Gabriel J. Mattos

Universidade Estadual de Londrina

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Elen Romão Sartori

Universidade Estadual de Londrina

Robert F. H. Dekker

Universidade Tecnológica Federal do Paraná

Aneli M. Barbosa‐Dekker

Universidade Estadual de Londrina

Carlos Alberto Rossi Salamanca‐Neto

Universidade Estadual de Londrina

Jessica Scremin

Universidade Estadual de Londrina

Eric Bakker

University of Geneva

Eduardo C. M. Barbosa

Karlsruhe Institute of Technology

Pedro H. C. Camargo

University of Helsinki

Bruna Coldibeli

Universidade Estadual de Londrina

Gustavo Galo Marcheafave

Universidade Estadual de Campinas (UNICAMP)

Cooperative Institutions

Universidade Estadual de Londrina

Entomological Society of Brazil

Universidade de São Paulo

University of Geneva

Manchester Metropolitan University

GTx (United States)

Universidade Tecnológica Federal do Paraná

Lakehead University

Chinese Academy of Sciences

Universidade Estadual de Campinas (UNICAMP)

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Integrated enzyme-linked immunosensor with biofunctionalized ion-selective membranes by pulstrode delivery of substrate

Biosensors and Bioelectronics X (2023)

Gabriel J. Mattos Eric Bakker

A new principle for electrochemical enzyme immunoassays is proposed where the enzyme substrate is selectively delivered by an electrochemical excitation pulse to the location where the biomolecular interaction takes place. This is achieved by covalent attachment of the capture antibody on the surface of a polymeric ion-selective membrane, which also serves to monitor the enzyme activity over time by open circuit chronopotentiometry. The membrane biofunctionalization was accomplished by using a click chemistry protocol in addition to N-hydroxysuccinimide ester crosslinking and was characterized using confocal microscopy and electrochemical impedance spectroscopy. To demonstrate the principle, a choline-oxidase labelled antibody served as the detection reporter in a sandwich immunoassay for the determination of human lysozyme in saliva in the range of 10–100 μg mL−1 with a detection limit of 0.7 μg mL−1 (3σ). The enzyme-immunocomplex at the sensing surface catalyzes the oxidation of choline, an ion marker to which the membrane is selective and that is released from the membrane by a pulstrode protocol (galvanostatic pulse of 1 s duration and 10 μA magnitude). The enzyme turnover is interrogated by measuring the open circuit potential of the electrode over time after the release pulse. A model based on diffusion and enzyme kinetics is developed to rationalize the sensing principle and to guide the experiments. The results with this integrated immunosensor compare well to that of a conventional enzyme-linked immunoassay.

Choline oxidase

10.1016/j.biosx.2023.100351

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Citations (1)

Serological diagnosis of strongyloidiasis in immunocompetent and immunosuppressed patients based on an electrochemical immunoassay using a flexible device allied to PLS-DA and ROC statistical tools

Sensors and Actuators B Chemical (2021)

Gabriel J. Mattos Gustavo Galo Marcheafave William H. Roldán Miguel J. Mattos Fabiana Martins de Paula

Strongyloidiasis

10.1016/j.snb.2021.131213

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Citations (3)

Laccase stabilized on β-D-glucan films on the surface of carbon black/gold nanoparticles: A new platform for electrochemical biosensing

Bioelectrochemistry (2019)

Gabriel J. Mattos Jaqueline Tobias Moraes Eduardo C. M. Barbosa Pedro H. C. Camargo Robert F. H. Dekker

Hydroquinone

Catechol

Repeatability

10.1016/j.bioelechem.2019.05.002

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Citations (30)

Boron-doped diamond film and multiple linear regression-based calibration applied to the simultaneous electrochemical determination of paracetamol, phenylephrine hydrochloride, and loratadine in fixed-dose combinations

Microchemical Journal (2020)

Carlos Alberto Rossi Salamanca‐Neto Gustavo Galo Marcheafave Gabriel J. Mattos Jaqueline Tobias Moraes Karolina Schwarzová‐Pecková

Loratadine

10.1016/j.microc.2020.105831

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Citations (11)

Ion transfer mediated by TEMPO in ionophore-doped thin films for multi-ion sensing by cyclic voltammetry

Analytica Chimica Acta (2024)

