J Pawinwongchai

Rangsit University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Supantitra Chanprasert

Chulalongkorn University

Y Suwanawong

Viroj Wiwanitkit

Saveetha University

Suphan Soogarun

Chulalongkorn University

Paweena Pradniwat

Chulalongkorn University

Tusrin Moungkote

Chulalongkorn University

Montri Chowbumroongkait

Thai Red Cross Society

Cooperative Institutions

Chulalongkorn University

University of Health and Allied Sciences

Saveetha University

Dr. D. Y. Patil Medical College, Hospital and Research Centre

Chandigarh University

Dr. D.Y. Patil Vidyapeeth, Pune

University of Nis

Joseph Ayo Babalola University

Bangkok Hospital

D.Y. Patil University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Induction of apoptosis and cytokines secretion of Houttuynia cordata Thunb. flavonoids in human leukemic cells

Planta Medica (2014)

Supantitra Chanprasert J Pawinwongchai

Leukaemia is a hematopoietic cancer and the treatment has many side effects. For this reason, herbal therapy is an alternative treatment for leukaemia to reduce the side effects. The medicinal herb Plucao (Houttuynia cordata Thunb.) has a vary biological activities including anti-cancer, anti-leukemic, anti-microbial, anti-oxidant, anti-inflammatory and immunomodulatory effects. The active components in Plucao are alkaloids and flavonoids. Quercetin, quercitrin and hyperoside are flavonoid and flavonoid glycosides founded in Plucao. In this study, we extracted Plucao by both distilled deionized water and ethanol. We found that H.cordata ethanolic extract had total flavonoid content more than H.cordata water extract. Total flavonoid of H.cordata ethanolic extract was 224.90 ± 2.80 mg Quercitrin Equivalent (QE)/g dried H.cordata while total flavonoid of H.cordata water extract was 130.79 ± 4.89 mg QE/g dried H.cordata. Moreover, H.cordata extract, quercetin, quercitrin and hyperoside did inhibit the proliferation of Jurkat and U937 leukemic cell lines. The half maximal inhibitory concentration (IC50) values of H.cordata ethanolic extract, H.cordata water extract quercetin, quercitrin and hyperoside on Jurkat were 403.30 ± 5.35, 445.10 ± 6.49, 68.50 ± 2.86, 73.10 ± 2.59, and 75.60 ± 2.25 mg/ml, respectively while IC50 values of H.cordata ethanolic extract, H.cordata water extract quercetin, quercitrin and hyperoside on U937 were 386.20 ± 5.28, 416.40 ± 6.78, 60.50 ± 2.95, 70.10 ± 3.01 and 75.10 ± 1.77 mg/ml, respectively. In addition, our cytokines study found that H.cordata extract and flavonoids especially H.cordata ethanolic extract and quercetin substantially reduced the hematopoietic (IL-2, G-CSF and GM-CSF) and inflammatory (IL-6, IL-10, IL-13, IL-17, IFN-γ, MIP-1β, TNF-α) cytokines. Therefore, we conclude that H.cordata has anti-leukemic and anti-inflammatory activities and may be a potential herbal treatment for leukaemia in complementary medicine.

Quercitrin

Hyperoside

Houttuynia cordata

10.1055/s-0034-1394631

Cite

Citations (0)

Effect of Trimeresurus albolabris (green pit viper) venom on mean corpuscular volume, osmotic fragility and red blood cell morphology: A preliminary report

AFRICAN JOURNAL OF BIOTECHNOLOGY (2007)

Suphan Soogarun Montri Chowbumroongkait Paweena Pradniwat Supantitra Chanprasert Viroj Wiwanitkit

An in vitro study was conducted by mixing small amounts of green pit viper venom with blood and observing changes. At a concentration of 10 mg crude venom, red blood cells (RBC) osmotic fragility slightly increased. RBC morphology changed to spherical shape which was compatible with what was observed in scanning electron microscope (SEM). However, there was no change in mean corpuscular volume (p > 0.05).

VIPeR

Erythrocyte fragility

Morphology

10.5897/ajb2007.000-2154

Cite

Citations (3)