EB Mechetner

Atotech (United States)

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Roninson Ib

Novartis (Japan)

PM Chaudhary

University of Illinois Chicago

ЕС

Е. С. Иевлева

Belgorod State Technological University

Jayne Gilbert

Calvary Mater Newcastle Hospital

Susan L. Cohn

University of Chicago

S B Bordow

Sydney Children's Hospital

M Holló

NTL Institute for Applied Behavioral Science

Balázs Sarkadi

HUN-REN Research Centre for Natural Sciences

Janice Madafiglio

Children's Cancer Institute Australia

GM Marshall

Sydney Children's Hospital

Cooperative Institutions

Sydney Children's Hospital

Children's Cancer Institute Australia

University of Illinois Chicago

UNSW Sydney

Lurie Children's Hospital

Cancer Institute of New South Wales

Ministry of Health of the Russian Federation

Russian Cancer Research Center NN Blokhin

University of Sydney

University of Chicago

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Selective cytotoxic effect of immunotoxin on mouse erythroid tumor cells

A. G. Tonevitskii EB Mechetner Emilia Rozinova Е. С. Иевлева

At the present time, significant progress has been made in creating conjugates of antibodies and toxins (or their catalytic subunits). Such hybrid molecules, immunotoxins (IT), are highly specific cytotoxic agents. The authors investigate the possibility of selective elimination of tumor cells. The cytotoxic properties of the IT, its component parts, and a control conjugate were determined according to effectiveness of inhibition of /sup 14/C-leucine incorporation into protein cells being synthesized. The control conjugate (non-immune rat IgG-R/sub a/) was derived in the same way as the IT. Holes of a 96-hole plate (Linbro) were filled with 50,000 cells in 50 microliters of MEM medium without leucine (FLOW) with 10% fetal serum (FLOW). A test sample, 50 microliters, was added to cells and incubated 30 min at 37/sup 0/C, 5% CO/sub 2/. Then cells were washed twice, 200 microliters of medium without leucine were added, and preparations were left for 16 h at 37/sup 0/C, 5% CO/sub 2/. If cells were treated sequentially with two preparations, the second preparation was added after washing and incubated as described above. After 16 h of incubation, /sup 14/C-leucine (UVVVR) was added for 3 h to a final concentration of 1.3 micro-Ci/ml. Cells were collected usingmore » a Cell Harvester (FLOW) on G F/C glass filters. Filters were dried and read on a scintillation counter.« less

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L1210 cells

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A new method for quantitative assessment of P-glycoprotein-related multidrug resistance in tumour cells

British Journal of Cancer (1996)

László Homolya M Holló Michael Müller EB Mechetner Balázs Sarkadi

Calcein

P-glycoprotein

10.1038/bjc.1996.151

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