Gabriel J. Mattos Justine A. Rothen Nikolai Yu. Tiuftiakov Eric Bakker

We report here on the development of thin-layer ion-selective membranes containing lipophilic TEMPO as a phase-transfer redox mediator for the simultaneous detection of non-redoxactive ions. This redox probe was recently introduced by our group and provides ideal ion-transfer waves when the membrane is interrogated by cyclic voltammetry. To perform multianalyte detection in the same sensing film, plasticized PVC-based membranes were doped with lithium and potassium ionophores in addition to a lipophilic cation-exchanger. The ionophores allow for ion discrimination owing to the different ionophore-cation complexation constants and the oxidation of TEMPO to the oxoammonium form results in the selective transfer of lithium and potassium at different potentials. The resulting voltammograms have half-peak widths of 100 and 102 mV, and the peak separation between anodic and cathodic scans is 8 and 9 mV for lithium and potassium, respectively, close to theoretical expectations. High peak resolution was observed, and the ion-transfer waves are still distinguishable when the ion activities differ by three orders of magnitude. These parameters are remarkably better than those obtained with other redox probes, which is important for multianalyte detection in the same voltammetric scan. Optimized membranes showed independent Nernstian shifts (slopes of 59.23 mV and 54.8 mV for K+ and Li+, respectively) of the peak position for increasing ion concentrations. An idealized model for two ionophore-based membranes combining redox and phase-boundary potentials was applied to the proposed system with excellent correlation. Potassium and lithium ions were simultaneously detected in undiluted human serum samples with good accuracy and precision.

10.1016/j.aca.2024.342388

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Citations (2)

A Novel Strategy for Quantifying Clopidogrel Using Square‐wave Voltammetry and a Boron‐doped Diamond Film

Electroanalysis (2019)

Louise Oliveira Nascimento Jessica Scremin Gabriel J. Mattos Adriana de Oliveira Camargo Gomes Débora Nóbile Clausen

Abstract The voltammetric behavior of clopidogrel bisulfate (CLO), an antiplatelet agent, was investigated for the first time in the literature on a cathodically pretreated boron‐doped diamond electrode (CP‐BDDE) using cyclic (CV) and square‐wave voltammetry (SWV). It was observed an anodic peak for CLO, suitable for analytical purposes, at about 1.15 V (vs. Ag/AgCl (3.0 mol L −1 KCl)) by CV in Britton‐Robinson buffer solution (pH 5.0). On the physical‐chemical characterization of the interface phenomena, it was proved that electrode reaction of the analyte was controlled by a diffusion process. At optimized square‐wave parameters (pulse amplitude of 60 mV, frequency of 30 Hz and scan increment of 3 mV), the obtained analytical curve was linear for the CLO concentration range from 0.60 to 60.0 μmol L −1 , with a detection limit of 0.60 μmol L −1 . The simple, rapid and greener analytical method, based on CP‐BDDE electrochemical sensor, was successfully applied in real samples (pharmaceuticals and urine).

Square wave

10.1002/elan.201900219

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Citations (9)

Electrochemical biosensor based on botryosphaeran as a novel biomaterial for laccase immobilization

Resumos (2018)

Gabriel J. Mattos Aneli de Melo Barbosa Dekker Robert F. H. Dekker Pedro H. C. Camargo Elen R. Sartori

Biomaterial

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Lipophilic tetramethylpiperidine N-oxyl (TEMPO) as a phase-transfer redox mediator in thin films for anion and cation sensing

Electrochemistry Communications (2023)

Gabriel J. Mattos Nikolai Yu. Tiuftiakov Eric Bakker

This study successfully demonstrates the use of a lipophilic derivative of TEMPO, 1-oxyl-2,2,6,6-tetramethylpiperidin-4-yl stearate, as a redox mediator for ion transfer processes at the interface between two immiscible electrolyte solutions. The results prove that TEMPO exhibits outstanding performance for mediating anion and cation transfer across polymeric ion-selective membranes doped with lipophilic ion-exchangers. The redox reaction at the inner interface of the electrode was controlled by cyclic voltammetry and allowed to modulate the flux of different ions according to the Hofmeister selectivity series. The behavior of this system was shown to agree well with the theory combining redox and phase-boundary potentials by comparing experimental and calculated cyclic voltammograms. Model ions were used to show the ideal Nernstian shift of the peak position while changing the phase-boundary potential at the outer interface when ion activity is varied in the aqueous phase. We also showcase that limitations from electrochemical instabilities in anion transfer observed using other redox probes are now overcome.

Mediator

10.1016/j.elecom.2023.107603

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Citations (2)

Recent advances in electrochemical monitoring of epinephrine using carbon-based (bio)sensor devices for clinical applications

IOP Publishing eBooks (2022)

Gabriel J. Mattos Renan Silva Mariano Elen Romão Sartori

Electrochemical gas sensor

10.1088/978-0-7503-5127-0ch9

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Citations (0)

Electrochemical Characterization of the Laccase-Catalyzed Oxidation of 2,6-Dimethoxyphenol: an Insight into the Direct Electron Transfer by Enzyme and Enzyme-Mediator System

Applied Biochemistry and Biotechnology (2022)

Gabriel J. Mattos Jéseka G. Schirmann Carlos Alberto Rossi Salamanca‐Neto Robert F. H. Dekker Aneli M. Barbosa‐Dekker

10.1007/s12010-022-03838-3

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Citations (6)