A system for observing bladder cancer cells in form of a miniaturized fluorescence microscope has been developed. Attention has turned to integrate this system in an existing LTCC (low temperature cofired ceramics) module. Software with a region detecting algorithm has been written to automatically detect the captured images. The cancer specimen was marked with hypericin, which is selective on bladder cancer cells. After coloring the specimen was excited with green light of a high power LED. The red emitting hypericin marked cells were captured by a CMOS chip and evaluated with the software to confirm or negate a suspicion of cancer.
Based on flow models applied in former analyses, three dimensional numerical flow simulations were performed for a spherical reactor cell of a biological monitoring module with respect to different inflow configurations and respective mass flow rates. Time — dependent simulations were performed for the case of dyed water and clear water flowing through different inlets at varying mass flow rates into the spherical cavity of reactor cell and channel system which connects the relevant measuring ports of the monitoring module. Spectroscopic analyses of light absorption at a port have been carried out and the results compared with the local concentration distribution predicted for this port by means of FE - analyses (Finite Element) using the CFD (Computational Fluid Dynamics) — program package of Fluent Inc.
Abstract Abstract In this review article, the diagnostic applications of fluorescence spectroscopy (FS) in subspecialties of medical sciences are reviewed. The main purpose of this article is to present a critical report on various diagnostic applications of FS. Fluorescence spectroscopy is an emerging excellent diagnostic tool for many diseases, especially in the diagnosis of early stage cancers. Fluorescence spectroscopy is shown to be a more sensitive and rapid diagnostic tool with high efficiency compared to many routine medical diagnostic tools. However, there is still a great need for clinical trials and studies on a large scale to establish the validity of this new diagnostic technique. There is a need to highlight this issue among the scientific community. Current research, available instrumentation, and proposed areas that should be the focus of future research are presented and discussed in this review article. Keywords: Fluorescence spectroscopymodern diagnostic toolsfluorescence correlation spectroscopymedical sciences Acknowledgment The authors acknowledge "The Vienna Science and Technology Fund" (WWTF), Vienna, Austria, for the generous funding of Mathematics and Biology call 2007 project.
Abstract Cancer is one of the big killers of world population. The majority of cancers are diagnosed at a late stage, making a cure almost impossible. Fluorescence spectroscopy is an emerging diagnostic tool for various medical diseases including premalignant and malignant lesions. Fluorescence spectroscopy is a noninvasive technique and has been applied successfully for the diagnosis of multisystem cancers with high sensitivity and specificity. Fluorescence spectroscopy minimizes the need for repetitive biopsy, which is routine practice for cancer patient follow-up. But there are many aspects of this new diagnostic technique that should be discussed in future research to overcome limitations and challenges faced by this technique for diagnosis of cancers.
