7-(4-Hydroxyphenylacetamido)cephalosporanic acid (1) was transformed into 7-[1-oxaspiro(2.5)octa-6-oxo-4,7-diene-2-carboxamido]cephalosporanic acid (2) by laccase-catalyzed phenolic oxidation. 2 consisted of two diastereomers, named CXL-1 and CXL-2. CXL-2 was 10–30 times more active than CXL-1, although less active than 1.
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A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
The pcd gene from Flavobacterium lutescens IFO3084 encoding Delta'-piperideine-6-carboxylate dehydrogenase (PCD) was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence of PCD from F. lutescens IFO3084 showed strong similarity to that from Streptomyces clavuligerus. The molecular mass of the recombinant PCD was estimated to be approximately 58,000 Da by SDS-PAGE and native PAGE, which indicated that the enzyme molecule is a monomer. The in vitro analysis of L-alpha-aminoadipic acid (L-AAA) production showed that L-AAA is synthesized from L-lysine in two steps catalyzed by L-lysine 6-aminotransferase (LAT) and PCD from F. lutescens IFO3084.
Journal Article Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid Get access Hitosi Agematu, Hitosi Agematu Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, Japan Search for other works by this author on: Oxford Academic Google Scholar Norio Shibamoto, Norio Shibamoto Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, Japan Search for other works by this author on: Oxford Academic Google Scholar Hiroshi Nishida, Hiroshi Nishida Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, Japan Search for other works by this author on: Oxford Academic Google Scholar Rokuro Okamoto, Rokuro Okamoto Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, Japan Search for other works by this author on: Oxford Academic Google Scholar Takashi Shin, Takashi Shin Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, JapanDepartments of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda, Kumamoto 860, Japan Search for other works by this author on: Oxford Academic Google Scholar Sawao Murao Sawao Murao Central Research Laboratories, Merdan Co., 9–1, Johnan 4-chome, Fujisawa 251, JapanDepartments of Applied Microbial Technology, The Kumamoto Institute of Technology, Ikeda, Kumamoto 860, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 56, Issue 7, 1 January 1992, Pages 1176–1177, https://doi.org/10.1271/bbb.56.1176 Published: 01 January 1992 Article history Published: 01 January 1992 Received: 30 March 1992
Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
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