Hans Hoek

Radboud University Nijmegen

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Pilar Laguna

Istanbul Medipol University

Coen Schoots

Radboud University Nijmegen

Jean de la Rosette

Istanbul Medipol University

Frank Smedts

Cork University Hospital

Cooperative Institutions

Istanbul Medipol University

Kasturba Medical College, Manipal

Kasturba Medical College Hospital

University of Amsterdam

Hôpital Tenon

Sorbonne Université

Chinese University of Hong Kong

Men's Health Boston

Bio-Medical Science (South Korea)

Clarke (United States)

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Changing Patterns of Keratin Expression could be Associated with Functional Maturation of the Developing Human Bladder

The Journal of Urology (2002)

Jean de la Rosette Frank Smedts Coen Schoots Hans Hoek Pilar Laguna

We investigate the keratin phenotype of human transitional epithelium at various gestational ages and whether keratin composition of transitional epithelium is related to bladder function and morphology.Consecutive sections from formalin fixed paraffin embedded blocks of autopsy bladder tissue from 21 male and 5 female fetuses, gestational age 12 to 40 weeks and 7 infants 2 days to 19 months old were cut and stained with antibodies recognizing basal cell keratins 5, 14 and 17, intermediate squamous cell keratin 13 and columnar cell keratins 7, 8, 18 and 20.With gestational age there were distinct changes in expression of keratins recognizing columnar cells, consisting of focal loss of keratin 7 in transitional epithelium, restriction of keratin 20 expression to umbrella cells and expression of keratin 18 throughout the full thickness of transitional epithelium. Basal cell keratin 5 was found above the basal cell layer while keratins 14 and 17 were not found. Squamous cell keratin 13 was found throughout the full thickness of the urothelium.The changes with gestational age in expression of some keratins may be related to the development of the reservoir function of the bladder. The impermeability of transitional epithelium, particularly during early fetal development, is possibly a function of umbrella and intermediate transitional cells.

Keratin 5

Keratin 8

Keratin 7

Keratin 6A

Keratin 14

Transitional Cell

Cell type

Basal (medicine)

Urothelial Cell

10.1097/00005392-200208000-00085

Cite

Citations (5)

Changing Patterns of Keratin Expression could be Associated with Functional Maturation of the Developing Human Bladder

The Journal of Urology (2002)

