This paper examines whether muscle fatigue by functional electrical stimulation (FES) can be estimated from changes in skin impedance. FES is a system that moves a human body by electrical stimulation. Muscle fatigue reduces muscle exerted force, and may cause accidents such as rehabilitation with FES. However, practical researches that can estimate muscle fatigue have not been reported. Therefore, this study focuses on relationship between muscle electrical properties and muscle fatigue or muscle exerted force by FES. Finally, experimental results show that skin impedance and muscle fatigue are uncorrelated.