H. R. Hosseinian Moghaddam

National Institute of Genetics

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Parvin Shariati

National Institute of Genetic Engineering and Biotechnology

Bagher Yakhchali

National Institute of Genetic Engineering and Biotechnology

Mohammad Taghi Hamed Mosavian

Ferdowsi University of Mashhad

Fahimeh Ghasemi

Birjand University of Medical Sciences

Fatemeh Tabandeh

National Institute of Genetic Engineering and Biotechnology

Cooperative Institutions

National Institute of Genetics

National Institute of Genetic Engineering and Biotechnology

Birjand University of Medical Sciences

Tarbiat Modares University

Azarbaijan Shahid Madani University

Centro de Ingeniería Genética y Biotecnología

Ferdowsi University of Mashhad

Faculty (United Kingdom)

Engineering (Italy)

Islamic Azad University, Mashhad

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

FED-BATCH FERMENTATION OFBACILLUS CLAUSIIFOR EFFICIENT PRODUCTION OF ALKALINE PROTEASE USING DIFFERENT FEEDING STRATEGIES

Chemical Engineering Communications (2011)

Fatemeh Tabandeh H. R. Hosseinian Moghaddam Bagher Yakhchali Parvin Shariati Mohammad Taghi Hamed Mosavian

The effect of different feeding strategies, involving constant rate and linear feeding with negative and positive slopes, on protease production of an indigenous Bacillus clausii was investigated. The results indicated that alkaline protease was produced at high levels soon after glucose was completely consumed. Alkaline protease activity was at a maximum during the constant feeding rate and in the absence of glucose and presence of mineral salts and yeast extract in the feed medium. Maximum protease production in the fed-batch culture using an optimized level of feeding composition was 2430 ± 67 U/mL, which increased by up to 35% when compared to the 1800 ± 14 U/mL produced during batch culture. During batch fermentation, the protease yield and productivity obtained were 90000 U/g and 64285 U/L · h; however, under fed-batch conditions, these were 121500 U/g and 71470 /L · h, respectively. Hence, the suggested strategy has the potential to be applied to industrial production of protease used in detergent products.

Yeast extract

Alkaline protease

Fed-batch culture

10.1080/00986445.2011.552024

Cite

Citations (7)