbin Medical University

Weiling Jiang

Tianjin International Joint Academy of Biomedicine

Shilin Chen

Chinese Academy of Medical Sciences & Peking Union Medical College

Qinggang Yin

Chinese Academy of Medical Sciences & Peking Union Medical College

Jing Tang

Sichuan University

Yanxiao Li

Affiliated Eye Hospital of Wenzhou Medical College

Cooperative Institutions

Chinese Academy of Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Nanjing Normal University

University of Chinese Academy of Sciences

Shanghai Jiao Tong University

Sun Yat-sen University

Chinese Academy of Agricultural Sciences

Peking University

China Academy of Chinese Medical Sciences

Tianjin University of Traditional Chinese Medicine

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Genome-Wide Analysis of Light-Regulated Alternative Splicing in Artemisia annua L.

Frontiers in Plant Science (2021)

Tingyu Ma Han Gao Dong Zhang Wei Sun Qinggang Yin

Artemisinin is currently the most effective ingredient in the treatment of malaria, which is thus of great significance to study the genetic regulation of Artemisia annua . Alternative splicing (AS) is a regulatory process that increases the complexity of transcriptome and proteome. The most common mechanism of alternative splicing (AS) in plant is intron retention (IR). However, little is known about whether the IR isoforms produced by light play roles in regulating biosynthetic pathways. In this work we would explore how the level of AS in A. annua responds to light regulation. We obtained a new dataset of AS by analyzing full-length transcripts using both Illumina- and single molecule real-time (SMRT)-based RNA-seq as well as analyzing AS on various tissues. A total of 5,854 IR isoforms were identified, with IR accounting for the highest proportion (48.48%), affirming that IR is the most common mechanism of AS. We found that the number of up-regulated IR isoforms (1534/1378, blue and red light, respectively) was more than twice that of down-regulated (636/682) after treatment of blue or red light. In the artemisinin biosynthetic pathway, 10 genes produced 16 differentially expressed IR isoforms. This work demonstrated that the differential expression of IR isoforms induced by light has the potential to regulate sesquiterpenoid biosynthesis. This study also provides high accuracy full-length transcripts, which can be a valuable genetic resource for further research of A. annua , including areas of development, breeding, and biosynthesis of active compounds.

Artemisia annua

RNA-Seq

Metabolic pathway

Proteome

10.3389/fpls.2021.733505

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Citations (6)

Characterization and expression profiling of G protein-coupled receptors (GPCRs) in Spodoptera litura (Lepidoptera: Noctuidae)

Comparative Biochemistry and Physiology Part D Genomics and Proteomics (2022)

Yanxiao Li Han Gao Hui Zhang Runnan Yu Fan Feng

Spodoptera litura

Subfamily

10.1016/j.cbd.2022.101018

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Citations (6)

B/N-Doped p-Arylenevinylene Chromophores: Synthesis, Properties, and Microcrystal Electron Crystallographic Study

Journal of the American Chemical Society (2020)

Hua Lü Takayuki Nakamuro Keitaro Yamashita Haruaki Yanagisawa Osamu Nureki

Linearly conjugated systems have long served as an archetype of conjugated materials, but suffer from two intrinsic structural problems: potential instability due to intermolecular interactions and the flexibility of the C–C bonds connecting C═C bonds. Efforts to solve these problems have included the insertion of aromatic units as a part of the conjugation and the introduction of carbon bridges to stop the bond rotation. We report here B/N-doped p-arylenevinylene chromophores synthesized through the incorporation of a cyclopenta[c][1,2]azaborole framework as a part of the conjugated system. The ring strain intrinsic to this new skeleton both flattens and rigidifies the conjugation, and the B––N+ dative bond is much easier to form than a C–C bond, which simplifies the synthetic design. The B–N dative bond also reduces the HOMO–LUMO gap, thereby causing a significant redshift of the absorption and emission compared with their all-carbon congeners while retaining high photostability and high fluorescence quantum yield in both solution and film states. A doubly B/N-doped compound showed emission peaks at 540 nm with a small Stokes shift of 20 nm and a fluorescence quantum yield of 98%. The molecules serve as excellent lipophilic fluorescent dyes for live-cell imaging, showing a higher photostability than that of commercially available BODIPY-based dyes.

