Ahmed M. Sayed

Nahda University

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Flow cytometric analysis of somatic cells and oxidant/antioxidant profile in dairy cows with subclinical mastitis

BULGARIAN JOURNAL OF VETERINARY MEDICINE (2018)

Nasr‐Eldin M. Aref Ahmed M. Sayed Ahmed Zahran Ghada Abdelaal E. Nasser

Flow cytometric analysis of somatic cells count (SCC) and oxidant/antioxidant status of cows with subclinical mastitis (SCM) were investigated in 75 lactating Holstein-Friesian cows (20 controls; 55 SCM cows).Milk from active quarters (n=280) and blood samples (n=75) were aseptically collected.Milk samples were subjected to California Mastitis Test (CMT), microbiologic examination and flow cytometric analysis.Blood and milk samples were analysed for malondialdehyde (MDA) concentrations and total antioxidant capacity (TAC).Microbiological examination revealed 3 major pathogens in a single or mixed infection: Streptococcus species (26.9%),Staphylococcus species (57.7%) and Escherichia coli (49.23%).Flow cytometric analysis showed significant increase (P<0.05) in inflammatory cells in milk of cows with SCM compared to control cows.Differential SCC was characterised by significant increase (P<0.05) in polymorphonuclear leukocyte (PMNL) counts compared to normal milk.Additionally, Staphylococcus infection alone or mixed with other lactopathogens resulted in dramatic increase in PMNL.There was a significant decrease (P<0.01) of TAC in plasma and milk (0.49 ± 0.04, 0.27 ± 0.03 mM/L, respectively) and significantly increased (P<0.01)serum and milk MDA concentrations (13.72 ± 0.3, 7.72 ± 0.17 nmol/mL, respectively) in SCM cows.In conclusion, differential SCC is a reliable index for early detection of SCM.Milk PMNL population can be used as a useful indicator for evaluation of udder infection.Additionally, a combination of MDA and TAC can provide complementary information about the health status of udder in dairy farms.

Somatic Cell Count

Subclinical infection

California mastitis test

Anti oxidant

10.15547/bjvm.1089

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Citations (3)

List of contributors

Elsevier eBooks (2024)

Yousef A. Abdelrady Majid Abdouss Osamah Aldaghri Muhammad Ali Nisar Ali

10.1016/b978-0-443-21891-0.00026-3

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Citations (0)

C. elegans as Model for the Study of High Glucose– Mediated Life Span Reduction

Diabetes (2009)

Andreas Schlotterer G Kukudov Farastuk Bozorgmehr Harald Hutter Xueliang Du

OBJECTIVE Establishing Caenorhabditis elegans as a model for glucose toxicity–mediated life span reduction. RESEARCH DESIGN AND METHODS C. elegans were maintained to achieve glucose concentrations resembling the hyperglycemic conditions in diabetic patients. The effects of high glucose on life span, glyoxalase-1 activity, advanced glycation end products (AGEs), and reactive oxygen species (ROS) formation and on mitochondrial function were studied. RESULTS High glucose conditions reduced mean life span from 18.5 ± 0.4 to 16.5 ± 0.6 days and maximum life span from 25.9 ± 0.4 to 23.2 ± 0.4 days, independent of glucose effects on cuticle or bacterial metabolization of glucose. The formation of methylglyoxal-modified mitochondrial proteins and ROS was significantly increased by high glucose conditions and reduced by mitochondrial uncoupling and complex IIIQo inhibition. Overexpression of the methylglyoxal–detoxifying enzyme glyoxalase-1 attenuated the life-shortening effect of glucose by reducing AGE accumulation (by 65%) and ROS formation (by 50%) and restored mean (16.5 ± 0.6 to 20.6 ± 0.4 days) and maximum life span (23.2 ± 0.4 to 27.7 ± 2.3 days). In contrast, inhibition of glyoxalase-1 by RNAi further reduced mean (16.5 ± 0.6 to 13.9 ± 0.7 days) and maximum life span (23.2 ± 0.4 to 20.3 ± 1.1 days). The life span reduction by glyoxalase-1 inhibition was independent from the insulin signaling pathway because high glucose conditions also affected daf-2 knockdown animals in a similar manner. CONCLUSIONS C. elegans is a suitable model organism to study glucose toxicity, in which high glucose conditions limit the life span by increasing ROS formation and AGE modification of mitochondrial proteins in a daf-2 independent manner. Most importantly, glucose toxicity can be prevented by improving glyoxalase-1–dependent methylglyoxal detoxification or preventing mitochondrial dysfunction.

