L Alföldi

Genetikum

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Studies on Bacterial Conjugational Crosses Via Model Populations

Acta Microbiologica et Immunologica Hungarica (2004)

L Alföldi

Model populations, simulating progenies obtained from Escherichia coli Hfr x F- crosses, are generated. Linkage relations and exchange frequencies are expressed in these populations in complex, in some cases, even paradoxical manner. It is demonstrated that the origin of the complexities is located in the particular allelic configuration of the genotypes, characteristic for all exconjugant and selected populations.

Linkage (software)

10.1556/amicr.51.2004.3.3

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Citations (2)

Serological types of Bacillus megaterium and their sensitivity to phages.

PubMed (1957)

G Ivánovics L Alföldi Á. Széll

Bacillus megaterium

Bacillus (shape)

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Citations (5)

Fusion of Microbial Protoplasts: Problems and Perspectives

Springer eBooks (1982)

L Alföldi

Protoplast

10.1007/978-1-4684-4142-0_7

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Citations (12)

[Lethal zygosis in crossing between colicinogenic and non-colicinogenic strains of Escherichia coli].

PubMed (1957)

L Alföldi F Jacob Wollman El

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Citations (7)

l -Serine Deaminase of Escherichia coli

Journal of Bacteriology (1968)

L Alföldi István Raskó Erzsébet Kerekes

The native l -serine deaminase ( l -serine hydrolyase, deaminating, EC 4.2.1.13) of Escherichia coli K-12, which seems to be a very labile protein, is rather stable in concentrated solution. Dilution rapidly inactivates it, but in the presence of a saturating concentration of l -serine the molecule is protected from inactivation. It is a very specific enzyme; l -serine is the sole substrate with a K m value of 6.60 × 10 −3 m. d -Serine and l -cysteine are competitive inhibitors. Substrate saturation curves of the native enzyme show sigmoid shape, whereas the enzyme liberated from the bacteria in the presence of l -serine exhibits normal Michaelis-Menten kinetics.

Enzyme Kinetics

10.1128/jb.96.5.1512-1518.1968

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Citations (43)

Observations on lysogenesis in B. megaterium and on megacine, the antibacterial principle of this bacillus species.

PubMed (1955)

G Ivánovics L Alföldi

Bacillus megaterium

Bacillus (shape)

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Citations (10)

L‐serine deaminating enzymes in Escherichia coli crude extracts

FEBS Letters (1970)

L Alföldi István Raskó

(a) The measured L-serine deaminating activity of a crude bacterial extract may originate from L-serine deaminase, from biosynthetic L-threonine deaminase, or from degradative L-serine deaminase. Nevertheless, the contribution of the individual enzymes can be determined.(b) About a half of the L-serine deaminating activity of wild type E. coli bacteria, grown in synthetic minimal medium, originates from L-serine deaminase and about half from biosynthetic L-threonine deaminase.(c) Ninety percent of L-serine deaminating activity of wild type E. coli bacteria, grown in yeast extract-tryptone medium, originates from L-serine deaminase, and the remainging ten percent from the degradative L-threonine deaminase.(d) Conditions have been established in which threonine deaminases are eliminated and the activity of L-serine deaminase alone could be measured, even in crude extracts.

10.1016/0014-5793(70)80004-7

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Citations (7)

Studies on Bacterial Conjugational Crosses Via Model Populations III.

Acta Microbiologica et Immunologica Hungarica (2005)

L Alföldi

Hfr x F- recombinational genome interactions are simulated by a computer program. Genotype spectrums of selected samples from Hfr x F- crosses of Escherichia coli, found in the published literature, are reproduced. Relevance of the findings for interpretation of conjugational results in Escherichia coli is discussed.

Hfr cell

Relevance

10.1556/amicr.52.2005.3-4.5

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Citations (0)

[Polyethylene glycol induction of Bacillus megaterium protoplast transformation by plasmid DNA].

PubMed (1980)

Vorob'eva Ip Khmel' Ia L Alföldi

Bacillus megaterium

Protoplast

Plasmid preparation

Source