O.S. Kostiuk

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Tatyana Synelnyk

Taras Shevchenko National University of Kyiv

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О. Savchuk

Taras Shevchenko National University of Kyiv

СА

С. А. Суходоля

National Pirogov Memorial Medical University, Vinnytsya

ЛІ

Л. І. Остапченко

Taras Shevchenko National University of Kyiv

Olha Kravchenko

Taras Shevchenko National University of Kyiv

Nataliia Raksha

Taras Shevchenko National University of Kyiv

Tetyana Beregova

Taras Shevchenko National University of Kyiv

Tetiana Halenova

Taras Shevchenko National University of Kyiv

Cooperative Institutions

Taras Shevchenko National University of Kyiv

Bogomolets National Medical University

National Pirogov Memorial Medical University, Vinnytsya

St.John's Medical College Hospital

Danylo Zabolotny Institute of Microbiology and Virology

Ukrainian Medical Stomatological Academy

University of Southern California

Odessa National Medical University

International Iberian Nanotechnology Laboratory

Pirogov Russian National Research Medical University

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DISTRIBUTION OF SERINE PROTEASES IN BLOOD PLASMA AND PANCREAS IN CHRONIC PANCREATITIS AND ONCOPATHOLOGY

Fìzìologìčnij žurnal (2022)

Tatyana Synelnyk Olha Kravchenko O.S. Kostiuk О. Savchuk С. А. Суходоля

The aim of our study was to evaluate the trypsin-like serine proteases (TLPs) distribution between systemic circulation and pancreatic tissue and to investigate the peculiarities of their involvement in the extracellular matrix components degradation in patients with pancreatic pathologies with electrophoretic analysis methods using. Тhe Khmelnitsky Regional Clinical Hospital patients aged 28-89 were selected for this study: 20 people with chronic pancreatitis (group CP); 20 people with pancreatic cancer (group PC); 20 conditionally healthy persons (control). Blood plasma samples and pancreatic tissue homogenates were obtained from all the patients, from which the TLPs fractions were subsequently obtained by the affinity chromatography method. The study showed that TLPs content in the blood plasma of patients with pancreatic pathologies is higher, and in tissue homogenates is lower relative to the values of the corresponding indicators in the control. Disk-electrophoresis using showed that TLPs fractions obtained from the blood plasma of patients of all studied groups contain a lot of high molecular weight (HMW) proteins, while TLPs from the pancreatic tissue homogenates of patients with pancreatic pathologies mainly consists of low molecular weight (LMW) proteins. Enzyme-electrophoresis results showed that all TLPs fractions include enzymes with fibrinogenolytic, gelatinolytic and collagenolytic activity. In plasma, the first were represented by medium molecular weight (MMW) proteins, and the last two groups included a lot of HMW proteins as well as proteins with very high molecular weight. In homogenates, fibrinogenolytic activity was characteristic for LMW proteins only, whereas gelatinases and collagenases were represented by both MMW and LMW proteins. Our results indicate the differences in the TLPs fractions components obtained from blood plasma and pancreatic tissue of patients with investigated pathologies, as well as significant distinctions in the processes of extracellular matrix remodeling under СР and РС.

Blood plasma

10.15407/fz68.06.031

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Proteolytic activity in chronic pancreatitis and pancreatic cancer

Minerva Biotechnology and Biomolecular Research (2024)

Olha Kravchenko Tatyana Synelnyk O.S. Kostiuk Tetiana Halenova Nataliia Raksha

Proteolytic enzymes

10.23736/s2724-542x.24.03150-x

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THE TRIGGER COMPONENTS OF THE PROTEOLYTIC SYSTEM AND THEIR MODULATORS CONTENTS UNDER PANCREATIC PATHOLOGIES DEVELOPMENT

Fìzìologìčnij žurnal (2022)

Tatyana Synelnyk Olha Kravchenko O.S. Kostiuk О. Savchuk С. А. Суходоля

The content of the plasminogen activation system components (plasminogen, plasminogen tissue activator, and its inhibitor PAI-1) was investigated as well as the concentration of thrombomodulin, protein C, matrix metalproteinases (MMP -1, -2, -3, -8, -9, -10) and their inhibitor TIMP, growth factors content (such as transforming growth factor-β1, insulin-like growth factor-1, fibroblasts growth factor-2) and cytokine profile (interleukines IL-1β, -4, -6, -8, -10, tumor necrosis factor, interferon-γ in patients with chronic pancreatitis (CP) and pancreatic cancer (PC) were established. Khmelnitsky Regional Clinical Hospital patients aged 28-89 were selected for this study: 20 people with chronic pancreatitis (group CP); 20 people with pancreatic cancer (group PC); 20 conditionally healthy persons (control). Blood plasma samples and pancreatic tissue homogenates were obtained from all the patients. The studied indicators’ content in the experimental materials was determined by the enzyme-linked immunosorbent assay using appropriate antibodies. At the level of systemic circulation, a statistically significant increase was found in most of the studied parameters under the CP conditions. However, PC was characterized by an increase of two blood plasma indications only (thrombomodulin and protein C). In contrast, the pancreatic sample tissue examination for both aforementioned pathologies revealed significant changes in the content of most metalloproteinases and cytokines under PC. This indicates the development of metastasis, angiogenesis, and immunomodulation-aimed local biochemical processes in the affected organ.

Thrombomodulin

Plasminogen activator inhibitor-1

Zymogen

Proteolytic enzymes

10.15407/fz68.05.033

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