Forty-nine strains belonging to the genus Gluconobacter were re-examined with respect to their species identification based on the sequences of the 16S rDNA and 16S-23S rDNA internal transcribed spacer regions (ITS). A phylogenetic tree constructed from the 16S rDNA sequences indicated the presence of five clusters corresponding, respectively, to the major five species of the genus Gluconobacter, namely G. albidus, G. cerinus, G. frateurii, G. oxydans (type species), and G. thailandicus. The type strain of G. asaii, NBRC 3276T (T=type strain) was included in the G. cerinus cluster, which is consistent with the report that G. asaii is a junior subjective synonym of G. cerinus. Existence of the G. albidus, G. cerinus, G. frateurii, G. oxydans, and G. thailandicus clusters was also recognized by the ITS sequence analysis. Both sequence analyses revealed that the G. cerinus and G. frateurii clusters were heterogeneous. The G. cerinus cluster comprised three strains of G. cerinus and one strain of G. frateurii, while the G. frateurii cluster included ten strains of G. frateurii, three of G. cerinus, and eleven of G. oxydans. These results suggest that phenotypic differences among Gluconobacter species are ambiguous and the species definition must be re-evaluated. The 16S rDNA and ITS sequences determined in this study are valuable for the identification and phylogenetic analysis of Gluconobacter species.
The entomogenous anamorphic fungus Isaria takamizusanensis has not been resolved clearly in its teleomorphic state. We succeeded in inducing ascostroma formation by incubating conidiomata of I. takamizusanensis on cicada adults in a moist chamber. We observed the ascostroma and conducted a phylogenetic analysis based on ITS rDNA and EF-1α genes. The morphology of the ascostroma was identical to that of Cordyceps ryogamimontana . In the phylogenetic tree inferred from EF-1α , the isolate from the partspores grouped with nine strains derived from conidia of I. takamizusanensis , which was distinct from a clade including Purpureocillium lilacinum . Moreover, a conidial structure identical to that of I. takamizusanensis was rediscovered on the holotype specimen of C. ryogamimontana . As a result, we propose a new name, Purpureocillium takamizusanense , which is a combination of the teleomorph–anamorph connection of C. ryogamimontana – I. takamizusanensis , in accordance with the ‘one fungus, one name’ concept of the International Code of Nomenclature for Algae, Fungi, and Plants (ICN).
The synthesis of TAN-1085 by Suzuki and co-workers is notable for its consecutive relay of central and axial chirality elements in three chirality transfer steps: central → axial (A + B → C), axial → axial (F → G) and axial → central (H → I).
Actinoplanes missouriensis Couch 1963 is a well-characterized member of the genus Actinoplanes, which is of morphological interest because its members typically produce sporangia containing motile spores. The sporangiospores are motile by means of flagella and exhibit chemotactic properties. It is of further interest that members of Actinoplanes are prolific sources of novel antibiotics, enzymes, and other bioactive compounds. Here, we describe the features of A. missouriensis 431T, together with the complete genome sequence and annotation. The 8,773,466 bp genome contains 8,125 protein-coding and 79 RNA genes.
We studied the DNA base composition and DNA-DNA hybridization of 41 strains of spore-forming, lactic acid-producing bacteria including Sporolactobacillus inulinus. The DNA base composition of 41 strains studied ranged from 42.4 to 50.2mol% guanine plus cytosine. The DNA of S. inulinus NRIC 1133T showed extremely low homology (1 to 5%) to the strains of Lactobacillus plantarum and Bacillus coagulans, but it showed somewhat higher homology to "Bacillus laevolacticus, " "Bacillus racemilacticus, " "Sporolactobacillus laevas, " "Sporolactobacillus laevas var, intermedius, " and "Sporolactobacillus racemicus" (20-50%). Catalase-negative strains of spore-forming lactic acid bacteria, including the type strain of S. inulinus, showed relatively high homology values to one another and further divided into five groups and considered to be members of the genus Sporolactobacillus.
The Hinai-dori is a breed of chicken native to Akita Prefecture, Japan.An F 2 resource population produced by crossing low-and high-growth lines of the Hinai-dori breed was analyzed to detect quantitative trait loci (QTL) for growth traits.Highly significant QTLs for body weight at 10 and 14 weeks of age and average daily gain between 4 and 10 weeks and between 10 and 14 weeks of age were accordingly mapped in a common region between ADL0198 and ABR0287 on chromosome 1 and between MCW0240 and ABR0622 on chromosome 4, respectively.A significant QTL for body weight at 4 weeks of age and a significant QTL for average daily gain between 0 and 4 weeks of age were mapped for the first time to the same region flanking ABR0204 and ABR0284 on chromosome 1.These QTLs are good candidates for application in the development of marker-assisted selection strategies for increasing growth efficiencies in the Hinai-dori breed and native breeds of chickens in Asia.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