Chromophore

Quantum yield

HOMO/LUMO

Single bond

10.1021/jacs.0c10337

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Citations (42)

Development of a Specific Mini-Barcode From Plastome and its Application for Qualitative and Quantitative Identification of Processed Herbal Products Using DNA Metabarcoding Technique: A Case Study on Senna

Frontiers in Pharmacology (2020)

Xiaolei Yu Wei Tan Han Gao Lin Miao Xiaoxuan Tian

Herbal products play an important role globally in the pharmaceutical and healthcare industries. However, some specific groups of herbal products are easily adulterated by confused materials on the market, which seriously reduces the products’ quality. Universal conventional DNA barcodes would function poorly since the processed herbal products generally suffer from varying degrees of DNA degradation and DNA mixing during processing or manufacturing. For quality control purposes, an accurate and effective method should be provided for species identification of these herbal products. Here, we provided a strategy of developing the specific mini-barcode using Senna as an example, and by coupling with the metabarcoding technique, it realized the qualitative and quantitative identification of processed herbal products. The plastomes of Senna obtusifolia (L.) H.S.Irwin & Barneby and Senna occidentalis (L.) Link were newly assembled, and the hypervariable coding-regions were identified by comparing their genomes. Then, the specific mini-barcodes were developed based on the identified hypervariable regions. Finally, we applied the DNA metabarcoding technique to the developed mini-barcodes. Results showed that the lengths of plastomes of S. obtusifolia and S. occidentalis were 162,426 and 159,993 bp, respectively. Four hypervariable coding-regions ycf1 , rpl23 , petL , and matK were identified. Two specific mini-barcodes were successfully developed from matK , and the mini-barcode of primer 647F-847R was proved to be able to qualitatively and quantitatively identify these two processed Senna seeds. Overall, our study established a valuable way to develop the specific mini-barcode, which may provide a new idea for the quality control of processed herbal products.

Barcode

DNA Barcoding

Hypervariable region

Identification

10.3389/fphar.2020.585687

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Citations (20)

Species‐specific duplicated FMRFaR‐like gene A62 regulates spontaneous locomotion in Apolygus lucorum

Pest Management Science (2024)

Han Gao Ying Tian Hui Zhang Yanxiao Li Chenjun Li

Abstract BACKGROUND Apolygus lucorum , a major cotton pest, has undergone a significant expansion of the FMRFaR gene within the GPCR superfamily, resulting in two classes of GPCR, namely FMRFaR (A54‐55) and newly duplicated FMRFaR‐like (A56‐62). Notably, FMRFaR‐like genes, particularly A62, show enhanced expression in the legs and wings of adults, indicating their potential role in locomotion. Employing A62 as a representative of FMRFaR‐like, our study investigates the influence of FMRFa, FMRFaR, and FMRFaR‐like on locomotion and development of A. lucorum . RESULTS FMRFaR and FMRFa exhibit comparable temporal and tissue expression patterns, whereas the FMRFaR‐like genes within A. lucorum exhibit completely distinct evolutionary and expression patterns compared to classical FMRFaR. RNA interference (RNAi) experiments revealed that suppressing FMRFa expression results in complete lethality in A. lucorum , but neither FMRFaR nor A62 exhibit the same effect after RNAi. Suppressing the expression of FMRFa only decreases the expression of the A54 gene simultaneously, suggesting that A54 may function as a classical FMRFaR activated by FMRFa. RNAi of A62 leads to wing malformation and a significant reduction in spontaneous movement behavior in A. lucorum . Further transcriptomic analysis revealed that A62 affects the A. lucorum 's movement behavior through energy metabolism pathways and motor protein pathways. CONCLUSION Our study unveils the unique and complex roles of FMRFa and its receptor in A. lucorum . These findings provide valuable insights into potential targets for pest control strategies aimed at managing A. lucorum populations in cotton fields. © 2024 Society of Chemical Industry.

10.1002/ps.8039

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Citations (0)

Synthesis of dabigatran etexilate

Xiandai yaowu yu linchuang (2014)

Han Gao

Objective To study the synthetic technology of dabigatran etexilate. Methods 3-[[[2-[[(4-Cyanophenyl)amino]methyl]-1-methyl-1H-benzimidazol-5-yl]carbonyl] pyridin-2- ylamino]propionic acid ethyl ester was used as starting material to synthesize amidine by Pinner reaction, then to obtain target compound by reacting with hexyl chloroformate. Results The target compound was synthesized and characterized by MS and 1H-NMR. The yield was 38.8% and the purity was 99.6%. Conclusion The synthetic route of dabigatran etexilate has the advantages of simple operation and reasonable design with high yield and purity, and is suitable for industrial production.