Jean de la Rosette Frank Smedts Coen Schoots Hans Hoek Pilar Laguna

No AccessJournal of UrologyPEDIATRIC UROLOGY: Classic Article in Pediatric Urology1 Aug 2002Changing Patterns of Keratin Expression could be Associated with Functional Maturation of the Developing Human Bladder Jean de la rosette, Frank Smedts, Coen Schoots, Hans Hoek, and Pilar Laguna Jean de la rosetteJean de la rosette , Frank SmedtsFrank Smedts , Coen SchootsCoen Schoots , Hans HoekHans Hoek , and Pilar LagunaPilar Laguna View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64731-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We investigate the keratin phenotype of human transitional epithelium at various gestational ages and whether keratin composition of transitional epithelium is related to bladder function and morphology. Materials and Methods: Consecutive sections from formalin fixed paraffin embedded blocks of autopsy bladder tissue from 21 male and 5 female fetuses, gestational age 12 to 40 weeks and 7 infants 2 days to 19 months old were cut and stained with antibodies recognizing basal cell keratins 5, 14 and 17, intermediate squamous cell keratin 13 and columnar cell keratins 7, 8, 18 and 20. Results: With gestational age there were distinct changes in expression of keratins recognizing columnar cells, consisting of focal loss of keratin 7 in transitional epithelium, restriction of keratin 20 expression to umbrella cells and expression of keratin 18 throughout the full thickness of transitional epithelium. Basal cell keratin 5 was found above the basal cell layer while keratins 14 and 17 were not found. Squamous cell keratin 13 was found throughout the full thickness of the urothelium. Conclusions: The changes with gestational age in expression of some keratins may be related to the development of the reservoir function of the bladder. The impermeability of transitional epithelium, particularly during early fetal development, is possibly a function of umbrella and intermediate transitional cells. References 1 : The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell1982; 31: 11. Crossref, Medline, Google Scholar 2 : Cytokeratine 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol1992; 140: 427. Google Scholar 3 : Keratins as differentiation markers in tumor biology and surgical pathology. In: Current Perspectives on Molecular and Cellular Oncology. Edited by . London: JAI Press1992: 285. Google Scholar 4 : Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract. Histochemistry1989; 91: 151. Google Scholar 5 : Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines. Am J Pathol1988; 132: 123. Google Scholar 6 : Expression of cytokeratin 20 redefines urothelial papillomas of the bladder. Lancet1999; 353: 974. Google Scholar 7 : Cytokeratin 20: a new marker for early detection of bladder cell carcinoma?. J Urol1998; 160: 1971. Link, Google Scholar 8 : Expression of intermediate filament proteins in fetal and adult human kidney: modulations of intermediate filament patterns during development and in damaged tissue. Lab Invest1991; 65: 74. Google Scholar 9 : Keratin immunohistochemistry in normal human liver. Cytokeratin pattern of hepatocytes, bile ducts and acinar gradient. Virchows Arch A Pathol Anat Histopathol1987; 412: 63. Google Scholar 10 : Identification of intermediate cell types by keratin expression in the maturing human prostate. Prostate1998; 34: 292. Google Scholar 11 Kortmann, B. B. M., Holm, N. R., Smedts, F. et al: Bladder outlet obstruction does not change bladder wall morphology. Unpublished data. Google Scholar 12 : Role of mesenchymal-epithelial interactions in normal bladder development. J Urol1996; 156: 1820. Link, Google Scholar 13 : Mesenchymal-epithelial interactions during androgen-induced development of the prostate. Prog Clin Biol Res1985; 171: 15. Google Scholar 14 : Ontogeny of the rat bladder: smooth muscle and epithelial differentiation. Acta Anat (Basel)1996; 155: 163. Google Scholar 15 : Measurement of fetal urine production in normal pregnancy by real time ultrasonography. Am J Obstet Gynecol1989; 161: 1264. Google Scholar 16 : Bovine bladder compliance increases with normal fetal development. J Urol1994; 152: 692. Link, Google Scholar 17 : The fine structure of the human fetal urinary bladder. Development and maturation. A light, transmission and scanning electron microscopic study. J Anat1989; 166: 135. Google Scholar 18 : Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer etiology. Virchows Arch1994; 425: 145. Google Scholar 19 : Mammalian uroplakins. A group of highly conserved urothelial differentiation-related membrane proteins. J Biol Chem1994; 269: 13716. Crossref, Medline, Google Scholar 20 : The development of the human prostate gland with reference to the development of other structures at the neck of the urinary bladder. Am J Anat1901; 13: 299. Google Scholar 21 : Usefulness of immunoperoxidase staining with high-molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland. Virchows Arch A Pathol Anat Histopathol1990; 417: 191. Google Scholar 22 : Anti-cytokeratin 5/6: a positive marker for epithelioid mesothelioma. Histopathology1997; 31: 140. Google Scholar 23 : Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol1990; 136: 657. Google Scholar From the Department of Urology, University Medical Center, St. Radboud Hospital, Nijmegen and the *Department of Pathology, Foundation of Collaborating Hospitals of Eastern Groningen, Groningen, The Netherlands© 2002 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 168Issue 2August 2002Page: 709-717 Advertisement Copyright & Permissions© 2002 by American Urological Association, Inc.Keywordscell differentiationkeratinembryologyintermediate filamentsMetrics Author Information Jean de la rosette More articles by this author Frank Smedts More articles by this author Coen Schoots More articles by this author Hans Hoek More articles by this author Pilar Laguna More articles by this author Expand All Advertisement PDF downloadLoading ...

Keratin 7

Keratin 5

Keratin 8

Keratin 6A

Transitional Cell

Keratin 14

Basal (medicine)

Rosette (schizont appearance)

10.1016/s0022-5347(05)64731-3

Cite

Citations (18)