Nuo Duan

Jiangnan University

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Zhouping Wang

Jiangnan University

163

Shijia Wu

Jiangnan University

156

Xianfeng Lin

State Key Laboratory of Food Science and Technology

Xiaoyuan Ma

Jiangnan University

Yu Xia

Jiangnan University

Yukun Huang

Xihua University

Xiujuan Chen

North China Electric Power University

You Zhou

Ningbo University

Shuo Qi

Nanjing Forestry University

Huajie Gu

Suzhou University of Science and Technology

Cooperative Institutions

Jiangnan University

108

State Key Laboratory of Food Science and Technology

Chinese Academy of Sciences

Zhejiang University

Shanghai Jiao Tong University

Wuxi Institute of Technology

Xi'an Jiaotong University

Ministry of Agriculture and Rural Affairs

Nanjing Medical University

Nanjing University

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A highly sensitive fluorescence resonance energy transfer aptasensor for staphylococcal enterotoxin B detection based on exonuclease-catalyzed target recycling strategy

Analytica Chimica Acta (2013)

Shijia Wu Nuo Duan Xiaoyuan Ma Yu Xia Hongxin Wang

Exonuclease III

Aptamer

10.1016/j.aca.2013.04.025

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Citations (55)

SERS aptasensor detection of Salmonella typhimurium using a magnetic gold nanoparticle and gold nanoparticle based sandwich structure

Analytical Methods (2016)

Xiaoyuan Ma Ying Liu Nixin Zhou Nuo Duan Shijia Wu

A SERS aptasensor for the detection ofSalmonella typhimuriumusing a magnetic gold nanoparticle and gold nanoparticle based sandwich structure.

10.1039/c6ay02623k

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Citations (29)

Colorimetric Aptasensor Based on Truncated Aptamer and Trivalent DNAzyme for Vibrio parahemolyticus Determination

Journal of Agricultural and Food Chemistry (2019)

Yuhan Sun Nuo Duan Pengfei Ma Yao Liang Xiaoyin Zhu

In this work, after optimizing the original aptamer sequence by truncation and site-directed mutagenesis, a simple and sensitive colorimetric aptasensor was established for detecting the widespread food-borne pathogen Vibrio parahemolyticus (V. parahemolyticus). The detection strategy was based on the competition for an V. parahemolyticus specific aptamer between its complementary DNA (cDNA) and V. parahemolyticus. The aptamer-conjugated magnetic nanoparticles (MNPs) were used as capture probes, and the G-quadruplex (G4) DNAzyme was employed as the signal amplifying element. Under optimal conditions, a wide linear detection range (from 102 to 107 cfu/mL) was available, and the detection limit could be as low as 10 cfu/mL. This method was also used to detect V. parahemolyticus in contaminated salmon samples, and the results showed good consistency with those obtained from standard plate counting method. Therefore, this novel aptasensor could be a good candidate for sensitive and selective detection of V. parahemolyticus without complicated operations.

Aptamer

10.1021/acs.jafc.8b06893

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Citations (90)

Aptamer-Based Lateral Flow Test Strip for Rapid Detection of Zearalenone in Corn Samples

Journal of Agricultural and Food Chemistry (2018)

Shijia Wu Lihong Liu Nuo Duan Qian Li You Zhou

An aptamer-based lateral flow test strip was developed for the detection of zearalenone (ZEN). This assay was based on the competition for the aptamer between ZEN and its complementary sequence. Several experimental conditions that could influence sensitivity have been investigated, including the concentration of aptamer and NaCl used in the probe preparation, the mole ratio of streptavidin and biotinylated DNA used in the preparation of test line and control line, and the loading quantity of gold nanoparticles–aptamer conjugates (AuNPs–Apt). Under the optimal experimental conditions, we successfully detected ZEN within a detection range of 5–200 ng/mL and the visual limit of detection of 20 ng/mL. This aptamer-based strip was successfully applied to the determination of ZEN in spiked corn samples, and the recoveries were from 93.4% to 114.2%. All detections can be achieved within 5 min. The results demonstrated that the developed aptamer-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of ZEN.

Aptamer

Streptavidin

10.1021/acs.jafc.7b05326

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Citations (169)

Hydrogel-integrated sensors for food safety and quality monitoring: Fabrication strategies and emerging applications

Critical Reviews in Food Science and Nutrition (2023)

Xianfeng Lin Han Yan Lehan Zhao Nuo Duan Zhouping Wang

Food safety is a global issue in public hygiene. The accurate, sensitive, and on-site detection of various food contaminants performs significant implications. However, traditional methods suffer from the time-consuming and professional operation, restricting their on-site application. Hydrogels with the merits of highly porous structure, high biocompatibility, good shape-adaptability, and stimuli-responsiveness offer a promising biomaterial to design sensors for ensuring food safety. This review describes the emerging applications of hydrogel-based sensors in food safety inspection in recent years. In particular, this study elaborates on their fabrication strategies and unique sensing mechanisms depending on whether the hydrogel is stimuli-responsive or not. Stimuli-responsive hydrogels can be integrated with various functional ligands for sensitive and convenient detection via signal amplification and transduction; while non-stimuli-responsive hydrogels are mainly used as solid-state encapsulating carriers for signal probe, nanomaterial, or cell and as conductive media. In addition, their existing challenges, future perspectives, and application prospects are discussed. These practices greatly enrich the application scenarios and improve the detection performance of hydrogel-based sensors in food safety detection.

Adaptability

10.1080/10408398.2023.2168619

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Citations (11)

Multifunctional Ce-MOF@PdNPs with colorimetric fluorescent electrochemical activity for ultrasensitive and accurate detection of diethylstilbestrol

Nano Research (2024)

Mingyue Ye Tingting Su Jinguo Li Xiaowan Chen Dichen Ying

10.1007/s12274-024-6951-4

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Citations (2)

Fluorometric determination of Vibrio parahaemolyticus using an F0F1-ATPase-based aptamer and labeled chromatophores

Microchimica Acta (2018)