Life span

Carbohydrate Metabolism

Model Organism

Lactoylglutathione lyase

10.2337/db09-0567

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Citations (269)

Impact of Copper II albumin complex on kidney impairment induced by aflatoxin B1 in rats

Egyptian Journal of Chemistry (2022)

Mona A. Mohamed Hend M. Abo‐Hiemad Ahmed Nassar Ahmed Shatat Mahmoud Soliman

10.21608/ejchem.2022.100401.4686

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Citations (1)

Dengue Virus Recruitment of Plasmin Proteolysis Increases Mosquito Midgut Internalization, Enhancing Infection Onset, and this Can Be Blocked by an Endogenous Kazal-Type Inhibitor

SSRN Electronic Journal (2019)

Karthik Ramesh Varsha Ashok Walvekar Benjamin Wong Ahmed M. Sayed Dorothée Missé

Deciphering the impact of dengue pathogenesis on vector transmission is critical for understanding virulence evolution, epidemiology and developing transmission-blocking tools. Dengue infection can alter blood coagulation and fibrinolysis pathways, resulting in severe hemorrhage and death. Here, we demonstrate that when plasmin, the human fibrinolytic factor, is added to blood meal, it enhances DENV infection in mosquito midgut, resulting in higher dissemination in mosquitoes. In addition, the mosquito midgut-expressed AaTI, a plasmin-selective mosquito Kazal-type inhibitor, reverted this enhanced infection by inhibiting plasmin proteolysis in the midgut lumen. Using bio-layer interferometry, we showed that DENV, plasmin and AaTI interact to form a tripartite complex. Eventually, plasmin supplementation in infectious blood meal increased midgut internalization of dextran molecules and this was reverted by AaTI. Our study demonstrates that: (a) DENV recruits plasmin to increase local proteolytic activity in the midgut, thus degrading the glyocalyx and enhancing DENV infection onset, and (b) AaTI can act as a transmission-blocking agent by inhibiting plasmin proteolysis. Our results indicate that dengue pathogenesis enhances DENV fitness by increasing its infectivity to mosquitoes.

Proteolysis

Internalization

10.2139/ssrn.3464034

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Ameliorate impacts of scopoletin against vancomycin-induced intoxication in rat model through modulation of Keap1-Nrf2/HO-1 and IκBα-P65 NF-κB/P38 MAPK signaling pathways: Molecular study, molecular docking evidence and network pharmacology analysis

International Immunopharmacology (2021)

Marwa M. Khalaf Samar M. Hassan Ahmed M. Sayed Amira M. Abo‐Youssef

Nephrotoxicity

scopoletin

KEAP1

10.1016/j.intimp.2021.108382

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Citations (22)

Long-term correction of hemophilia B through CRISPR/Cas9 induced homology-independent targeted integration

Journal of genetics and genomics/Journal of Genetics and Genomics (2022)

Xi Chen Xuran Niu Yang Liu Rui Zheng Lei Yang

Factor IX

Homology

Clotting factor

10.1016/j.jgg.2022.06.001

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Citations (14)

C. elegans as model for the study of high glucose mediated lifespan reduction Running title: Glucose regulates lifespan in C. elegans

Andreas Schlotterer G Kukudov Farastuk Bozorgmehr Harald Hutter Xueliang Du

Source

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Citations (1)

Synthesis, biological evaluation and molecular docking study of some new 4-aminosalicylic acid derivatives as anti-inflammatory and antimycobacterial agents

Bioorganic Chemistry (2023)

Maha Q. M. Qahtan Etify A. Bakhite Jyothi Kumari Ahmed M. Sayed Mahmoud Kandeel

Antimycobacterial

Docking (animal)

Aminosalicylic acid

Anti-inflammatory

10.1016/j.bioorg.2023.106344

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Citations (5)

Flavonoids-mediated TLR4 Inhibition as a Promising Therapy for Renal Diseases

Combinatorial Chemistry & High Throughput Screening (2023)

Ayman M. Mahmoud Ahmed M. Sayed Ahmed F. Ahmeda Esraa K. Abd‐alhameed Shimaa H. Salem

Abstract: Toll-like receptors (TLRs) control both innate and adaptive immunity with a wide expression on renal epithelial cells and leukocytes. Activation of TLRs results in the production of cytokines, chemokines and interferons along with activation of the transcription factor NF-κB, resulting in inflammatory perturbations. TLR4 signaling pathway is the most extensively studied of TLRs. TLR4 is expressed on renal microvascular endothelial and tubular epithelial cells. So, targeting TLR4 modulation could be a therapeutic approach to attenuate kidney diseases that are underlined by inflammatory cascade. Medicinal plants with anti-inflammatory activities display valuable effects and are employed as alternative sources to alleviate renal disease linked with inflammation. Flavonoids and other phytochemicals derived from traditional medicines possess promising pharmacological activities owing to their relatively cheap and high safety profile. Our review focuses on the potent anti-inflammatory activities of twenty phytochemicals to verify if their potential promising renoprotective effects are related to suppression of TLR4 signaling in different renal diseases, including sepsis-induced acute kidney injury, renal fibrosis, chemotherapy-induced nephrotoxicity, diabetic nephropathy and renal ischemia/reperfusion injury. Additionally, molecular docking simulations were employed to explore the potential binding affinity of these phytochemicals to TLR4 as a strategy to attenuate renal diseases associated with activated TLR4 signaling.

HMGB1

Nephrotoxicity

10.2174/1386207326666230116124532

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Citations (1)