Abstract Summary: Material coating of surfaces can enhance receptivity for cells and biological compounds. Existing plasma coating technologies and possible materials are limited. A new polymer from pentafluorophenyl methacrylate (PFM) monomer was synthesized, and was plasma enhanced chemical vapor deposited on silicon wafers. The optimal plasma polymerization parameters for the PFM monomer and its copolymerization with the cross‐linking agents 1,7‐octadiene and 1,4‐butanediol divinyl ether co‐monomers were established. All the resulting polymer coatings leave the labile pentafluorophenyl group on the surface, enabling a rapid reaction with an amino‐terminated biotin ligand and allowing layer‐by‐layer self‐assembly of biotin‐streptavidin. In addition, the deposited polymer layers showed an extremely flat morphology with a nanoscale average roughness. This approach provides an easy means of obtaining functionalized surfaces which can enhance and control the biocompatibility of bulk materials. Merging the versatility of plasma polymerization processes, via simple monomers and reaction conditions, with biological platforms that enable target of cell adhesion brings us closer to the ultimate goal of controlling cell function through structured surfaces for their application in tissue engineering. Schematic representation of a functionalized surface obtained by plasma polymerization of the PFM monomer, showing labile pentafluorophenyl groups on the surface. magnified image Schematic representation of a functionalized surface obtained by plasma polymerization of the PFM monomer, showing labile pentafluorophenyl groups on the surface.

Plasma polymerization

Surface Modification

Streptavidin

10.1002/ppap.200500042

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Citations (41)

Surface Reactivity of Pulsed-Plasma Polymerized Pentafluorophenyl Methacrylate (PFM) toward Amines and Proteins in Solution

Langmuir (2007)

Laia Francesch Salvador Borrós Wolfgang Knoll Renate Förch

Pulsed-plasma polymerization has been used to deposit ultrathin layers of pentafluorophenyl methacrylate by using low duty cycles and low power input. The monomer structure can be retained such that the chemical reactivity of the active ester group could be studied using the reaction with a simple amine. The film properties in aqueous phosphate buffer have been investigated using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and real time surface plasmon resonance spectroscopy. The films react readily with diaminohexane and immunoglobulin (IgG), yet the reactivity shows a dependence on the extent of hydrolysis of the ester group.

Reactivity

10.1021/la062422d

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Citations (42)

APC Targeting: mRNA Delivery System for Targeting Antigen‐Presenting Cells In Vivo (Adv. Healthcare Mater. 17/2018)

Advanced Healthcare Materials (2018)

Cristina Fornaguera Marta Guerra‐Rebollo Miguel Ángel Lázaro Cristina Castells‐Sala Óscar Meca‐Cortés

The study in article 1800335 by Salvador Borrós and co-workers, takes advantage of the known versatile properties of the oligopeptide end-modified poly (β-amino esters) (OM-PBAEs) to complex mRNA and form nanoparticles. It is demonstrated that the selection of the appropriate end-oligopeptide modifications enables the specific targeting and major transfection of antigen-presenting cells (APC) in vivo, after intravenous administered.

Oligopeptide

10.1002/adhm.201870071

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Citations (1)

Conducting Plasma Polymerized Polypyrrole Thin Films as Carbon Dioxide Gas Sensors

Plasma Processes and Polymers (2012)

José Luis Yagüe Salvador Borrós

Abstract This paper describes the production of conducting polypyrrole (PPy) thin films by plasma‐enhanced chemical vapor deposition (PECVD) and its posterior use for sensing applications of carbon dioxide (CO 2 ). Since PECVD yields the non‐conducting form of PPy, a doping process consisting of exposure to iodine vapor is required to obtain electrically conducting samples. Differences in the chemical state and topography between the doped and undoped systems are studied and characterized to demonstrate the doping effect on the polymer. Furthermore, changes in conductivity after exposure to CO 2 gas are measured to evaluate the use of these thin films as chemical sensors. The results suggest that plasma polymerized films show a good sensitivity and reproducibility in the analyte detection. magnified image

Polypyrrole

Plasma polymerization

10.1002/ppap.201100169

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Citations (27)

Engineering oncogene-targeted anisamide-functionalized pBAE nanoparticles as efficient lung cancer antisense therapies

RSC Advances (2023)

Cristina Fornaguera Antoni Torres-Coll Laura Olmo Coral García-Fernández Marta Guerra‐Rebollo

Non-small cell lung cancer (NSCLC) is one of the leading causes of worldwide death, mainly due to the lack of efficient and safe therapies. Currently, NSCLC standard of care for consist on the use of traditional chemotherapeutics, non-selectively distributed through the whole body, thus causing severe side effects while not achieving high efficacy outcomes. Consequently, the need of novel therapies, targeted to modify specific subcellular routes aberrantly expressed only in tumor cells is still urgent. In this context, the delivery of siRNAs that can know-down overexpressed oncogenes, such as mTOR, could become the promised targeted therapy. However, siRNA effective delivery remains a challenge due to its compromised stability in biological fluids and its inability to cross biological and plasmatic membranes. Therefore, polymeric nanoparticles that efficiently encapsulate siRNAs and are selectively targeted to tumor cells could play a pivotal role. Accordingly, we demonstrate in this work that oligopeptide end-modified poly(beta aminoester) (OM-pBAE) polymers can efficiently complex siRNA in small nanometric particles using very low polymer amounts, protecting siRNA from nucleases attack. These nanoparticles are stable in the presence of serum, advantageous fact in terms of in vivo use. We also demonstrated that they efficiently transfect cells in vitro, in the presence of serum and are able to knock down target gene expression. Moreover, we demonstrated their antitumor efficacy by encapsulating mTOR siRNA, as a model antisense therapy, which showed specific lung tumor cell growth inhibition in vitro and in vivo. Finally, through the addition of anisamide functionalization to the surface of the nanoparticles, we proved that they become selective to lung tumor cells, while not affecting healthy cells. Therefore, our results are a first step in the discovery of a tumor cell-targeted efficient silencing nanotherapy for NSCLC patients survival improvement.

Targeted Therapy

10.1039/d3ra05830a

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ZINC OXIDE VERSUS MAGNESIUM OXIDE REVISITED. PART 1

Rubber Chemistry and Technology (2012)

Manuel Guzmán Berta Vega Núria Agulló Ulrich Giese Salvador Borrós

Abstract Zinc oxide is a widely used compound in the rubber industry due to the excellent properties that it shows as activator, and consequently, its role in the mechanism of accelerated sulfur vulcanization has been extensively studied. Due to the increased concern about its environmental effects, several research studies have been carried out in order to substitute it with different metal oxides such us MgO. The effect of the activator system in order to minimize the environmental impact of the rubber goods has been explored. The work developed is presented in two parts. In Part 1, the influence of different mixtures of ZnO and MgO on the vulcanization of natural rubber has been investigated. In Part 2, model compound vulcanization has been used to study the role of MgO on the mechanism to gain a better understanding of the differences shown in Part 1.

10.5254/1.3672428

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Citations (24)