Amidine

Ethyl chloroformate

Source

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Tectorigenin monohydrate: an isoflavone fromBelamcanda chinensis

Acta Crystallographica Section E Structure Reports Online (2008)

Benguo Liu Yuxiang Ma Han Gao Qiong Wu

THE TITLE COMPOUND [SYSTEMATIC NAME: 5,7-dihydr-oxy-3-(4-hydroxy-phen-yl)-6-meth-oxy-4H-chromen-4-one monohydrate], C(16)H(12)O(6)·H(2)O, is isolated from Belamcanda chinensis and is said to have anti-microbiotic and anti-inflammatory effects. The chromen-4-one system and the benzene ring are inclined at a dihedral angle of 36.79 (6)°. Molecules are linked by inter- and intramolecular O-H⋯O hydrogen bonds.

Dihedral angle

10.1107/s1600536808030833

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Citations (2)

[Effects of anti-heparanase antibody on the growth and invasion of HCCLM6 human hepatocellular carcinoma cells].

PubMed (2009)

Xiao-mei Han Hui‐Ju Wang Jian-Min Yang Yonglie Zhou Hou‐Quan Tao

To investigate the effects of the self-developed anti-heparanase polypeptide antibodies on growth and invasion of human hepatocellular carcinoma HCCLM6 cells.Using MTT, flow cytometry, plate clone formation, transwell invasion and heparan degrading enzyme assay, the growth and invasion changes of human hepatocellular carcinoma HCCLM6 cells by co-culture with each of three self-developed rabbit anti-heparanase polyclonal antibodies were detected.Compared with normal rabbit IgG, in the presence of each anti-heparanase polypeptide antibody, the growth, cell cycle and clone formation remained unchanged, and under the P1 or P2 anti-heparanase polypeptide antibody (with final concentration 100 microg/ml), the cell invasiveness was inhibited by 52.5% and 36.6%, respectively, and the heparanase activity was inhibited by 42.9% and 39.1%, respectively.The P1 and P2 anti-heparanase polypeptide antibodies can effectively inhibit the invasion ability and heparanase activity of liver cancer HCCLM6 cells. However, All the three antibodies have no effects on its growth, cell cycle and clone formation.

Heparanase

Polyclonal antibodies

clone (Java method)

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Citations (5)

Insight into role of short chain fatty acids in regulating intestinal mucosal barrier and alleviating inflammatory bowel disease

Shijie huaren xiaohua zazhi (2022)

Xue-Ran Guo Chengwei He Han Gao Rongxuan Hua Liang Chen

10.11569/wcjd.v30.i21.928

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Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

Frontiers in Endocrinology (2021)

Han Gao Yanxiao Li Miao Wang Xiaowen Song Jing Tang

G protein-coupled receptors (GPCRs) are the largest and most versatile family of transmembrane receptors in the cell and they play a vital role in the regulation of multiple physiological processes. The family Miridae (Hemiptera: Heteroptera) is one of the most diverse families of insects. Until now, information on GPCRs has been lacking in Miridae. Apolygus lucorum , a representative species of the Miridae, is an omnivorous pest that occurs worldwide and is notorious for causing serious damage to various crops and substantial economic losses. By searching the genome, 133 GPCRs were identified in A. lucorum . Compared with other model insects, we have observed GPCR genes to be remarkably expanded in A. lucorum , especially focusing on biogenic amine receptors and neuropeptide receptors. Among these, there is a novel large clade duplicated from known FMRFamide receptors (FMRFaRs). Moreover, the temporal and spatial expression profiles of the 133 genes across developmental stages were determined by transcriptome analysis. Most GPCR genes showed a low expression level in the whole organism of A. lucorum . However, there were a few highly expressed GPCR genes. The highly expressed LW opsins in the head probably relate to nocturning of A. lucorum , and the expression of Cirl at different times and in different tissues indicated it may be involved in growth and development of A. lucorum . We also found C2 leucine-rich repeat-containing GPCRs (LGRs) were mainly distributed in Hemiptera and Phthiraptera among insects. Our study was the first investigation on GPCRs in A. lucorum and it provided a molecular target for the regulation and control of Miridae pests.

10.3389/fendo.2021.773669

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Citations (16)